AB227165

Anti-5HT1A Receptor antibody

Name: Anti-5HT1A Receptor antibody (ab227165) | Abcam
Brand: Abcam Limited
SKU: AB227165

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(8 Publications )

Rabbit Polyclonal 5HT1A Receptor antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human HTR1A.

View Alternative Names

ADRB2RL1, ADRBRL1, HTR1A, 5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, G-21, Serotonin receptor 1A

2 Images

Supplier Data

Western blot - Anti-5HT1A Receptor antibody (AB227165)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-5HT1A Receptor antibody (ab227165) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-5HT1A Receptor antibody (AB227165)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-5HT1A Receptor antibody (ab227165) at 1/1000 dilution

All lanes:

D-hippocampus C9 lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human HTR1A. The exact immunogen used to generate this antibody is proprietary information.

P08908

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5HT1A receptor also known as 5-HT1A or serotonin receptor 1A is part of the 5-HT receptor family. This receptor functions mechanically as a G protein-coupled receptor (GPCR) with a molecular mass of approximately 46 kDa. It is widely expressed in the brain with high concentrations in areas like the hippocampus raphe nuclei and cerebral cortex. Additionally you can find 5HT1A receptors in peripheral tissues although at lower levels. They play an essential role in mediating inhibitory neurotransmission by reducing the production of cAMP.

Biological function summary

The 5HT1A receptor is important in regulating mood anxiety and cognition. These receptors function as part of a receptor complex interacting with other serotonin receptors like 5-HT2 and 5-HT3 to modulate neural processes. The 5HT1A receptor mediates its effects by inhibiting adenylate cyclase resulting in decreased levels of intracellular cAMP. This inhibition subsequently affects ion channel function and neurotransmitter release influencing various mental and physiological processes.

Pathways

Several serotonin pathways involve the 5HT1A receptor including the serotonergic and dopaminergic pathways. These pathways are essential for healthy brain function and mood regulation. The receptor also interacts with the protein RGS10 which modulates G protein signaling. RGS10 can influence the pathways the 5HT1A receptor is involved in providing a regulatory mechanism for neurotransmission and receptor activity.

The 5HT1A receptor is linked to depression and anxiety disorders. Abnormalities in the function or expression of this receptor can result in altered serotonergic signaling contributing to these conditions. The receptor's relationship with the protein serotonergic 5-HT2A receptor can modulate the risk and progression of mood disorders. Researchers are actively investigating potential therapies that target the 5-HT1A receptor to alleviate symptoms of these mental health conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 22957663, PubMed : 3138543, PubMed : 33762731, PubMed : 37935376, PubMed : 37935377, PubMed : 8138923, PubMed : 8393041). Also functions as a receptor for various drugs and psychoactive substances (PubMed : 22957663, PubMed : 3138543, PubMed : 33762731, PubMed : 38552625, PubMed : 8138923, PubMed : 8393041). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 22957663, PubMed : 3138543, PubMed : 33762731, PubMed : 8138923, PubMed : 8393041). HTR1A is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission : signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores (PubMed : 33762731, PubMed : 35610220). Beta-arrestin family members regulate signaling by mediating both receptor desensitization and resensitization processes (PubMed : 18476671, PubMed : 20363322, PubMed : 20945968). Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism (PubMed : 18476671, PubMed : 20363322, PubMed : 20945968). Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior (PubMed : 18476671, PubMed : 20363322, PubMed : 20945968). Plays a role in the response to anxiogenic stimuli (PubMed : 18476671, PubMed : 20363322, PubMed : 20945968).

See full target information HTR1A

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 148:76 PubMed39607506

2024

Raphe and ventrolateral medulla proteomics in sudden unexplained death in childhood with febrile seizure history.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique F Leitner,Christopher William,Arline Faustin,Evgeny Kanshin,Matija Snuderl,Declan McGuone,Thomas Wisniewski,Beatrix Ueberheide,Laura Gould,Orrin Devinsky

Neurobiology of disease 183:106179 PubMed37276987

2023

Reduction of aggressive behaviour following hypothalamic deep brain stimulation: Involvement of 5-HT and testosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Venetucci Gouveia,Mustansir Diwan,Raquel C R Martinez,Peter Giacobbe,Nir Lipsman,Clement Hamani

International journal of molecular sciences 24: PubMed36769290

2023

Expression Patterns of Serotonin Receptors 5-HT1A, 5-HT2A, and 5-HT3A during Human Fetal Lung Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jelena Nikolić,Katarina Vukojević,Violeta Šoljić,Josip Mišković,Martina Orlović Vlaho,Mirna Saraga-Babić,Natalija Filipović

Scientific reports 12:17973 PubMed36289270

2022

Environmentally-relevant concentrations of the antipsychotic drugs sulpiride and clozapine induce abnormal dopamine and serotonin signaling in zebrafish brain.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Xijian Peng,Xiumei Sun,Yuanming Guo,Tiejun Li

Epilepsia 63:2925-2936 PubMed36053862

2022

Serotonin receptor expression in hippocampus and temporal cortex of temporal lobe epilepsy patients by postictal generalized electroencephalographic suppression duration.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique Frances Leitner,Sasha Devore,Juliana Laze,Daniel Friedman,James D Mills,Yan Liu,Michael Janitz,Jasper J Anink,Johannes C Baayen,Sander Idema,Erwin Alexander van Vliet,Beate Diehl,Catherine Scott,Roland Thijs,Maromi Nei,Manor Askenazi,Shobi Sivathamboo,Terence O'Brien,Thomas Wisniewski,Maria Thom,Eleonora Aronica,Maura Boldrini,Orrin Devinsky

International journal of molecular sciences 22: PubMed34298938

2021

Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida.

Applications

Unspecified application

Species

Unspecified reactive species

Hrvoje Punda,Snjezana Mardesic,Natalija Filipovic,Ivona Kosovic,Benjamin Benzon,Marin Ogorevc,Ivana Bocina,Kresimir Kolic,Katarina Vukojevic,Mirna Saraga-Babic

Comprehensive psychoneuroendocrinology 7:100063 PubMed35757063

2021

Adolescent use of potential novel probiotic subsp. acadiensis () mitigates pubertal LPS-Induced behavioral changes in adulthood in a sex-specific manner by modulating 5HT1A receptors expression in specific brain areas.

Applications

Unspecified application

Species

Unspecified reactive species

Nour Yahfoufi,Emily G Ah-Yen,Rajini Chandrasegaram,Sarah Aly,Michael Murack,Anthony K Kadamani,Chantal Matar,Nafissa Ismail

Journal of dental research 98:1262-1270 PubMed31454276

2019

Axin2-Mesenchymal PDL Cells, Instead of K14 Epithelial Cells, Play a Key Role in Rapid Cementum Growth.

Applications

Unspecified application

Species

Unspecified reactive species

X Xie,J Wang,K Wang,C Li,S Zhang,D Jing,C Xu,X Wang,H Zhao,J Q Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-5HT1A Receptor antibody

Western blot - Anti-5HT1A Receptor antibody (AB227165)

Western blot - Anti-5HT1A Receptor antibody (AB227165)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise