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Rabbit Recombinant Monoclonal 53BP1 antibody. Suitable for IP, WB, IHC-P, Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TP53BP1 aa 350-400.

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Images

Immunoprecipitation - Anti-53BP1 antibody [BL-250-1H11] (AB243868), expandable thumbnail
  • Western blot - Anti-53BP1 antibody [BL-250-1H11] (AB243868), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] (AB243868), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] (AB243868), expandable thumbnail
  • Flow Cytometry - Anti-53BP1 antibody [BL-250-1H11] (AB243868), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human TP53BP1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt
Human
Tested
Tested
Expected
Tested
Mouse
Expected
Expected
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 20μl/mg lysate.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/500.00000

Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for Formalin-fixed, paraffin-embedded tissue sections

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µL for 106 Cells

Notes

-

Species

Mouse

Dilution info

1 µL for 106 Cells

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).

Alternative names

TP53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1, TP53BP1

Recommended products

Rabbit Recombinant Monoclonal 53BP1 antibody. Suitable for IP, WB, IHC-P, Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TP53BP1 aa 350-400.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human TP53BP1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888
Clone number

BL-250-1H11

Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

53BP1 also known as p53-binding protein 1 is a protein that plays an important role in DNA damage response. The 53BP1 protein has a molecular weight of approximately 213 kDa. It is a nuclear protein and it mainly expresses in cells during the DNA damage repair processes. Researchers commonly utilize immunofluorescence and anti-53BP1 antibodies to detect its presence especially for studying DNA repair mechanisms. 53BP1 proves important for maintenance of genomic stability.

Biological function summary

As a DNA damage response mediator 53BP1 functions to recognize and bind to sites of double-strand breaks in DNA promoting non-homologous end joining (NHEJ). This protein is a component of a larger protein complex that recruits factors necessary for DNA repair. Its interaction with DNA lesions also involves chromatin remodeling aiming to facilitate successful repair of the DNA. 53BP1 works in concert with other proteins such as H2AX and MDC1.

Pathways

Researchers observe 53BP1 in processes such as the DNA damage checkpoint and repair pathways. This protein significantly contributes to the pathways involved in maintaining cell cycle checkpoints. It interacts with proteins like ATM and BRCA1 coordinating the cellular response to DNA damage and ensuring that genomic integrity checks occur properly before cell cycle progression continues. These interactions place 53BP1 at a pivotal position in decision-making between repair pathways.

Associated diseases and disorders

Researchers often focus on 53BP1's involvement in cancer development and therapy resistance due to its role in DNA repair. Alterations or deficiencies in 53BP1 levels can contribute to increased susceptibility to tumor development affecting processes like breast cancer and ovarian cancer through its relationship with BRCA1. The improper function of 53BP1 in DNA repair pathways may lead to an accumulation of genetic mutations driving tumorigenesis and impacting the efficacy of certain cancer treatments.

Product promise

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7 product images

  • Immunoprecipitation - Anti-53BP1 antibody [BL-250-1H11] (ab243868), expandable thumbnail

    Immunoprecipitation - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    53BP1 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243868 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243868 at 1/1000 dilution. Lane 1: ab243868 IP in HEK-293T cell lysate.

    Lane 2: Contol IgG in HEK-293T cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

    All lanes: Immunoprecipitation - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    Predicted band size: 214 kDa

  • Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868), expandable thumbnail

    Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    Western blot analysis using ab243868 at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate (15 µg).

    Lane 2: HEK-293T whole cell lysate (15 µg).

    Lane3: MCF7 whole cell lysate (15 µg).

    Lane 4: HepG2 whole cell lysate (15 µg).

    Lane 5: A549 whole cell lysate (15 µg).

    Lane 6: SW620 whole cell lysate (15 µg).

    Lane 7: SK whole cell lysate (15 µg).

    Lane 8: Jurkat whole cell lysate (15 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds

    All lanes: Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    Predicted band size: 214 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] (ab243868), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    Formalin-fixed, paraffin-embedded mouse renal cell carcinoma stained using ab243868 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] (ab243868), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained using ab243868 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

  • Flow Cytometry - Anti-53BP1 antibody [BL-250-1H11] (ab243868), expandable thumbnail

    Flow Cytometry - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    Flow cytometry overlay histogram of 4% formaldehyde fixed mIMCD-3 cells permeabilized with 90% methanol labeling human 53BP1 with ab243868 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details, Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).

  • Flow Cytometry - Anti-53BP1 antibody [BL-250-1H11] (ab243868), expandable thumbnail

    Flow Cytometry - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    Flow cytometry overlay histogram of 4% formaldehyde fixed HDLM-2 cells permeabilized with 90% methanol labeling human 53BP1 with ab243868 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype
    control (unshaded). Secondary antibody details, Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886)

  • Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868), expandable thumbnail

    Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868)

    All lanes: Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 15 µg

    Lane 2: HEK293T whole cell lysate at 15 µg

    Lane 3: MCF-7 whole cell lysate at 15 µg

    Lane 4: Hep-G2 whole cell lysate at 15 µg

    Lane 5: A-549 whole cell lysate at 15 µg

    Lane 6: SW620 whole cell lysate at 15 µg

    Lane 7: SK-MEL-28 whole cell lysate at 15 µg

    Lane 8: Jurkat whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Developed using the ECL technique.

    Predicted band size: 213 kDa

    Observed band size: 350 kDa

    Exposure time: 10s

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