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AB248203

Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free

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Rabbit Recombinant Monoclonal 5HT7 Receptor antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

5-hydroxytryptamine receptor 7, 5-HT-7, 5-HT7, 5-HT-X, Serotonin receptor 7, HTR7

2 Images
Western blot - Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free (AB248203)
  • WB

Unknown

Western blot - Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free (AB248203)

This data was developed using ab128892, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-5HT7 Receptor antibody [EPR6271] (<a href='/en-us/products/primary-antibodies/5ht7-receptor-antibody-epr6271-ab128892'>ab128892</a>) at 1/1000 dilution

Lane 1:

U-87 MG cell lysate at 10 µg

Lane 2:

SK-OV-3 cell lysate at 10 µg

Lane 3:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa

false

OI-RD Scanning - Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free (AB248203)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free (AB248203)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6271

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248203 is the carrier-free version of ab128892.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5-HT7 receptor also known as the serotonin receptor 5-HT7 is a G-protein-coupled receptor that plays a mechanical role in neurotransmission. This receptor belongs to the serotonin receptor family and typically weighs around 46 kDa. You find the 5-HT7 receptor expressed in various tissues including the brain particularly in regions such as the thalamus hypothalamus and hippocampus as well as in smooth muscles and the gastrointestinal tract. Its function is mediated through Gs proteins which activate adenylate cyclase leading to increased levels of cyclic adenosine monophosphate (cAMP).
Biological function summary

The 5-HT7 receptor contributes to multiple physiological processes including the regulation of circadian rhythms mood and cognitive functions. It operates as part of complex signaling networks within the central nervous system and other systems throughout the body. The receptor plays a significant role in modulating neurotransmitter release and neuronal excitability. Through these mechanisms it influences various behavioral and physiological outputs that are critical for the maintenance of homeostasis.

Pathways

The 5-HT7 receptor is a pivotal component of serotoninergic signaling especially influencing the cAMP signaling cascade. This pathway interacts with proteins like adenylate cyclase and cAMP-dependent protein kinase impacting functions such as learning and memory. The receptor's modulation of cAMP levels places it within important pathways that affect cell signaling and metabolism including the broader serotonergic system which involves other serotonin receptors such as 5-HT1A and 5-HT2A.

Alterations in the function or expression of the 5-HT7 receptor are linked to a variety of conditions. It has been implicated in psychiatric disorders such as depression and schizophrenia where disruptions in serotonin signaling are common. In these contexts the receptor's interaction with other neurotransmitter systems including dopamine comes into play with proteins like the dopamine D2 receptor being relevant. The 5-HT7 receptor is also associated with migraine as it plays a role in the dilation of cerebral blood vessels and transmission of nociceptive signals making it a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed : 35714614, PubMed : 8226867). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 35714614, PubMed : 8226867). HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed : 35714614).
See full target information HTR7
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Product promise

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For full details, please see our Terms & Conditions

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