Knockout Tested Rabbit Recombinant Monoclonal 53BP1 phospho T543 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Human samples.
Images
![Dot Blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--dot-blot-img427448.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317)")
![Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--western-blot-img427420.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317)")
![Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--western-blot-img427419.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317)")
![Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--western-blot-img427418.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (AB316317)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | ICC/IF | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:17190600, PubMed:21144835, PubMed:22553214, PubMed:23333306, PubMed:27153538, PubMed:28241136, PubMed:31135337, PubMed:37696958). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23333306, PubMed:23727112, PubMed:27153538, PubMed:31135337). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:17190600, PubMed:23760478, PubMed:27153538, PubMed:28241136). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Alternative names
TP53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1, TP53BP1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal 53BP1 phospho T543 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27059-168
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316317 is the carrirer-free version of Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
53BP1 also known as p53-binding protein 1 is a protein that plays an important role in DNA damage response. The 53BP1 protein has a molecular weight of approximately 213 kDa. It is a nuclear protein and it mainly expresses in cells during the DNA damage repair processes. Researchers commonly utilize immunofluorescence and anti-53BP1 antibodies to detect its presence especially for studying DNA repair mechanisms. 53BP1 proves important for maintenance of genomic stability.
- Biological function summary
As a DNA damage response mediator 53BP1 functions to recognize and bind to sites of double-strand breaks in DNA promoting non-homologous end joining (NHEJ). This protein is a component of a larger protein complex that recruits factors necessary for DNA repair. Its interaction with DNA lesions also involves chromatin remodeling aiming to facilitate successful repair of the DNA. 53BP1 works in concert with other proteins such as H2AX and MDC1.
- Pathways
Researchers observe 53BP1 in processes such as the DNA damage checkpoint and repair pathways. This protein significantly contributes to the pathways involved in maintaining cell cycle checkpoints. It interacts with proteins like ATM and BRCA1 coordinating the cellular response to DNA damage and ensuring that genomic integrity checks occur properly before cell cycle progression continues. These interactions place 53BP1 at a pivotal position in decision-making between repair pathways.
- Associated diseases and disorders
Researchers often focus on 53BP1's involvement in cancer development and therapy resistance due to its role in DNA repair. Alterations or deficiencies in 53BP1 levels can contribute to increased susceptibility to tumor development affecting processes like breast cancer and ovarian cancer through its relationship with BRCA1. The improper function of 53BP1 in DNA repair pathways may lead to an accumulation of genetic mutations driving tumorigenesis and impacting the efficacy of certain cancer treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Dot Blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--dot-blot-img427448.jpg "Dot Blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)")
Dot Blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)
This data was developed using Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316, the same antibody clone in a different buffer formulation.
Dot blot analysis of 53BP1 (phospho T543) using Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316 at 1:1000 (0.504 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
All lanes: Dot Blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] (Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316) at 1/1000 dilution
Lane 1: 53BP1 (phospho T543) peptide a
Lane 2: 53BP1 (phospho T543) peptide b
Lane 3: 53BP1 non-phospho peptide
Lane 4: 53BP1 (phospho T548) peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 3min
![Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--western-blot-img427420.jpg "Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)")
Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)
This data was developed using Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316, the same antibody clone in a different buffer formulation.
The identity of the band at 130 kDa is unknown.
The bands beneath the target band (350 kDa) are likely to be degraded target fragments .
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
In Western blot, Anti-53BP1 antibody [EPR2172 (2)] (Anti-53BP1 antibody [EPR2172(2)] ab175933) staining at 1/1000 dilution.
All lanes: Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] (Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316) at 1/1000 dilution
Lane 1: Untreated HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: HeLa treated with /ml Calyculin A for 15 minutes, whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 350 kDa, 124 kDa
Exposure time: 180s
![Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--western-blot-img427419.jpg "Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)")
Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)
This data was developed using Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316, the same antibody clone in a different buffer formulation.
The identity of the band at 130 kDa is unknown.
The bands beneath the target band (350 kDa) are likely to be degraded target fragments which are with total protein control Anti-53BP1 antibody [EPR2172(2)] ab175933 .
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
In Western blot, Anti-53BP1 antibody [EPR2172 (2)] (Anti-53BP1 antibody [EPR2172(2)] ab175933) staining at 1/1000 dilution.
All lanes: Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] (Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316) at 1/1000 dilution
Lane 1: Untreated U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 2: U-2 OS treated with 10um Camptothecin for 1 hour, whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 3: Untreated U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 4: U-2 OS treated with 10um Camptothecin for 1 hour, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 350 kDa, 124 kDa
Exposure time: 48s
![Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317), expandable thumbnail](https://content.abcam.com/products/images/53bp1-phospho-t543-antibody-epr27059-168-bsa-and-azide-free-ab316317--western-blot-img427418.jpg "Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)")
Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] - BSA and Azide free (ab316317)
This data was developed using Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316, the same antibody clone in a different buffer formulation.
The identity of the band at 130 kDa is unknown.
The bands beneath the target band (350 kDa) are likely to be degraded target fragments which are with total protein control Anti-53BP1 antibody [EPR2172(2)] ab175933.
In Western blot, Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316 was shown to bind specifically to 53BP1 (phospho T543). A band was observed at 350 kDa in Calyculin A treated wild-type HAP1 cell lysates whereas loss of signal was observed in the 53BP1 knockout cell line.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
In Western blot, Anti-53BP1 antibody [EPR2172 (2)] (Anti-53BP1 antibody [EPR2172(2)] ab175933) staining at 1/1000 dilution.
All lanes: Western blot - Anti-53BP1 (phospho T543) antibody [EPR27059-168] (Anti-53BP1 (phospho T543) antibody [EPR27059-168] ab316316) at 1/1000 dilution
Lane 1: Untreated wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 2: Wild-type HAP1 treated with 100nM Calycin A for 30 minutes, whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 3: Untreated TP53BP1 knockout HAP1 whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 4: TP53BP1 knockout HAP1 treated with 100nM Calycin A for 30 minutes, whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 5: Untreated wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 6: Wild-type HAP1 treated with 100nM Calycin A for 30 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 7: Untreated TP53BP1 knockout HAP1 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 8: TP53BP1 knockout HAP1 treated with 100nM Calycin A for 30 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 350 kDa, 124 kDa
Exposure time: 48s
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