Skip to main content

Rabbit Polyclonal 5HT1B Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.


Images

Western blot - Anti-5HT1B Receptor antibody (AB85937), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Chimpanzee
Predicted
Common marmoset
Predicted
Gorilla
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Chimpanzee, Gorilla, Common marmoset
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:10452531, PubMed:1315531, PubMed:1328844, PubMed:1348246, PubMed:1351684, PubMed:1559993, PubMed:1565658, PubMed:1610347, PubMed:23519210, PubMed:23519215, PubMed:29925951, PubMed:8218242). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD) (PubMed:23519210, PubMed:23519215, PubMed:29925951). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:10452531, PubMed:1315531, PubMed:1328844, PubMed:1348246, PubMed:1351684, PubMed:1559993, PubMed:1565658, PubMed:1610347, PubMed:23519210, PubMed:23519215, PubMed:29925951, PubMed:8218242). HTR1B is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission by inhibiting adenylate cyclase activity (PubMed:29925951, PubMed:35610220). Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed:29925951). Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior (PubMed:18476671, PubMed:20945968). Besides, plays a role in vasoconstriction of cerebral arteries (PubMed:15853772).

Alternative names

HTR1DB, HTR1B, 5-hydroxytryptamine receptor 1B, 5-HT-1B, 5-HT1B, S12, Serotonin 1D beta receptor, Serotonin receptor 1B, 5-HT-1D-beta

Recommended products

Rabbit Polyclonal 5HT1B Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Specificity

This antibody has been sucessfully used on rat tissue in IHC. Since the immunogen only shares 84% homology, customers may get variable results. Please see the abreview for more details.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The 5-HT1B receptor also known as the serotonin 1B receptor or HTR1B is a G protein-coupled receptor with a molecular mass of approximately 41 kDa. It binds serotonin an important neurotransmitter in the central nervous system. The 5-HT1B receptor is expressed in various brain regions including the striatum basal ganglia and frontal cortex. This receptor modulates neurotransmission by inhibiting the release of serotonin and other neurotransmitters like dopamine. Interruptions in its function can significantly affect synaptic plasticity and neuronal activity.

Biological function summary

The 5-HT1B receptor plays a role in regulating aggressive behavior mood and appetite. It is part of a larger serotonin receptor family and works in conjunction with other receptor subtypes to maintain effective serotonergic signaling. By influencing dopamine and GABAergic systems the 5-HT1B receptor contributes to neural inhibition and the modulation of reward pathways. Alterations in its activity could influence emotional regulation and various behavioral responses.

Pathways

The 5-HT1B receptor participates significantly in the serotonin signaling pathway and MAPK signaling pathway. Interacting with proteins such as G protein-coupled receptor kinases and beta-arrestins it influences downstream effects that include modulation of cAMP levels and changes in phosphatidylinositol turnover. Disturbances in these pathways can affect mood and behavior implicating the 5-HT1B receptor in various neurological pathways.

Associated diseases and disorders

Irregular function of the 5-HT1B receptor links to conditions such as depression and migraine. This receptor through its role in mood regulation implicates it in mood disorders if dysregulated. It also connects to genetic factors influencing migraine possibly through interactions with proteins like calcitonin gene-related peptide (CGRP). Despite the complexity of these conditions the 5-HT1B receptor is a potential target for therapeutic interventions suggesting its importance in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-5HT1B Receptor antibody (ab85937), expandable thumbnail

    Western blot - Anti-5HT1B Receptor antibody (ab85937)

    All lanes: 5HT1B Receptor antibody at 1 µg/mL

    All lanes: Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa

    Observed band size: 43 kDa

    Exposure time: 20min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com