Anti-5HT7 Receptor antibody [EPR28676-1] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] - BSA and Azide free (AB317566)

This data was developed using ab317565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling 5HT7 Receptor with ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Negative control : No staining in mouse liver.
The section was incubated with ab317565 at 4°C overnight.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] - BSA and Azide free (AB317566)

This data was developed using ab317565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling 5HT7 Receptor with ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining in mouse cerebrum (PMID : 28690143).
The section was incubated with ab317565 at 4°C overnight.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody [EPR28676-1] - BSA and Azide free (AB317566)

This data was developed using ab317565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling 5HT7 Receptor with ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining in mouse hippocampus.
The section was incubated with ab317565 at 4°C overnight.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-5HT7 Receptor antibody [EPR28676-1] - BSA and Azide free (AB317566)

This data was developed using ab317565, the same antibody clone in a different buffer formulation.

5HT7 Receptor was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab317565 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317565 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate
Lane 2 : ab317565 IP in Rat brain tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317565 in rat brain tissue lysate

Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Immunoprecipitation - Anti-5HT7 Receptor antibody [EPR28676-1] (<a href='/en-us/products/primary-antibodies/5ht7-receptor-antibody-epr28676-1-ab317565'>ab317565</a>) at 1/30 dilution

All lanes:

Rat brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-5HT7 Receptor antibody [EPR28676-1] - BSA and Azide free (AB317566)

This data was developed using ab317565, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle (PMID : 8394362), liver (PMID : 8394362), L6.

The identity of the lower MW band at approximately 15 kDa (in lane 8 and lane 10) is unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-5HT7 Receptor antibody [EPR28676-1] (<a href='/en-us/products/primary-antibodies/5ht7-receptor-antibody-epr28676-1-ab317565'>ab317565</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat hypothalamus tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Lane 5:

L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Mouse liver tissue lysate at 20 µg

Lane 8:

Human cerebellum tissue lysate at 20 µg

Lane 9:

Human liver tissue lysate at 20 µg

Lane 10:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 59s