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Rabbit Recombinant Monoclonal 5HT4 Receptor antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (AB317501), expandable thumbnail
  • Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (AB317501), expandable thumbnail
  • Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (AB317501), expandable thumbnail
  • Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (AB317501), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrFlow Cyt (Intra)IHC-PICC/IFIP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed:10821780, PubMed:16102731, PubMed:35714614, PubMed:9603189). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:16102731, PubMed:35714614). HTR4 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed:16102731, PubMed:35714614).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal 5HT4 Receptor antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28675-19

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab317501 is the carrirer-free version of Anti-5HT4 Receptor antibody [EPR28675-19] ab317500.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 5-HT4 receptor also known as 5-hydroxytryptamine receptor 4 is a G protein-coupled receptor (GPCR) that largely facilitates neurotransmission. Mechanically 5-HT4 receptors bind serotonin leading to the activation of adenylate cyclase which increases cellular levels of cyclic AMP. The 5-HT4 receptor has a molecular mass of approximately 40 kDa. It is widely expressed in the central nervous system such as in the brain as well as in peripheral tissues including the gastrointestinal tract and heart.

Biological function summary

The 5-HT4 receptor plays key roles in modulating brain processes related to memory and cognition and regulates gut motility. While the receptor acts independently it does not form part of larger receptor complexes. Its ability to influence neurotransmitter release makes it important for synaptic plasticity and smooth muscle contraction in the gastrointestinal tract.

Pathways

The 5-HT4 receptor is integral in the serotonergic signaling pathway which governs mood cognition and gastrointestinal function. Additionally the receptor interacts with downstream elements of the cyclic AMP signaling pathway linking to proteins such as protein kinase A that subsequently affect gene expression and modulation of ion channels.

Associated diseases and disorders

The 5-HT4 receptor has connections with neurodegenerative diseases like Alzheimer’s disease and gastrointestinal disorders such as irritable bowel syndrome. Dysregulation of the receptor's signaling may lead to these conditions through interactions with proteins like tau in Alzheimer's disease or other neurotransmitter systems impacting gut motility disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501), expandable thumbnail

    Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501)

    This data was developed using Anti-5HT4 Receptor antibody [EPR28675-19] ab317500, the same antibody clone in a different buffer formulation.

    Low expression tissue: Skeletal muscle, liver (PMID: 15118808).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (Anti-5HT4 Receptor antibody [EPR28675-19] ab317500) at 1/1000 dilution

    Lane 1: Mouse placenta tissue lysate at 20 µg

    Lane 2: Mouse striatum tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Rat placenta tissue lysate at 20 µg

    Lane 5: Rat striatum tissue lysate at 20 µg

    Lane 6: Rat skeletal muscle tissue lysate at 20 µg

    Lane 7: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 43 kDa, 36 kDa

    Exposure time: 180s

    Low expression tissue: Skeletal muscle, liver (PMID: 15118808).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501), expandable thumbnail

    Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501)

    This data was developed using Anti-5HT4 Receptor antibody [EPR28675-19] ab317500, the same antibody clone in a different buffer formulation.

    The identity of the lower MW band at approximately 28 kDa (in lane 3) is unknown.

    All lanes: Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (Anti-5HT4 Receptor antibody [EPR28675-19] ab317500) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 43 kDa

    Exposure time: 180s

    The identity of the lower MW band at approximately 28 kDa (in lane 3) is unknown.

  • Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501), expandable thumbnail

    Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501)

    This data was developed using Anti-5HT4 Receptor antibody [EPR28675-19] ab317500, the same antibody clone in a different buffer formulation.

    Low expression tissue: skeletal muscle (PMID: 15118808).

    The identity of the bands higher than 50 kDa and lower than 37 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-4: 59 seconds; Lanes 5-6: 180 seconds.

    All lanes: Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (Anti-5HT4 Receptor antibody [EPR28675-19] ab317500) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human placenta tissue lysate at 20 µg

    Lane 3: Human striatum tissue lysate at 20 µg

    Lane 4: Human skeletal muscle tissue lysate at 20 µg

    Lane 5: Human tonsil tissue lysate at 20 µg

    Lane 6: IMR-90 (human lung fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 43 kDa, 36 kDa

  • Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501), expandable thumbnail

    Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] - BSA and Azide free (ab317501)

    This data was developed using Anti-5HT4 Receptor antibody [EPR28675-19] ab317500, the same antibody clone in a different buffer formulation.

    Low expression tissue: liver (PMID: 15118808).

    The identity of the band higher than 50 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (Anti-5HT4 Receptor antibody [EPR28675-19] ab317500) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 43 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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