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AB19072

Anti-58K Golgi protein antibody - Golgi Marker

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(8 Publications)

Goat Polyclonal 58K Golgi protein antibody. Golgi marker. Suitable for WB, Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human FTCD aa 1-50.

View Alternative Names

Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, LCHC1, FTCD

2 Images
Flow Cytometry - Anti-58K Golgi protein antibody - Golgi Marker (AB19072)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-58K Golgi protein antibody - Golgi Marker (AB19072)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western blot - Anti-58K Golgi protein antibody - Golgi Marker (AB19072)
  • WB

Supplier Data

Western blot - Anti-58K Golgi protein antibody - Golgi Marker (AB19072)

All lanes:

Western blot - Anti-58K Golgi protein antibody - Golgi Marker (ab19072) at 0.05 µg/mL

All lanes:

Human liver lysate at 35 µg

Predicted band size: 58 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human FTCD aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O95954

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 58K Golgi protein also known as Golgi marker protein is a significant component within the Golgi apparatus with a molecular mass of approximately 58 kilodaltons (kDa). This protein is widely expressed in eukaryotic cells and serves as an important marker for studying the structure and function of the Golgi apparatus. The 58K Golgi protein localizes predominantly to the membranes of the Golgi where it plays an integral role in the maintenance of its architecture and function. Researchers often use this protein in Golgi immunofluorescence studies to visualize and track Golgi dynamics and morphology.
Biological function summary

The 58K Golgi protein contributes to the Golgi's role in modifying sorting and packaging proteins and lipids for secretion or delivery to other organelles. It does not function alone but often interacts with other Golgi markers and proteins forming part of a complex that helps regulate intracellular transport processes. These interactions are important for maintaining Golgi function and cellular protein distribution. By acting as a Golgi biomarker the 58K Golgi protein aids in understanding the trafficking and processing pathways essential for cellular function.

Pathways

The 58K Golgi protein is involved in the vesicular transport pathway which is essential for trafficking proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. It interacts with other proteins like COPI and COPII which are critical for vesicle formation and movement through this pathway. This protein also plays a role in the glycosylation pathway where nascent proteins undergo carbohydrate modification in the Golgi. Such modifications assist in protein folding stability and cell signaling. The integration of 58K Golgi protein in these pathways highlights its importance in sustaining cellular operations.

The 58K Golgi protein has correlations with some pathologies associated with Golgi dysfunction including congenital disorders of glycosylation and neurodegenerative diseases such as Alzheimer's disease. Disruptions in Golgi structure and function can lead to improper protein sorting and trafficking which is observed in these disorders. Proteins such as amyloid precursor protein (APP) in Alzheimer's are impacted by disruptions in the Golgi pathway where 58K Golgi protein plays a role. Understanding these connections can help in developing therapeutic strategies targeting Golgi-associated dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.. Binds and promotes bundling of vimentin filaments originating from the Golgi.
See full target information FTCD
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 21:e13584 PubMed35318803

2022

Acidic nanoparticles protect against α-synuclein-induced neurodegeneration through the restoration of lysosomal function.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Laure Arotcarena,Federico N Soria,Anthony Cunha,Evelyne Doudnikoff,Geoffrey Prévot,Jonathan Daniel,Mireille Blanchard-Desce,Philippe Barthélémy,Erwan Bezard,Sylvie Crauste-Manciet,Benjamin Dehay

Nucleic acids research 49:8277-8293 PubMed34244781

2021

Golgi-58K can re-localize to late endosomes upon cellular uptake of PS-ASOs and facilitates endosomal release of ASOs.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Hai Liang,Joshua G Nichols,Cheryl Li De Hoyos,Hong Sun,Lingdi Zhang,Stanley T Crooke

Journal of molecular neuroscience : MN 61:199-214 PubMed27981419

2016

The Novel Membrane-Bound Proteins MFSD1 and MFSD3 are Putative SLC Transporters Affected by Altered Nutrient Intake.

Applications

Unspecified application

Species

Unspecified reactive species

Emelie Perland,Sofie V Hellsten,Emilia Lekholm,Mikaela M Eriksson,Vasiliki Arapi,Robert Fredriksson

Scientific reports 5:14918 PubMed26446566

2015

Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA).

Applications

WB

Species

Unspecified reactive species

Huinan Li,Cheng Wu,Rodolfo Aramayo,Matthew S Sachs,Mark L Harlow

Protein expression and purification 74:16-23 PubMed20438844

2010

Purification and characterization of Yersinia enterocolitica and Yersinia pestis LcrV-cholera toxin A(2)/B chimeras.

Applications

Unspecified application

Species

Unspecified reactive species

Juliette K Tinker,Chadwick T Davis,Britni M Arlian

The American journal of pathology 174:979-88 PubMed19179614

2009

Aberrant heparan sulfate proteoglycan localization, despite normal exostosin, in central chondrosarcoma.

Applications

IHC-P

Species

Human

Yvonne M Schrage,Liesbeth Hameetman,Karoly Szuhai,Anne-Marie Cleton-Jansen,Antonie H M Taminiau,Pancras C W Hogendoorn,Judith V M G Bovée

The Journal of biological chemistry 283:27179-88 PubMed18662990

2008

ADP-ribosylation factor-like GTPase ARFRP1 is required for trans-Golgi to plasma membrane trafficking of E-cadherin.

Applications

IHC

Species

Mouse

Claudia Zahn,Alexander Jaschke,Jörg Weiske,Angela Hommel,Deike Hesse,Robert Augustin,Lei Lu,Wanjin Hong,Simone Florian,Andrea Scheepers,Hans-Georg Joost,Otmar Huber,Annette Schürmann

Histopathology 52:465-74 PubMed18315599

2008

Myxoid tumours of soft tissue: the so-called myxoid extracellular matrix is heterogeneous in composition.

Applications

IHC-P

Species

Human

S M Willems,Y M Schrage,J J Baelde,I Briaire-de Bruijn,A Mohseny,R Sciot,J V M G Bovée,P C W Hogendoorn
View all publications
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