JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB70323

Anti-53BP1 (phospho S25) antibody

2

(3 Reviews)

|

(7 Publications)

Rabbit Polyclonal 53BP1 phospho S25 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TP53BP1 phospho S25.

View Alternative Names

TP53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1, TP53BP1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 (phospho S25) antibody (AB70323)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 (phospho S25) antibody (AB70323)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse teratoma (right) tissues labelling 53BP1 (phospho S24) with ab70323 at 1/1000 (1µg/ml). Detection : DAB.

Western blot - Anti-53BP1 (phospho S25) antibody (AB70323)
  • WB

Unknown

Western blot - Anti-53BP1 (phospho S25) antibody (AB70323)

All lanes:

Western blot - Anti-53BP1 (phospho S25) antibody (ab70323) at 0.1 µg/mL

Lane 1:

Whole cell lysate from 293T cells, untreated at 50 µg

Lane 2:

Whole cell lysate from 293T cells treatedwith NCS (200 ng/ml for 1h) at 50 µg

Predicted band size: 214 kDa

Observed band size: >220 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TP53BP1 phospho S25. The exact immunogen used to generate this antibody is proprietary information.

Q12888

style
left-column

Alternative Products

Primary Antibodies

AB36823

Anti-53BP1 antibody

Immunoprecipitation - Anti-53BP1 antibody (AB36823)

  • 4
  • 12
Primary Antibodies

AB272067

Anti-53BP1 antibody [BL-250-1H11] - BSA free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] - BSA free (AB272067)

  • 0
  • 0
Primary Antibodies

AB243868

Anti-53BP1 antibody [BL-250-1H11]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] (AB243868)

  • 0
  • 0
Primary Antibodies

AB175933

Anti-53BP1 antibody [EPR2172(2)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody [EPR2172(2)] (AB175933)

  • 5
  • 3
Primary Antibodies

AB175188

Anti-53BP1 antibody [EPR2173(2)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [EPR2173(2)] (AB175188)

  • 5
  • 1
Primary Antibodies

AB237174

Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (AB237174)

  • 0
  • 0
Primary Antibodies

AB222232

Anti-53BP1 antibody [EPR2172(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody [EPR2172(2)] - BSA and Azide free (AB222232)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 18
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Baboon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

53BP1 also known as p53-binding protein 1 is a protein that plays an important role in DNA damage response. The 53BP1 protein has a molecular weight of approximately 213 kDa. It is a nuclear protein and it mainly expresses in cells during the DNA damage repair processes. Researchers commonly utilize immunofluorescence and anti-53BP1 antibodies to detect its presence especially for studying DNA repair mechanisms. 53BP1 proves important for maintenance of genomic stability.
Biological function summary

As a DNA damage response mediator 53BP1 functions to recognize and bind to sites of double-strand breaks in DNA promoting non-homologous end joining (NHEJ). This protein is a component of a larger protein complex that recruits factors necessary for DNA repair. Its interaction with DNA lesions also involves chromatin remodeling aiming to facilitate successful repair of the DNA. 53BP1 works in concert with other proteins such as H2AX and MDC1.

Pathways

Researchers observe 53BP1 in processes such as the DNA damage checkpoint and repair pathways. This protein significantly contributes to the pathways involved in maintaining cell cycle checkpoints. It interacts with proteins like ATM and BRCA1 coordinating the cellular response to DNA damage and ensuring that genomic integrity checks occur properly before cell cycle progression continues. These interactions place 53BP1 at a pivotal position in decision-making between repair pathways.

Researchers often focus on 53BP1's involvement in cancer development and therapy resistance due to its role in DNA repair. Alterations or deficiencies in 53BP1 levels can contribute to increased susceptibility to tumor development affecting processes like breast cancer and ovarian cancer through its relationship with BRCA1. The improper function of 53BP1 in DNA repair pathways may lead to an accumulation of genetic mutations driving tumorigenesis and impacting the efficacy of certain cancer treatments.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed : 12364621, PubMed : 17190600, PubMed : 21144835, PubMed : 22553214, PubMed : 23333306, PubMed : 27153538, PubMed : 28241136, PubMed : 31135337, PubMed : 37696958). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed : 22553214, PubMed : 23333306, PubMed : 23727112, PubMed : 27153538, PubMed : 31135337). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed : 28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed : 17190600, PubMed : 23760478, PubMed : 27153538, PubMed : 28241136). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed : 23345425). Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed : 23727112).
See full target information TP53BP1 phospho S25
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114694 PubMed39196777

