Goat Polyclonal 58K Golgi protein antibody. Golgi marker. Suitable for WB, Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human FTCD aa 1-50.
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Publications
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- Nucleic acids research 49:8277-82932021Golgi-58K can re-localize to late endosomes upon cellular uptake of PS-ASOs and facilitates endosomal release of ASOs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Hai Liang,Joshua G Nichols,Cheryl Li De Hoyos,Hong Sun,Lingdi Zhang,Stanley T CrookePubMed 34244781
- Journal of molecular neuroscience : MN 61:199-2142016The Novel Membrane-Bound Proteins MFSD1 and MFSD3 are Putative SLC Transporters Affected by Altered Nutrient Intake.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmelie Perland,Sofie V Hellsten,Emilia Lekholm,Mikaela M Eriksson,Vasiliki Arapi,Robert FredrikssonPubMed 27981419
- Scientific reports 5:149182015Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA).
- Protein expression and purification 74:16-232010Purification and characterization of Yersinia enterocolitica and Yersinia pestis LcrV-cholera toxin A(2)/B chimeras.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliette K Tinker et. al.PubMed 20438844
- The American journal of pathology 174:979-882009Aberrant heparan sulfate proteoglycan localization, despite normal exostosin, in central chondrosarcoma.
- The Journal of biological chemistry 283:27179-882008ADP-ribosylation factor-like GTPase ARFRP1 is required for trans-Golgi to plasma membrane trafficking of E-cadherin.
- Histopathology 52:465-742008Myxoid tumours of soft tissue: the so-called myxoid extracellular matrix is heterogeneous in composition.Applications:IHC-PReactive species:HumanS M Willems,Y M Schrage,J J Baelde,I Briaire-de Bruijn,A Mohseny,R Sciot,J V M G Bovée,P C W HogendoornPubMed 18315599
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FTCD aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95954
Reactivity data
Select an application| WB | Flow Cyt | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.03000-0.20000 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Binds and promotes bundling of vimentin filaments originating from the Golgi.
Alternative names
Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, LCHC1, FTCD
Recommended products
Goat Polyclonal 58K Golgi protein antibody. Golgi marker. Suitable for WB, Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human FTCD aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FTCD aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95954
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 58K Golgi protein also known as Golgi marker protein is a significant component within the Golgi apparatus with a molecular mass of approximately 58 kilodaltons (kDa). This protein is widely expressed in eukaryotic cells and serves as an important marker for studying the structure and function of the Golgi apparatus. The 58K Golgi protein localizes predominantly to the membranes of the Golgi where it plays an integral role in the maintenance of its architecture and function. Researchers often use this protein in Golgi immunofluorescence studies to visualize and track Golgi dynamics and morphology.
- Biological function summary
The 58K Golgi protein contributes to the Golgi's role in modifying sorting and packaging proteins and lipids for secretion or delivery to other organelles. It does not function alone but often interacts with other Golgi markers and proteins forming part of a complex that helps regulate intracellular transport processes. These interactions are important for maintaining Golgi function and cellular protein distribution. By acting as a Golgi biomarker the 58K Golgi protein aids in understanding the trafficking and processing pathways essential for cellular function.
- Pathways
The 58K Golgi protein is involved in the vesicular transport pathway which is essential for trafficking proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. It interacts with other proteins like COPI and COPII which are critical for vesicle formation and movement through this pathway. This protein also plays a role in the glycosylation pathway where nascent proteins undergo carbohydrate modification in the Golgi. Such modifications assist in protein folding stability and cell signaling. The integration of 58K Golgi protein in these pathways highlights its importance in sustaining cellular operations.
- Associated diseases and disorders
The 58K Golgi protein has correlations with some pathologies associated with Golgi dysfunction including congenital disorders of glycosylation and neurodegenerative diseases such as Alzheimer's disease. Disruptions in Golgi structure and function can lead to improper protein sorting and trafficking which is observed in these disorders. Proteins such as amyloid precursor protein (APP) in Alzheimer's are impacted by disruptions in the Golgi pathway where 58K Golgi protein plays a role. Understanding these connections can help in developing therapeutic strategies targeting Golgi-associated dysfunctions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-58K Golgi protein antibody - Golgi Marker (ab19072)
All lanes: Western blot - Anti-58K Golgi protein antibody - Golgi Marker (ab19072) at 0.05 µg/mL
All lanes: Human liver lysate at 35 µg
Predicted band size: 58 kDa
Flow Cytometry - Anti-58K Golgi protein antibody - Golgi Marker (ab19072)
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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