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Rabbit Recombinant Monoclonal 53BP2/ASPP2/BBP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (AB250500), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (AB250500), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (AB250500), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Expected
Tested
Not recommended
Mouse
Expected
Predicted
Not recommended
Rat
Expected
Predicted
Not recommended

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53 (PubMed:12524540). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of NAE1 to conjugate NEDD8 to CUL1, and thereby decreases NAE1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.

Alternative names

ASPP2, BBP, TP53BP2, Apoptosis-stimulating of p53 protein 2, Bcl2-binding protein, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, Bbp, 53BP2, p53-binding protein 2, p53BP2

Recommended products

Rabbit Recombinant Monoclonal 53BP2/ASPP2/BBP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR13837
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250500 is the carrier-free version of Anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

53BP2 also known as ASPP2 or BBP is a protein that interacts with the p53 tumor suppressor. It has a mass of approximately 140 kDa and is expressed in various tissues including the brain liver and lung. Mechanically 53BP2 modulates the activity of p53 influencing cellular apoptosis. The protein consists of several domains such as ankyrin repeats and SH3 domain that facilitate its interaction with multiple partners enhancing its regulatory function.

Biological function summary

53BP2 plays a role in apoptosis and cell cycle control. It acts as a co-activator in the p53 signaling pathway promoting the pro-apoptotic functions of p53. This protein forms part of a complex network involving p53 and other regulatory molecules which ultimately governs important cellular processes like growth arrest and death. By enhancing p53 transcriptional activity 53BP2 aids in maintaining cellular homeostasis and preventing uncontrolled cell proliferation.

Pathways

53BP2 is primarily involved in the p53 signaling and apoptosis pathways. It works closely with key proteins such as p53 and Bcl-2 enhancing the apoptotic response. In the p53 pathway 53BP2 strengthens p53's ability to transactivate genes essential for apoptosis while in the apoptotic pathway it interacts with members of the Bcl-2 family to regulate mitochondrial events leading to cell death.

Associated diseases and disorders

53BP2's function is relevant in cancer and neurodegenerative diseases. In cancer dysregulation of 53BP2 function or expression can lead to impaired apoptotic response contributing to tumorigenesis. It is linked to proteins like p53 and Bcl-2 in the context of cancer. In neurodegenerative diseases alterations in 53BP2 activity can affect neuronal cell survival with potential connections to proteins involved in synaptic pathways and apoptotic regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (ab250500), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (ab250500)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling 53BP2/ASPP2/BBP with purified Anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377 at 1/1000 dilution (0.15 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (ab250500), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (ab250500)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling 53BP2/ASPP2/BBP with purified Anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377 at 1/1000 dilution (0.15 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (ab250500), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] - BSA and Azide free (ab250500)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling 53BP2/ASPP2/BBP with purified Anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377 at 1/1000 dilution (0.15 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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