AB236448

Anti-53BP2/ASPP2/BBP antibody

Name: Anti-53BP2/ASPP2/BBP antibody (ab236448) | Abcam
Brand: Abcam Limited
SKU: AB236448
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal 53BP2/ASPP2/BBP antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TP53BP2.

View Alternative Names

ASPP2, BBP, TP53BP2, Apoptosis-stimulating of p53 protein 2, Bcl2-binding protein, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, Bbp, 53BP2, p53-binding protein 2, p53BP2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (AB236448)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (AB236448)

2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Right panel shows the merged images.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (AB236448)

Supplier Data

Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (AB236448)

53BP2/ASPP2/BBP was immunoprecipitated from ~400 μg U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate with with 3 μl ab236448. Western blot was performed from the immunoprecipitate using ab236448 at 1/2000 dilution.

Lane 1 : U-2 OS cell lysate.

Lane 2 : Control IP in U-2 OS cell lysate.

Lane 3 : ab236448 IP in U-2 OS cell lysate.

All lanes:

Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (ab236448)

Predicted band size: 126 kDa

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Supplier Data

Western blot - Anti-53BP2/ASPP2/BBP antibody (AB236448)

All lanes:

Western blot - Anti-53BP2/ASPP2/BBP antibody (ab236448) at 1/2000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA

Lane 2:

U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with 53BP2/ASPP2/BBP siRNA

Lane 3:

Mouse MEF cell lysate

Lane 4:

Rat INS-1 cell lysate

Predicted band size: 126 kDa

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Lab

Western blot - Anti-53BP2/ASPP2/BBP antibody (AB236448)

Western blot : Rabbit Polyclonal to 53BP2/ASPP2/BBP ab236448 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 126-150 kDa in Wild-type A549 cell lysates with no signal observed at this size in TP53BP2 knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-53BP2/ASPP2/BBP antibody (ab236448) at 1/5000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

TP53BP2 knockout A549 at 20 µg

Lane 3:

THP-1 at 20 µg

Lane 4:

Raji at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 126 kDa

Observed band size: 126-150 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human TP53BP2. The exact immunogen used to generate this antibody is proprietary information.

Q13625

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 3 μl to ~400 μg of extact", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

53BP2 also known as ASPP2 or BBP is a protein that interacts with the p53 tumor suppressor. It has a mass of approximately 140 kDa and is expressed in various tissues including the brain liver and lung. Mechanically 53BP2 modulates the activity of p53 influencing cellular apoptosis. The protein consists of several domains such as ankyrin repeats and SH3 domain that facilitate its interaction with multiple partners enhancing its regulatory function.

Biological function summary

53BP2 plays a role in apoptosis and cell cycle control. It acts as a co-activator in the p53 signaling pathway promoting the pro-apoptotic functions of p53. This protein forms part of a complex network involving p53 and other regulatory molecules which ultimately governs important cellular processes like growth arrest and death. By enhancing p53 transcriptional activity 53BP2 aids in maintaining cellular homeostasis and preventing uncontrolled cell proliferation.

Pathways

53BP2 is primarily involved in the p53 signaling and apoptosis pathways. It works closely with key proteins such as p53 and Bcl-2 enhancing the apoptotic response. In the p53 pathway 53BP2 strengthens p53's ability to transactivate genes essential for apoptosis while in the apoptotic pathway it interacts with members of the Bcl-2 family to regulate mitochondrial events leading to cell death.

53BP2's function is relevant in cancer and neurodegenerative diseases. In cancer dysregulation of 53BP2 function or expression can lead to impaired apoptotic response contributing to tumorigenesis. It is linked to proteins like p53 and Bcl-2 in the context of cancer. In neurodegenerative diseases alterations in 53BP2 activity can affect neuronal cell survival with potential connections to proteins involved in synaptic pathways and apoptotic regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53 (PubMed : 12524540). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of NAE1 to conjugate NEDD8 to CUL1, and thereby decreases NAE1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.

See full target information TP53BP2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 12:e70096 PubMed39424429

2024

Statin suppresses the development of excessive intimal proliferation in a Kawasaki disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Motoji,Ryuji Fukazawa,Ryosuke Matsui,Makoto Watanabe,Yoshiaki Hashimoto,Noriko Nagi-Miura,Tadashi Kitamura,Kagami Miyaji

Genomics, proteomics & bioinformatics 20:597-613 PubMed33607295

2021

Phosphoproteomics Reveals the AMPK Substrate Network in Response to DNA Damage and Histone Acetylation.

Applications

Species

Mouse

Yuejing Jiang,Xiaoji Cong,Shangwen Jiang,Ying Dong,Lei Zhao,Yi Zang,Minjia Tan,Jia Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-53BP2/ASPP2/BBP antibody

Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (AB236448)

Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (AB236448)

Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (AB236448)

Western blot - Anti-53BP2/ASPP2/BBP antibody (AB236448)

Western blot - Anti-53BP2/ASPP2/BBP antibody (AB236448)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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