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Rabbit Recombinant Monoclonal 5HT7 Receptor antibody. Suitable for WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-5HT7 Receptor antibody [EPR28676-1] (AB317565), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (AB317565), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (AB317565), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (AB317565), expandable thumbnail
  • Western blot - Anti-5HT7 Receptor antibody [EPR28676-1] (AB317565), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)IHC-PIHC-FrICC/IF
Human
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/30
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Target data

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed:8394362, PubMed:8397408, PubMed:8398139). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:8394362). HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed:8394362).

Alternative names

5-hydroxytryptamine receptor 7, 5-HT-7, 5-HT7, 5-HT-X, GPRFO, Serotonin receptor 7

Recommended products

Rabbit Recombinant Monoclonal 5HT7 Receptor antibody. Suitable for WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28676-1
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human IHC-P and rat IHC-P.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The 5-HT7 receptor also known as the serotonin receptor 5-HT7 is a G-protein-coupled receptor that plays a mechanical role in neurotransmission. This receptor belongs to the serotonin receptor family and typically weighs around 46 kDa. You find the 5-HT7 receptor expressed in various tissues including the brain particularly in regions such as the thalamus hypothalamus and hippocampus as well as in smooth muscles and the gastrointestinal tract. Its function is mediated through Gs proteins which activate adenylate cyclase leading to increased levels of cyclic adenosine monophosphate (cAMP).

Biological function summary

The 5-HT7 receptor contributes to multiple physiological processes including the regulation of circadian rhythms mood and cognitive functions. It operates as part of complex signaling networks within the central nervous system and other systems throughout the body. The receptor plays a significant role in modulating neurotransmitter release and neuronal excitability. Through these mechanisms it influences various behavioral and physiological outputs that are critical for the maintenance of homeostasis.

Pathways

The 5-HT7 receptor is a pivotal component of serotoninergic signaling especially influencing the cAMP signaling cascade. This pathway interacts with proteins like adenylate cyclase and cAMP-dependent protein kinase impacting functions such as learning and memory. The receptor's modulation of cAMP levels places it within important pathways that affect cell signaling and metabolism including the broader serotonergic system which involves other serotonin receptors such as 5-HT1A and 5-HT2A.

Associated diseases and disorders

Alterations in the function or expression of the 5-HT7 receptor are linked to a variety of conditions. It has been implicated in psychiatric disorders such as depression and schizophrenia where disruptions in serotonin signaling are common. In these contexts the receptor's interaction with other neurotransmitter systems including dopamine comes into play with proteins like the dopamine D2 receptor being relevant. The 5-HT7 receptor is also associated with migraine as it plays a role in the dilation of cerebral blood vessels and transmission of nociceptive signals making it a target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565), expandable thumbnail

    Immunoprecipitation - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565)

    5HT7 Receptor was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab317565 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317565 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate

    Lane 2: ab317565 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317565 in rat brain tissue lysate

    Samples are non-boiled as boiling may cause protein aggregation.

    All lanes: Immunoprecipitation - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565) at 1/30 dilution

    All lanes: Rat brain tissue lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling 5HT7 Receptor with ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

    Negative control: No staining in mouse liver.

    The section was incubated with ab317565 at 4°C overnight.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling 5HT7 Receptor with ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

    Positive staining in mouse hippocampus.

    The section was incubated with ab317565 at 4°C overnight.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling 5HT7 Receptor with ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

    Positive staining in mouse cerebrum (PMID: 28690143).

    The section was incubated with ab317565 at 4°C overnight.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565), expandable thumbnail

    Western blot - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565)

    Low expression: skeletal muscle (PMID: 8394362), liver (PMID: 8394362), L6.

    The identity of the lower MW band at approximately 15 kDa (in lane 8 and lane 10) is unknown.

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-5HT7 Receptor antibody [EPR28676-1] (ab317565) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Rat hypothalamus tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Rat skeletal muscle tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Rat liver tissue lysate at 20 µg with NFDM/TBST

    Lane 5: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 6: Mouse brain tissue lysate at 20 µg with NFDM/TBST

    Lane 7: Mouse liver tissue lysate at 20 µg with NFDM/TBST

    Lane 8: Human cerebellum tissue lysate at 20 µg with NFDM/TBST

    Lane 9: Human liver tissue lysate at 20 µg with NFDM/TBST

    Lane 10: Human brain tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 59s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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