AB60359

Anti-5HT4 Receptor antibody

Name: Anti-5HT4 Receptor antibody (ab60359) | Abcam
Brand: Abcam Limited
SKU: AB60359
Price: 450 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(15 Publications)

Rabbit Polyclonal 5HT4 Receptor antibody. Suitable for WB, ICC/IF and reacts with Zebrafish, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human HTR4 aa 1-100.

View Alternative Names

5-hydroxytryptamine receptor 4, 5-HT-4, 5-HT4, Serotonin receptor 4, HTR4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (AB60359)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (AB60359)

ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right).

Unknown

Western blot - Anti-5HT4 Receptor antibody (AB60359)

All lanes:

Western blot - Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution

Lane 1:

Marker

Lane 2:

Zebrafish brain homogenate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 44 kDa

Observed band size: 42 kDa

true

Exposure time: 5min

Supplier Data

Western blot - Anti-5HT4 Receptor antibody (AB60359)

All lanes:

Western blot - Anti-5HT4 Receptor antibody (ab60359)

Lane 1:

Jurkat cell lysate

Lane 2:

COLO205 cell lysate

Lane 3:

COLO205 cell lysate with Synthesized peptide

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Zebrafish

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human HTR4 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q13639

Specificity

5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5-HT4 receptor also known as 5-hydroxytryptamine receptor 4 is a G protein-coupled receptor (GPCR) that largely facilitates neurotransmission. Mechanically 5-HT4 receptors bind serotonin leading to the activation of adenylate cyclase which increases cellular levels of cyclic AMP. The 5-HT4 receptor has a molecular mass of approximately 40 kDa. It is widely expressed in the central nervous system such as in the brain as well as in peripheral tissues including the gastrointestinal tract and heart.

Biological function summary

The 5-HT4 receptor plays key roles in modulating brain processes related to memory and cognition and regulates gut motility. While the receptor acts independently it does not form part of larger receptor complexes. Its ability to influence neurotransmitter release makes it important for synaptic plasticity and smooth muscle contraction in the gastrointestinal tract.

Pathways

The 5-HT4 receptor is integral in the serotonergic signaling pathway which governs mood cognition and gastrointestinal function. Additionally the receptor interacts with downstream elements of the cyclic AMP signaling pathway linking to proteins such as protein kinase A that subsequently affect gene expression and modulation of ion channels.

The 5-HT4 receptor has connections with neurodegenerative diseases like Alzheimer’s disease and gastrointestinal disorders such as irritable bowel syndrome. Dysregulation of the receptor's signaling may lead to these conditions through interactions with proteins like tau in Alzheimer's disease or other neurotransmitter systems impacting gut motility disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed : 10821780, PubMed : 16102731, PubMed : 35714614, PubMed : 9603189). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 16102731, PubMed : 35714614). HTR4 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed : 16102731, PubMed : 35714614).

See full target information HTR4

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14187 PubMed38716507

2024

Amygdala neuronal dyshomeostasis via 5-HT receptors mediates mood and cognitive defects in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Rong Wu,Xiao-Na Zhu,Yuan-Bo Pan,Xue Gu,Xian-Dong Liu,Si Chen,Yu Zhang,Tian-Le Xu,Nan-Jie Xu,Suya Sun

Histology and histopathology 39:903-919 PubMed38108436

2023

Differential roles of serotonin receptor subtypes in regulation of neurotrophin receptor expression and intestinal hypernociception.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ping She,Yu-Ting Hsieh,Li-Yu Lin,Chia-Hung Tu,Ming-Shiang Wu,Ling-Wei Hsin,Linda Chia-Hui Yu

Evidence-based complementary and alternative medicine : eCAM 2022:8659462 PubMed36337584

2022

Electroacupuncture Modulates 5-HT-Mediated cAMP/PKA Signaling to Improve Intestinal Motility Disorders in a Thy1-Syn Parkinson's Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Shen,Zhaoqin Wang,Rude Huang,Luyi Wu,Yan Huang,Qin Qi,Rui Zhong,Yiyi Chen,Lingjie Li,Huangan Wu

European archives of psychiatry and clinical neuroscience 273:411-425 PubMed36094569

2022

Elevated levels of serotonin 5-HT receptors in the orbitofrontal cortex of antisocial individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Braccagni,Simona Scheggi,Marco Bortolato

Frontiers in physiology 13:990178 PubMed36176773

2022

Activation of uroepithelial 5-HTR inhibits mechanosensory activity of murine bladder afferent nerves.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Lu,Jie Li,Li Dong,Ping Luo,Guohua Zhang,Weifang Rong

Virologica Sinica 38:9-22 PubMed36007838

2022

Domesticated HERV-W env contributes to the activation of the small conductance Ca-activated K type 2 channels via decreased 5-HT4 receptor in recent-onset schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiulin Wu,Qiujin Yan,Lianzhong Liu,Xing Xue,Wei Yao,Xuhang Li,Wenshi Li,Shuang Ding,Yaru Xia,Dongyan Zhang,Fan Zhu

Laboratory investigation; a journal of technical methods and pathology 102:1023-1037 PubMed35585132

2022

5-HT receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Ying Chang,Yi-Ting Yang,Meng-Ping She,Chia-Hung Tu,Tsung-Chun Lee,Ming-Shiang Wu,Chin-Hung Sun,Ling-Wei Hsin,Linda Chia-Hui Yu

Frontiers in pharmacology 13:804723 PubMed35496291

2022

Effects of Maren Pills on the Intestinal Microflora and Short-Chain Fatty Acid Profile in Drug-Induced Slow Transit Constipation Model Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhan,Yong Wen,Li-Juan Du,Xiao-Xiang Wang,Shi-Yu Tang,Peng-Fei Kong,Wei-Guo Huang,Xue-Gui Tang

Molecular biology and evolution 39: PubMed35277964

2022

Quantitative Lipidomics and Spatial MS-Imaging Uncovered Neurological and Systemic Lipid Metabolic Pathways Underlying Troglomorphic Adaptations in Cave-Dwelling Fish.

Applications

Unspecified application

Species

Unspecified reactive species

Sin Man Lam,Jie Li,Huan Sun,Weining Mao,Zongmin Lu,Qingshuo Zhao,Chao Han,Xia Gong,Binhua Jiang,Gek Huey Chua,Zhenwen Zhao,Fanwei Meng,Guanghou Shui

Pharmaceutics 13: PubMed34834271

2021

Blood-Brain Barrier Permeability: Is 5-Hydroxytryptamine Receptor Type 4 a Game Changer?

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Becker,Sylvia Da Silva,Amelia-Naomi Sabo,Maria Cristina Antal,Véronique Kemmel,Laurent Monassier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-5HT4 Receptor antibody

Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (AB60359)

Western blot - Anti-5HT4 Receptor antibody (AB60359)

Western blot - Anti-5HT4 Receptor antibody (AB60359)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise