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Rabbit Recombinant Monoclonal 5HT3A receptor/5ht3 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (AB271031), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (AB271031), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (AB271031), expandable thumbnail
  • Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (AB271031), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (AB271031), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Not recommended
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Rat
Dilution info
1/5000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.

Alternative names

5ht3, Htr3, Htr3a, 5-hydroxytryptamine receptor 3A, 5-HT3-A, 5-HT3A, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor, 5-HT-3, 5-HT3R

Recommended products

Rabbit Recombinant Monoclonal 5HT3A receptor/5ht3 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24323-75
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The 5HT3A receptor also known as the 5-HT3 receptor or 5HT3 forms part of the serotonin receptor family. These ligand-gated ion channels enable the rapid conduction of cations such as Na+ and Ca2+ into cells upon activation. The 5-HT3A receptor exhibits a molecular weight of approximately 55 to 60 kDa and is expressed broadly in peripheral and central nervous systems including regions like the cortex hippocampus and gastrointestinal tract. This expression pattern allows it to perform roles in neurotransmission and modulate physiological responses across different systems.

Biological function summary

The 5-HT3A receptor acts as a critical mediator of synaptic signalling and neurotransmitter release offering a path for serotonin's effects. As part of a pentameric complex 5-HT3A subunits combine to form functional ion channels. The receptor particularly influences excitatory neurotransmission affecting processes involved in mood regulation nausea and vomiting. Consequently this receptor serves key physiological functions through its modulation of ionic flux and neuronal activity.

Pathways

The 5-HT3A receptor plays an essential role in pathways related to neurotransmission and neuroplasticity. Within serotonin signalling pathways 5HT3A closely associates with proteins such as serotonin transporter (SERT) elaborating on its role in serotonin uptake and homeostasis. Furthermore its participation in these pathways links to the regulation of calcium-dependent processes interacting with calmodulins and other calcium-binding proteins to influence downstream signalling events essential for cognitive processes and sensory signalling.

Associated diseases and disorders

The 5-HT3A receptor has been associated with conditions like anxiety and irritable bowel syndrome (IBS). In anxiety disorders its role in modulating serotonin-induced neurotransmission points to its involvement in mood disorders where altered serotonin balance is a contributing factor. As for IBS the receptor's high expression in the gut highlights its influence on gastrointestinal motility and secretion. Researchers also explore its connection with GABAergic systems pointing to potential therapeutic targets for managing symptoms in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031), expandable thumbnail

    Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed in mouse spleen/brain is consistent with the literature (PMID: 12388212).

    All lanes: Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031) at 1/5000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 10 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa

    Observed band size: 55 kDa

    Exposure time: 6s

  • Immunocytochemistry/ Immunofluorescence - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling 5HT3A receptor/5ht3 with ab271031 at 1/50 (11.44 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed.

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Flow Cytometry (Intracellular) - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling 5HT3A receptor/5ht3 with ab271031 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody.

  • Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031), expandable thumbnail

    Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031) at 1/5000 dilution

    Lane 1: Mouse hippocampus tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Lane 3: Rat spinal cord at 20 µg

    Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa

    Observed band size: 55 kDa

    Exposure time: 8s

  • Immunocytochemistry/ Immunofluorescence - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling 5HT3A receptor/5ht3 with ab271031 at 1/50 (11.44 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Confocal image showing positive staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed.

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Flow Cytometry (Intracellular) - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-5HT3A receptor/5ht3 antibody [EPR24323-75] (ab271031)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells cells labelling 5HT3A receptor/5ht3 with ab271031 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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