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AB140486

Anti-5HT1D Receptor antibody

1

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal 5HT1D Receptor antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HTR1D.

View Alternative Names

HTR1DA, HTRL, HTR1D, 5-hydroxytryptamine receptor 1D, 5-HT-1D, 5-HT1D, Serotonin 1D alpha receptor, Serotonin receptor 1D, 5-HT-1D-alpha

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1D Receptor antibody (AB140486)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1D Receptor antibody (AB140486)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human spinal cord, dorsal root ganglion tissue labelling 5HT1D Receptor with ab140486 at 17 µg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human HTR1D. The exact immunogen used to generate this antibody is proprietary information.

P28221

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The '5HT1D Receptor' also known as the serotonin receptor 1D is a protein that plays an important role in neurotransmission. It is a G-protein coupled receptor (GPCR) with a molecular mass of approximately 47 kDa. This receptor is widely expressed in the human central nervous system particularly in the brain regions such as the basal ganglia and the frontal cortex. The 5HT1D receptor is involved in inhibiting the release of serotonin and other neurotransmitters acting as an auto-receptor in the serotonergic pathways.
Biological function summary

The 5HT1D receptor functions in the regulation of mood anxiety and vasoconstriction. It does not form part of a known receptor complex but interacts with intracellular signaling molecules to produce its effects. The receptor modulates the release of various neurotransmitters including dopamine and norepinephrine thereby influencing neurological processes and behaviors. Its role is critical in managing the serotonergic tone within different central nervous system components.

Pathways

The 5HT1D receptor is integrated into the serotonergic signaling pathway impacting both neurological and peripheral systems. It interacts with other serotonin receptors such as 5HT1B which also participate in controlling neurotransmitter release. The cAMP signaling pathway is another significant pathway where the receptor plays a part by inhibiting the production of cAMP ultimately affecting neuronal excitability and response.

The 5HT1D receptor is notably associated with migraines and depression. The receptor’s function in regulating serotonin release makes it a target for migraine treatments as alterations in serotonin levels are linked with migraine pathophysiology. In the context of depression 5HT1D receptors are studied for their potential role in the disorder's symptomatology and treatment often in connection with other proteins such as serotonin transporters (SERT) that influence serotonin availability in synaptic clefts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 10452531, PubMed : 1565658, PubMed : 1652050, PubMed : 33762731). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances (PubMed : 10452531, PubMed : 1565658, PubMed : 1652050, PubMed : 33762731). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 10452531, PubMed : 1565658, PubMed : 1652050, PubMed : 33762731). HTR1D is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission by inhibiting adenylate cyclase activity (PubMed : 33762731). Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity (PubMed : 18476671, PubMed : 20945968). May also play a role in regulating the release of other neurotransmitters (PubMed : 18476671, PubMed : 20945968). May play a role in vasoconstriction (PubMed : 18476671, PubMed : 20945968).
See full target information HTR1D
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioscience (Landmark edition) 28:1 PubMed36722266

2023

Mast Cell-Associated Serotonin in Acupoint Contributes to Acupuncture Analgesia in Arthritis Rats by Mediating ATP Release.

Applications

Unspecified application

Species

Unspecified reactive species

Bing-Rong Li,Yu-Jia Li,Jing-Wen Xu,Meng Huang,Li-Na Wang,Di Zhang,Xue-Yong Shen,Guang-Hong Ding

Cephalalgia : an international journal of headache 37:1350-1372 PubMed27852962

2016

Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine.

Applications

Unspecified application

Species

Unspecified reactive species

Frank L Rice,Jennifer Y Xie,Phillip J Albrecht,Emily Acker,Justin Bourgeois,Edita Navratilova,David W Dodick,Frank Porreca

PloS one 10:e0117581 PubMed25807145

2015

Discovery of molecular markers to discriminate corneal endothelial cells in the human body.

Applications

Unspecified application

Species

Unspecified reactive species

Masahito Yoshihara,Hiroko Ohmiya,Susumu Hara,Satoshi Kawasaki,Yoshihide Hayashizaki,Masayoshi Itoh,Hideya Kawaji,Motokazu Tsujikawa,Kohji Nishida
View all publications
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