Rabbit Polyclonal 5HT3A receptor/5ht3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Rat Htr3a.
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Publications
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- Histology and histopathology 39:903-9192023Differential roles of serotonin receptor subtypes in regulation of neurotrophin receptor expression and intestinal hypernociception.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Ping She,Yu-Ting Hsieh,Li-Yu Lin,Chia-Hung Tu,Ming-Shiang Wu,Ling-Wei Hsin,Linda Chia-Hui YuPubMed 38108436
- Life (Basel, Switzerland) 12:2022Serotonin Type 3 Receptor Is Potentially Involved in Cellular Stress Induced by Hydrogen Peroxide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Salomé Correia,Isabel Silva,José Carlos Oliveira,Henrique Reguengo,Nuno ValePubMed 36295079
- Nature communications 13:29012022Serotonin limits generation of chromaffin cells during adrenal organ development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPolina Kameneva,Victoria I Melnikova,Maria Eleni Kastriti,Anastasia Kurtova,Emil Kryukov,Aliia Murtazina,Louis Faure,Irina Poverennaya,Artem V Artemov,Tatiana S Kalinina,Nikita V Kudryashov,Michael Bader,Jan Skoda,Petr Chlapek,Lucie Curylova,Lukas Sourada,Jakub Neradil,Marketa Tesarova,Massimo Pasqualetti,Patricia Gaspar,Vasily D Yakushov,Boris I Sheftel,Tomas Zikmund,Jozef Kaiser,Kaj Fried,Natalia Alenina,Elena E Voronezhskaya,Igor AdameykoPubMed 35614045
- Laboratory investigation; a journal of technical methods and pathology 102:1023-103720225-HT receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Ying Chang,Yi-Ting Yang,Meng-Ping She,Chia-Hung Tu,Tsung-Chun Lee,Ming-Shiang Wu,Chin-Hung Sun,Ling-Wei Hsin,Linda Chia-Hui YuPubMed 35585132
- Drug design, development and therapy 14:4085-40992020[6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT Receptor System in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianqian Cheng et. al.PubMed 33061309
- Molecular medicine reports 22:763-7732020Effects of sacral nerve electrical stimulation on 5‑HT and 5‑HT3AR/5‑HT4R levels in the colon and sacral cord of acute spinal cord injury rat models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhu et. al.PubMed 32626986
- FASEB journal : official publication of the Federa 33:10731-107412019Prolongation of bronchopulmonary C-fiber-mediated apnea by prenatal nicotinic exposure in rat pups: role of 5-HT receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhao et. al.PubMed 31251077
- Phytomedicine : international journal of phytother 63:1529822019Deciphering the multicomponent synergy mechanisms of SiNiSan prescription on irritable bowel syndrome using a bioinformatics/network topology based strategy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBangjie Li et. al.PubMed 31299593
- Biochemical and biophysical research communication 511:356-3622019LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Lin Fu et. al.PubMed 30791981
- Neurogastroenterology and motility 20:228-352007Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Htr3a. The exact immunogen used to generate this antibody is proprietary information. Database link P35563
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes - |
Species Mouse | Dilution info 1.00000-3.00000 µg/mL | Notes - |
Species Rat | Dilution info 1.00000-3.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.
Alternative names
5HT3R, HTR3, HTR3A, 5-hydroxytryptamine receptor 3A, 5-HT3-A, 5-HT3A, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor, 5-HT-3, 5-HT3R
Recommended products
Rabbit Polyclonal 5HT3A receptor/5ht3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Rat Htr3a.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Htr3a. The exact immunogen used to generate this antibody is proprietary information. Database link P35563
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 5HT3A receptor also known as the 5-HT3 receptor or 5HT3 forms part of the serotonin receptor family. These ligand-gated ion channels enable the rapid conduction of cations such as Na+ and Ca2+ into cells upon activation. The 5-HT3A receptor exhibits a molecular weight of approximately 55 to 60 kDa and is expressed broadly in peripheral and central nervous systems including regions like the cortex hippocampus and gastrointestinal tract. This expression pattern allows it to perform roles in neurotransmission and modulate physiological responses across different systems.
- Biological function summary
The 5-HT3A receptor acts as a critical mediator of synaptic signalling and neurotransmitter release offering a path for serotonin's effects. As part of a pentameric complex 5-HT3A subunits combine to form functional ion channels. The receptor particularly influences excitatory neurotransmission affecting processes involved in mood regulation nausea and vomiting. Consequently this receptor serves key physiological functions through its modulation of ionic flux and neuronal activity.
- Pathways
The 5-HT3A receptor plays an essential role in pathways related to neurotransmission and neuroplasticity. Within serotonin signalling pathways 5HT3A closely associates with proteins such as serotonin transporter (SERT) elaborating on its role in serotonin uptake and homeostasis. Furthermore its participation in these pathways links to the regulation of calcium-dependent processes interacting with calmodulins and other calcium-binding proteins to influence downstream signalling events essential for cognitive processes and sensory signalling.
- Associated diseases and disorders
The 5-HT3A receptor has been associated with conditions like anxiety and irritable bowel syndrome (IBS). In anxiety disorders its role in modulating serotonin-induced neurotransmission points to its involvement in mood disorders where altered serotonin balance is a contributing factor. As for IBS the receptor's high expression in the gut highlights its influence on gastrointestinal motility and secretion. Researchers also explore its connection with GABAergic systems pointing to potential therapeutic targets for managing symptoms in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)
All lanes: Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897) at 3 µg/mL
Lane 1: Human Brain tissue lysate
Lane 2: Mouse Brain tissue lysate
Lane 3: Rat Brain tissue lysate
Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (ab13897)
IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)
Western Blot of human brain lysate staining 5HT3A receptor with ab13897
All lanes: Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)
Performed under reducing conditions.
Predicted band size: 55 kDa
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