2024

RNase L-induced bodies sequester subgenomic flavivirus RNAs to promote viral RNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

J Monty Watkins,James M Burke

Proceedings of the National Academy of Sciences of the United States of America 119:e2208506119 PubMed36256824

2022

FLASH X-ray spares intestinal crypts from pyroptosis initiated by cGAS-STING activation upon radioimmunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Shi,Yiwei Yang,Wei Zhang,Jianxin Wang,Dexin Xiao,Huangge Ren,Tingting Wang,Feng Gao,Zhen Liu,Kui Zhou,Peng Li,Zheng Zhou,Peng Zhang,Xuming Shen,Yu Liu,Jianheng Zhao,Zhongmin Wang,Fenju Liu,Chunlin Shao,Dai Wu,Haowen Zhang

Cancer letters 534:215612 PubMed35259458

2022

ETS-related gene (ERG) undermines genome stability in mouse prostate progenitors via Gsk3β dependent Nkx3.1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Lorenzoni,Dario De Felice,Giulia Beccaceci,Giorgia Di Donato,Veronica Foletto,Sacha Genovesi,Arianna Bertossi,Francesco Cambuli,Francesca Lorenzin,Aurora Savino,Lidia Avalle,Alessia Cimadamore,Rodolfo Montironi,Veronica Weber,Francesco Giuseppe Carbone,Mattia Barbareschi,Francesca Demichelis,Alessandro Romanel,Valeria Poli,Giannino Del Sal,Marianna Kruithof-de Julio,Marco Gaspari,Alessandro Alaimo,Andrea Lunardi

Science (New York, N.Y.) 370: PubMed33122357

2020

Multi-omics analyses of radiation survivors identify radioprotective microbes and metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Guo,Wei-Chun Chou,Yunjia Lai,Kaixin Liang,Jason W Tam,W June Brickey,Liang Chen,Nathan D Montgomery,Xin Li,Lauren M Bohannon,Anthony D Sung,Nelson J Chao,Jonathan U Peled,Antonio L C Gomes,Marcel R M van den Brink,Matthew J French,Andrew N Macintyre,Gregory D Sempowski,Xianming Tan,R Balfour Sartor,Kun Lu,Jenny P Y Ting

Molecular cell 77:26-38.e7 PubMed31653568

2019

53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Elsa Callen,Dali Zong,Wei Wu,Nancy Wong,Andre Stanlie,Momoko Ishikawa,Raphael Pavani,Lavinia C Dumitrache,Andrea K Byrum,Carlos Mendez-Dorantes,Paula Martinez,Andres Canela,Yaakov Maman,Amanda Day,Michael J Kruhlak,Maria A Blasco,Jeremy M Stark,Nima Mosammaparast,Peter J McKinnon,André Nussenzweig

Experimental dermatology 29:29-38 PubMed31519066

2019

Voriconazole enhances UV-induced DNA damage by inhibiting catalase and promoting oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Vivian Lee,Michael D Gober,Hasan Bashir,Conor O'Day,Ian A Blair,Clementina Mesaros,Liwei Weng,Andrew Huang,Aaron Chen,Rachel Tang,Vince Anagnos,JiLon Li,Sophie Roling,Emilija Sagaityte,Andrew Wang,Chenyan Lin,Christopher Yeh,Cem Atillasoy,Christine Marshall,Tzvete Dentchev,Todd Ridky,John T Seykora

Oncology reports 40:3663-3673 PubMed30272350

2018

Mangiferin induces radiosensitization in glioblastoma cells by inhibiting nonhomologous end joining.

Applications

Unspecified application

Species

Unspecified reactive species

Faguang Mu,Ting Liu,Hanrui Zheng,Xiaofang Xie,Tiantian Lei,Xia He,Suya Du,Rongsheng Tong,Yi Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com