AB70548

Anti-53BP2/ASPP2/BBP antibody

Name: Anti-53BP2/ASPP2/BBP antibody (ab70548) | Abcam
Brand: Abcam Limited
SKU: AB70548
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal 53BP2/ASPP2/BBP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TP53BP2.

View Alternative Names

ASPP2, BBP, TP53BP2, Apoptosis-stimulating of p53 protein 2, Bcl2-binding protein, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, Bbp, 53BP2, p53-binding protein 2, p53BP2

2 Images

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Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (AB70548)

1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70548 at 3ug/mg of lysate(lane 1) or a control IgG (lane 2). Fr the subsequent blot, 20% of immnunoprecipitate was loaded per lane, and probed with ab70548 at 1ug/ml. Detection : chemiluminescence with exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (ab70548)

Predicted band size: 126 kDa

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Western blot - Anti-53BP2/ASPP2/BBP antibody (AB70548)

All lanes:

Western blot - Anti-53BP2/ASPP2/BBP antibody (ab70548) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 126 kDa

Observed band size: 170 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TP53BP2. The exact immunogen used to generate this antibody is proprietary information.

Q13625

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

53BP2 also known as ASPP2 or BBP is a protein that interacts with the p53 tumor suppressor. It has a mass of approximately 140 kDa and is expressed in various tissues including the brain liver and lung. Mechanically 53BP2 modulates the activity of p53 influencing cellular apoptosis. The protein consists of several domains such as ankyrin repeats and SH3 domain that facilitate its interaction with multiple partners enhancing its regulatory function.

Biological function summary

53BP2 plays a role in apoptosis and cell cycle control. It acts as a co-activator in the p53 signaling pathway promoting the pro-apoptotic functions of p53. This protein forms part of a complex network involving p53 and other regulatory molecules which ultimately governs important cellular processes like growth arrest and death. By enhancing p53 transcriptional activity 53BP2 aids in maintaining cellular homeostasis and preventing uncontrolled cell proliferation.

Pathways

53BP2 is primarily involved in the p53 signaling and apoptosis pathways. It works closely with key proteins such as p53 and Bcl-2 enhancing the apoptotic response. In the p53 pathway 53BP2 strengthens p53's ability to transactivate genes essential for apoptosis while in the apoptotic pathway it interacts with members of the Bcl-2 family to regulate mitochondrial events leading to cell death.

53BP2's function is relevant in cancer and neurodegenerative diseases. In cancer dysregulation of 53BP2 function or expression can lead to impaired apoptotic response contributing to tumorigenesis. It is linked to proteins like p53 and Bcl-2 in the context of cancer. In neurodegenerative diseases alterations in 53BP2 activity can affect neuronal cell survival with potential connections to proteins involved in synaptic pathways and apoptotic regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53 (PubMed : 12524540). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of NAE1 to conjugate NEDD8 to CUL1, and thereby decreases NAE1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.

See full target information TP53BP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1473 PubMed34737813

2021

Tea polyphenols alleviate hydrogen peroxide-induced oxidative stress damage through the Mst/Nrf2 axis and the Keap1/Nrf2/HO-1 pathway in murine RAW264.7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Li,Zhaoyan Qiu,Yan Wang,Chunyan Guo,Xu Cai,Yandong Zhang,Li Liu,Hongkun Xue,Jintian Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-53BP2/ASPP2/BBP antibody

Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (AB70548)

Western blot - Anti-53BP2/ASPP2/BBP antibody (AB70548)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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