Rabbit Recombinant Monoclonal 5HT1A Receptor antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (AB283351), expandable thumbnail](https://content.abcam.com/products/images/5ht1a-receptor-antibody-epr24117-4-bsa-and-azide-free-ab283351--immunocytochemistry-immunofluorescence-img177744.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (AB283351)")
![Flow Cytometry (Intracellular) - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (AB283351), expandable thumbnail](https://content.abcam.com/products/images/5ht1a-receptor-antibody-epr24117-4-bsa-and-azide-free-ab283351--flow-cytometry-intracellular-img173521.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (AB283351)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt (Intra) | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:2140514, PubMed:2156831). Also functions as a receptor for various drugs and psychoactive substances (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (By similarity). HTR1A is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission: signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores (By similarity). Beta-arrestin family members regulate signaling by mediating both receptor desensitization and resensitization processes (By similarity).
Alternative names
5ht1a, Htr1a, 5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, Serotonin receptor 1A
Recommended products
Rabbit Recombinant Monoclonal 5HT1A Receptor antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24117-4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab283351 is the carrier-free version of Anti-5HT1A Receptor antibody [EPR24117-4] ab259896.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 5HT1A receptor also known as 5-HT1A or serotonin receptor 1A is part of the 5-HT receptor family. This receptor functions mechanically as a G protein-coupled receptor (GPCR) with a molecular mass of approximately 46 kDa. It is widely expressed in the brain with high concentrations in areas like the hippocampus raphe nuclei and cerebral cortex. Additionally you can find 5HT1A receptors in peripheral tissues although at lower levels. They play an essential role in mediating inhibitory neurotransmission by reducing the production of cAMP.
- Biological function summary
The 5HT1A receptor is important in regulating mood anxiety and cognition. These receptors function as part of a receptor complex interacting with other serotonin receptors like 5-HT2 and 5-HT3 to modulate neural processes. The 5HT1A receptor mediates its effects by inhibiting adenylate cyclase resulting in decreased levels of intracellular cAMP. This inhibition subsequently affects ion channel function and neurotransmitter release influencing various mental and physiological processes.
- Pathways
Several serotonin pathways involve the 5HT1A receptor including the serotonergic and dopaminergic pathways. These pathways are essential for healthy brain function and mood regulation. The receptor also interacts with the protein RGS10 which modulates G protein signaling. RGS10 can influence the pathways the 5HT1A receptor is involved in providing a regulatory mechanism for neurotransmission and receptor activity.
- Associated diseases and disorders
The 5HT1A receptor is linked to depression and anxiety disorders. Abnormalities in the function or expression of this receptor can result in altered serotonergic signaling contributing to these conditions. The receptor's relationship with the protein serotonergic 5-HT2A receptor can modulate the risk and progression of mood disorders. Researchers are actively investigating potential therapies that target the 5-HT1A receptor to alleviate symptoms of these mental health conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (ab283351), expandable thumbnail](https://content.abcam.com/products/images/5ht1a-receptor-antibody-epr24117-4-bsa-and-azide-free-ab283351--immunocytochemistry-immunofluorescence-img177744.jpg "Immunocytochemistry/ Immunofluorescence - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (ab283351)")
Immunocytochemistry/ Immunofluorescence - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (ab283351)
This data was developed using Anti-5HT1A Receptor antibody [EPR24117-4] ab259896, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling 5HT1A Receptor with Anti-5HT1A Receptor antibody [EPR24117-4] ab259896 at 1/250 (2.316 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged Htr1a expression vector is observed. Alexa Fluor® 488 Anti-Myc tag antibody [9E10] ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 4ug/ml dilution.
![Flow Cytometry (Intracellular) - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (ab283351), expandable thumbnail](https://content.abcam.com/products/images/5ht1a-receptor-antibody-epr24117-4-bsa-and-azide-free-ab283351--flow-cytometry-intracellular-img173521.jpg "Flow Cytometry (Intracellular) - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (ab283351)")
Flow Cytometry (Intracellular) - Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free (ab283351)
This data was developed using Anti-5HT1A Receptor antibody [EPR24117-4] ab259896, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with myc tagged 5HT1A receptor construct cells labelling 5HT1A Receptor with Anti-5HT1A Receptor antibody [EPR24117-4] ab259896 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
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