AB128892

Anti-5HT7 Receptor antibody [EPR6271]

RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal 5HT7 Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

5-hydroxytryptamine receptor 7, 5-HT-7, 5-HT7, 5-HT-X, Serotonin receptor 7, HTR7

2 Images

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Western blot - Anti-5HT7 Receptor antibody [EPR6271] (AB128892)

All lanes:

Western blot - Anti-5HT7 Receptor antibody [EPR6271] (ab128892) at 1/1000 dilution

Lane 1:

U-87 MG cell lysate at 10 µg

Lane 2:

SK-OV-3 cell lysate at 10 µg

Lane 3:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa

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OI-RD Scanning - Anti-5HT7 Receptor antibody [EPR6271] (AB128892)

OI-RD Scanning

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OI-RD Scanning - Anti-5HT7 Receptor antibody [EPR6271] (AB128892)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6271

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5-HT7 receptor also known as the serotonin receptor 5-HT7 is a G-protein-coupled receptor that plays a mechanical role in neurotransmission. This receptor belongs to the serotonin receptor family and typically weighs around 46 kDa. You find the 5-HT7 receptor expressed in various tissues including the brain particularly in regions such as the thalamus hypothalamus and hippocampus as well as in smooth muscles and the gastrointestinal tract. Its function is mediated through Gs proteins which activate adenylate cyclase leading to increased levels of cyclic adenosine monophosphate (cAMP).

Biological function summary

The 5-HT7 receptor contributes to multiple physiological processes including the regulation of circadian rhythms mood and cognitive functions. It operates as part of complex signaling networks within the central nervous system and other systems throughout the body. The receptor plays a significant role in modulating neurotransmitter release and neuronal excitability. Through these mechanisms it influences various behavioral and physiological outputs that are critical for the maintenance of homeostasis.

Pathways

The 5-HT7 receptor is a pivotal component of serotoninergic signaling especially influencing the cAMP signaling cascade. This pathway interacts with proteins like adenylate cyclase and cAMP-dependent protein kinase impacting functions such as learning and memory. The receptor's modulation of cAMP levels places it within important pathways that affect cell signaling and metabolism including the broader serotonergic system which involves other serotonin receptors such as 5-HT1A and 5-HT2A.

Alterations in the function or expression of the 5-HT7 receptor are linked to a variety of conditions. It has been implicated in psychiatric disorders such as depression and schizophrenia where disruptions in serotonin signaling are common. In these contexts the receptor's interaction with other neurotransmitter systems including dopamine comes into play with proteins like the dopamine D2 receptor being relevant. The 5-HT7 receptor is also associated with migraine as it plays a role in the dilation of cerebral blood vessels and transmission of nociceptive signals making it a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed : 35714614, PubMed : 8226867). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 35714614, PubMed : 8226867). HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed : 35714614).

See full target information HTR7

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 137: PubMed39279505

2024

The interplay of serotonin 5-HT1A and 5-HT7 receptors in chronic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Bijata,Alexander Wirth,Jakub Wlodarczyk,Evgeni Ponimaskin

International journal of molecular sciences 25: PubMed38892090

2024

Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Viktor Stratilov,Sofiya Potapova,Diana Safarova,Ekaterina Tyulkova,Oleg Vetrovoy

The EMBO journal 43:2636-2660 PubMed38778156

2024

The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate.

Applications

Unspecified application

Species

Unspecified reactive species

Pushpak Bhattacharjee,Die Wang,Dovile Anderson,Joshua N Buckler,Eveline de Geus,Feng Yan,Galina Polekhina,Ralf Schittenhelm,Darren J Creek,Lawrence D Harris,Anthony J Sadler

Frontiers in cellular neuroscience 16:922750 PubMed36072567

2022

Tandospirone prevents stress-induced anxiety-like behavior and visceral hypersensitivity by suppressing theta oscillation enhancement 5-HT receptors in the anterior cingulate cortex in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Ting Zhan,Zhi-Yu Dong,Li-Sha Yi,Yan Zhang,Hui-Hui Sun,Hai-Qin Zhang,Jun-Wen Wang,Ying Chen,Ying Huang,Shu-Chang Xu

Journal of neuroscience research 100:1506-1523 PubMed35443076

2022

Serotonin 5-HT receptor overexpression in the raphe nuclei area produces antidepressive effect and affects brain serotonin system in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Ya Rodnyy,Elena M Kondaurova,Darya V Bazovkina,Elisabeth A Kulikova,Tatiana V Ilchibaeva,Alexandra I Kovetskaya,Irina A Baraboshkina,Ekaterina Yu Bazhenova,Nina K Popova,Vladimir S Naumenko

Frontiers in neuroscience 15:668616 PubMed34163324

2021

Determining 5HTR's Involvement in Modifying the Antihyperalgesic Effects of Electroacupuncture on Rats With Recurrent Migraine.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Liu,Xiao-Bai Xu,Zheng-Yang Qu,Luo-Peng Zhao,Claire-Shuiqing Zhang,Zhi-Juan Li,Tian-Li Lyu,Xue-Fei Wang,Xiang-Hong Jing,Bin Li

International journal of molecular sciences 21: PubMed33233644

2020

Genetic Background Underlying 5-HT Receptor Functioning Affects the Response to Fluoxetine.

Applications

Unspecified application

Species

Unspecified reactive species

Elena M Kondaurova,Alexander Ya Rodnyy,Tatiana V Ilchibaeva,Anton S Tsybko,Dmitry V Eremin,Yegor V Antonov,Nina K Popova,Vladimir S Naumenko

Physiological genomics 51:290-301 PubMed31125290

2019

Creation of the 5-hydroxytryptamine receptor 7 knockout rat as a tool for cardiovascular research.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Y Demireva,Huirong Xie,Emma D Flood,Janice M Thompson,Bridget M Seitz,Stephanie W Watts

BJU international 123:718-725 PubMed29781566

2018

The serotonin (5-hydroxytryptamine) 5-HT receptor is up-regulated in Onuf's nucleus in rats with chronic spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jianshu Ni,Nailong Cao,Xiaohu Wang,Changsheng Zhan,Jiemin Si,Baojun Gu,Karl-Erik Andersson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-5HT7 Receptor antibody [EPR6271]

Western blot - Anti-5HT7 Receptor antibody [EPR6271] (AB128892)

OI-RD Scanning - Anti-5HT7 Receptor antibody [EPR6271] (AB128892)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise