AB13897

Anti-5HT3A receptor/5ht3 antibody

Name: Anti-5HT3A receptor/5ht3 antibody. Rabbit polyclonal (ab13897) | Abcam
Brand: Abcam Limited
SKU: AB13897
Price: 535 USD
Availability: InStock
Rating: 4 (8 reviews)

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(8 Reviews)

(15 Publications)

Rabbit Polyclonal 5HT3A receptor/5ht3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Rat Htr3a.

View Alternative Names

5HT3R, HTR3, HTR3A, 5-hydroxytryptamine receptor 3A, 5-HT3-A, 5-HT3A, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor, 5-HT-3, 5-HT3R

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (AB13897)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (AB13897)

IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Western blot - Anti-5HT3A receptor/5ht3 antibody (AB13897)

Western Blot of human brain lysate staining 5HT3A receptor with ab13897

All lanes:

Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)

Predicted band size: 55 kDa

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Western blot - Anti-5HT3A receptor/5ht3 antibody (AB13897)

All lanes:

Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897) at 3 µg/mL

Lane 1:

Human Brain tissue lysate

Lane 2:

Mouse Brain tissue lysate

Lane 3:

Rat Brain tissue lysate

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Rat Htr3a. The exact immunogen used to generate this antibody is proprietary information.

P35563

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5HT3A receptor also known as the 5-HT3 receptor or 5HT3 forms part of the serotonin receptor family. These ligand-gated ion channels enable the rapid conduction of cations such as Na+ and Ca2+ into cells upon activation. The 5-HT3A receptor exhibits a molecular weight of approximately 55 to 60 kDa and is expressed broadly in peripheral and central nervous systems including regions like the cortex hippocampus and gastrointestinal tract. This expression pattern allows it to perform roles in neurotransmission and modulate physiological responses across different systems.

Biological function summary

The 5-HT3A receptor acts as a critical mediator of synaptic signalling and neurotransmitter release offering a path for serotonin's effects. As part of a pentameric complex 5-HT3A subunits combine to form functional ion channels. The receptor particularly influences excitatory neurotransmission affecting processes involved in mood regulation nausea and vomiting. Consequently this receptor serves key physiological functions through its modulation of ionic flux and neuronal activity.

Pathways

The 5-HT3A receptor plays an essential role in pathways related to neurotransmission and neuroplasticity. Within serotonin signalling pathways 5HT3A closely associates with proteins such as serotonin transporter (SERT) elaborating on its role in serotonin uptake and homeostasis. Furthermore its participation in these pathways links to the regulation of calcium-dependent processes interacting with calmodulins and other calcium-binding proteins to influence downstream signalling events essential for cognitive processes and sensory signalling.

The 5-HT3A receptor has been associated with conditions like anxiety and irritable bowel syndrome (IBS). In anxiety disorders its role in modulating serotonin-induced neurotransmission points to its involvement in mood disorders where altered serotonin balance is a contributing factor. As for IBS the receptor's high expression in the gut highlights its influence on gastrointestinal motility and secretion. Researchers also explore its connection with GABAergic systems pointing to potential therapeutic targets for managing symptoms in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.

See full target information HTR3A

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

World journal of psychiatry 15:105751 PubMed40574756

2025

Increased expression of the neuroplastin 65 protein is involved in neurofibrillary tangles and amyloid beta plaques in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ni Zheng,Yan Wang,Ling Chen,Li-Zhang Xu,Lei Zhang,Jia-Lu Wang,Juan Liu,Qi-Lei Zhang,Qiong-Lan Yuan

Acta pharmacologica Sinica 45:1393-1405 PubMed38528118

2024

Disfunction of dorsal raphe nucleus-hippocampus serotonergic-HTR3 transmission results in anxiety phenotype of Neuroplastin 65-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Cheng,Ling Chen,Ya-Ni Zheng,Juan Liu,Lei Zhang,Xiao-Ming Zhang,Liang Huang,Qiong-Lan Yuan

Histology and histopathology 39:903-919 PubMed38108436

2023

Differential roles of serotonin receptor subtypes in regulation of neurotrophin receptor expression and intestinal hypernociception.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ping She,Yu-Ting Hsieh,Li-Yu Lin,Chia-Hung Tu,Ming-Shiang Wu,Ling-Wei Hsin,Linda Chia-Hui Yu

Life (Basel, Switzerland) 12: PubMed36295079

2022

Serotonin Type 3 Receptor Is Potentially Involved in Cellular Stress Induced by Hydrogen Peroxide.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Salomé Correia,Isabel Silva,José Carlos Oliveira,Henrique Reguengo,Nuno Vale

Nature communications 13:2901 PubMed35614045

2022

Serotonin limits generation of chromaffin cells during adrenal organ development.

Applications

Unspecified application

Species

Unspecified reactive species

Polina Kameneva,Victoria I Melnikova,Maria Eleni Kastriti,Anastasia Kurtova,Emil Kryukov,Aliia Murtazina,Louis Faure,Irina Poverennaya,Artem V Artemov,Tatiana S Kalinina,Nikita V Kudryashov,Michael Bader,Jan Skoda,Petr Chlapek,Lucie Curylova,Lukas Sourada,Jakub Neradil,Marketa Tesarova,Massimo Pasqualetti,Patricia Gaspar,Vasily D Yakushov,Boris I Sheftel,Tomas Zikmund,Jozef Kaiser,Kaj Fried,Natalia Alenina,Elena E Voronezhskaya,Igor Adameyko

Laboratory investigation; a journal of technical methods and pathology 102:1023-1037 PubMed35585132

2022

5-HT receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Ying Chang,Yi-Ting Yang,Meng-Ping She,Chia-Hung Tu,Tsung-Chun Lee,Ming-Shiang Wu,Chin-Hung Sun,Ling-Wei Hsin,Linda Chia-Hui Yu

Drug design, development and therapy 14:4085-4099 PubMed33061309

2020

[6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT Receptor System in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Cheng,Xiaodi Feng,Qi Meng,Yaqi Li,Siqi Chen,Guoen Wang,Ke Nie

Molecular medicine reports 22:763-773 PubMed32626986

2020

Effects of sacral nerve electrical stimulation on 5‑HT and 5‑HT3AR/5‑HT4R levels in the colon and sacral cord of acute spinal cord injury rat models.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhu,Jie Cheng,Jichao Yin,Yujie Yang,Jiabao Guo,Wenyi Zhang,Bing Xie,Haixia Lu,Dingjun Hao

FASEB journal : official publication of the Federa 33:13560-13571 PubMed31570003

2019

The comprehensive pathophysiological changes in a novel rat model of postinflammatory visceral hypersensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Jianhui Li,Meirui Qian,Nan He,Ying Cao,Yaping Liu,Kaichun Wu,Shuixiang He

FASEB journal : official publication of the Federa 33:10731-10741 PubMed31251077

2019

Prolongation of bronchopulmonary C-fiber-mediated apnea by prenatal nicotinic exposure in rat pups: role of 5-HT receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhao,Xiuping Gao,Jianguo Zhuang,Morgan Wallen,Shuguang Leng,Fadi Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-5HT3A receptor/5ht3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (AB13897)

Western blot - Anti-5HT3A receptor/5ht3 antibody (AB13897)

Western blot - Anti-5HT3A receptor/5ht3 antibody (AB13897)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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