AB140524

Anti-5HT2A Receptor antibody - C-terminal

Name: Anti-5HT2A Receptor antibody - C-terminal (ab140524) | Abcam
Brand: Abcam Limited
SKU: AB140524

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(4 Publications)

Rabbit Polyclonal 5HT2A Receptor antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human HTR2A.

View Alternative Names

HTR2, HTR2A, 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT2A Receptor antibody - C-terminal (AB140524)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT2A Receptor antibody - C-terminal (AB140524)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human adrenal tissue labelling 5HT2A Receptor with ab140524 at 10 µg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT2A Receptor antibody - C-terminal (AB140524)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human placenta tissue labelling 5HT2A Receptor with ab140524 at 10 µg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human HTR2A. The exact immunogen used to generate this antibody is proprietary information.

P28223

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5HT2A receptor also known as 5-hydroxytryptamine receptor 2A is a G-protein coupled receptor with a molecular mass of approximately 52 kDa. It is one of the key receptors for serotonin (5-HT) and is chiefly expressed in the central nervous system including regions like the cortex and the basal ganglia. This receptor binds serotonin and initiates a cascade of intracellular events which can alter neuronal activity and influence various neuropsychological functions.

Biological function summary

The 5HT2A receptor modulates cognitive functions such as learning and memory and it also plays a role in mood regulation. This receptor is involved in a complex of signaling pathways and interacts with other receptors such as the 5HT1A receptor and proteins like phospholipase C in downstream signaling. Through these interactions the receptor contributes to maintaining a balance in neurotransmitter systems.

Pathways

This receptor is actively involved in the serotonergic signaling pathway. It participates by interacting with the phosphatidylinositol signaling system affecting intracellular calcium levels. The 5HT2A receptor works alongside other proteins such as the dopamine D2 receptor forming a link between serotonin and dopamine pathways which are integral to mood and behavior regulation.

The 5HT2A receptor is closely linked to schizophrenia and depression. Alterations in the receptor's function or expression levels have been associated with the pathophysiology of these disorders. The receptor's interactions with other proteins including the 5HT1A receptor have been implicated in the therapeutic mechanisms of antipsychotic and antidepressant medications highlighting its significance in mental health treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 1330647, PubMed : 18703043, PubMed : 19057895, PubMed : 21645528, PubMed : 22300836, PubMed : 35084960, PubMed : 38552625). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (PubMed : 28129538, PubMed : 35084960). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 28129538, PubMed : 35084960). HTR2A is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 28129538, PubMed : 35084960). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 28129538, PubMed : 35084960). Affects neural activity, perception, cognition and mood (PubMed : 18297054). Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction (By similarity).. (Microbial infection) Acts as a receptor for human JC polyomavirus/JCPyV.

See full target information HTR2A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 22:e3002822 PubMed39352884

2024

Experience-dependent serotonergic signaling in glia regulates targeted synapse elimination.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Kay Miller,Kendal Broadie

Biochemical Society transactions 49:1997-2006 PubMed34397081

2021

How neural stem cells contribute to neocortex development.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Xing,Michaela Wilsch-Bräuninger,Wieland B Huttner

Neuron 108:1113-1129.e6 PubMed33080227

2020

Serotonin Receptor 2A Activation Promotes Evolutionarily Relevant Basal Progenitor Proliferation in the Developing Neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Xing,Nereo Kalebic,Takashi Namba,Samir Vaid,Pauline Wimberger,Wieland B Huttner

Scientific reports 7:15453 PubMed29133847

2017

Female mice lacking Pald1 exhibit endothelial cell apoptosis and emphysema.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Egaña,Hiroshi Kaito,Anja Nitzsche,Lore Becker,Carolina Ballester-Lopez,Colin Niaudet,Milena Petkova,Wei Liu,Michael Vanlandewijck,Alexandra Vernaleken,Thomas Klopstock,Helmut Fuchs,Valerie Gailus-Durner,Martin Hrabe de Angelis,Helge Rask-Andersen,Henrik J Johansson,Janne Lehtiö,Liqun He,Ali Ö Yildirim,Mats Hellström

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-5HT2A Receptor antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT2A Receptor antibody - C-terminal (AB140524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT2A Receptor antibody - C-terminal (AB140524)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise