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AB134162

Anti-5T4 antibody [EPR5529]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

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(8 Publications)

Anti-5T4 antibody [EPR5529] (ab134162) is a rabbit monoclonal antibody detecting 5T4 in Western Blot, IHC-P. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

5T4, TPBG, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, M6P1, Wnt-activated inhibitory factor 1, 5T4 oncotrophoblast glycoprotein, WAIF1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)

Immunohistochemical staining using ab134162 on paraffin embedded human placenta tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling 5T4 with unpurified ab134162 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)

Immunohistochemical staining using ab134162 on paraffin embedded human endometrial cancer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (AB134162)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling 5T4 with purified ab134162 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Western blot - Anti-5T4 antibody [EPR5529] (AB134162)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5529] (AB134162)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-5T4 antibody [EPR5529] (ab134162) at 1/5000 dilution

All lanes:

HT-29 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 72-80 kDa

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Western blot - Anti-5T4 antibody [EPR5529] (AB134162)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5529] (AB134162)

Lanes 1 - 4 : Merged signal (red and green). Green - ab134162 observed at 85 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab134162 was shown to react with 5T4 in wild-type MCF7 cells in Western blot with loss of signal observed in TPBG knockout cell line ab269499 (knockout cell lysate ab269661). Wild-type MCF7 and TPBG knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in before incubation with ab134162 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-5T4 antibody [EPR5529] (ab134162) at 1/1000 dilution

Lane 1:

Wild-type MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TPBG knockout MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TPBG knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-tpbg-knockout-mcf7-cell-line-ab269499'>ab269499</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 85 kDa

false

Western blot - Anti-5T4 antibody [EPR5529] (AB134162)
  • WB

Unknown

Western blot - Anti-5T4 antibody [EPR5529] (AB134162)

All lanes:

Western blot - Anti-5T4 antibody [EPR5529] (ab134162) at 1/1000 dilution

Lane 1:

HT-29 cell lysates at 10 µg

Lane 2:

HT-1376 cell lysates at 10 µg

Lane 3:

Caco-2 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-5T4 antibody [EPR5529] (AB134162)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5529] (AB134162)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-5T4 antibody [EPR5529] (ab134162) at 1/500 dilution

All lanes:

HT-29 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 72-80 kDa

false

OI-RD Scanning - Anti-5T4 antibody [EPR5529] (AB134162)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-5T4 antibody [EPR5529] (AB134162)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-5T4 antibody [EPR5529] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5529

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/3", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100.</strong></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-5T4 antibody [EPR5529] (ab134162) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of 5T4?
Anti-5T4 [EPR5529] (ab134162) specifically detects a band for 5T4 (UniProt: Q13641) at a molecular weight of 46kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-5T4 antibody [EPR5529] (ab134162) has been confirmed by Western blot testing in TPBG Knockout MCF7 cell line, ab269499.

Other related products
We have a range of other formats of antibody clone [EPR5529] also available for your convenience: ab134162, Carrier free - ab201711

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5T4 protein also known as trophoblast glycoprotein or B3F1 is a cell surface antigen with a mass of approximately 72 kDa. It is encoded by the TPBG gene and expresses mainly in embryonic tissues and various cancers with limited expression in normal adult tissues. The 5T4 protein is characterized by a densely glycosylated structure contributing to its role in cellular processes like adhesion and migration.
Biological function summary

5T4 is involved in processes associated with embryonic development and cellular mobility. It contributes to the epithelial-to-mesenchymal transition (EMT) which is a critical process in both normal development and cancer metastasis. Although 5T4 is not part of a known specific complex its glycoprotein nature allows interaction with the extracellular matrix and other cell surface proteins influencing cell behavior and morphology.

Pathways

5T4 plays a part in signaling pathways involved in cell migration and invasion such as the Wnt/Β-catenin pathway and TGF-Β signaling. In the context of the Wnt/Β-catenin pathway 5T4 interacts with proteins like Β-catenin influencing cell proliferation and survival. Through its involvement in TGF-Β signaling 5T4 can impact cell differentiation processes and regulatory mechanisms often linking to other proteins such as SMAD family members within these pathways.

5T4 is associated with cancer particularly in its role in promoting metastasis. Its expression is notably increased in several carcinomas including breast and colorectal cancers. The elevated levels of 5T4 in tumors correlate with poorer prognosis making it a potential target for cancer therapies. Additionally 5T4 has connections to proteins such as MMPs (matrix metalloproteinases) which facilitate tissue remodeling and metastatic spread highlighting its significance in cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
See full target information TPBG
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed34638324

2021

High Prevalence of 5T4/Trophoblast Glycoprotein in Soft Tissue Sarcomas.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Groothuis,Nicola Penel,Antoine Italiano,Nuria Kotecki,Fred Dijcks,Wim Dokter

Targeted oncology 14:465-477 PubMed31332693

2019

Combination Treatment with an Antibody-Drug Conjugate (A1mcMMAF) Targeting the Oncofetal Glycoprotein 5T4 and Carboplatin Improves Survival in a Xenograft Model of Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Y Louise Wan,Puja Sapra,James Bolton,Jia Xin Chua,Lindy G Durrant,Peter L Stern

PloS one 12:e0187449 PubMed29216196

2017

Loss of epithelial markers is an early event in oral dysplasia and is observed within the safety margin of dysplastic and T1 OSCC biopsies.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Abdalla,Tanya Walsh,Nalin Thakker,Christopher M Ward

PloS one 12:e0179561 PubMed28727782

2017

Detecting expression of 5T4 in CTCs and tumor samples from NSCLC patients.

Applications

Unspecified application

Species

Unspecified reactive species

Steven R Pirie-Shepherd,Cory Painter,Pamela Whalen,Pamela Vizcarra,Marc Roy,Jesse Qian,Tania Franks,Tim Coskran,Jon Golas,Shibing Deng,Wenyan Zhong,Eric Tucker,Dena Marrinucci,Hans-Peter Gerber,Eric L Powell

Scientific reports 7:4859 PubMed28687750

2017

5T4-specific chimeric antigen receptor modification promotes the immune efficacy of cytokine-induced killer cells against nasopharyngeal carcinoma stem cell-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyang Guo,Hang Zheng,Weiren Luo,Qianbing Zhang,Jingxian Liu,Kaitai Yao

Clinical cancer research : an official journal of the American Association for Cancer Research 23:2516-2527 PubMed27780858

2016

5T4-Targeted Therapy Ablates Cancer Stem Cells and Prevents Recurrence of Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel A Kerk,Kelsey A Finkel,Alexander T Pearson,Kristy A Warner,Zhaocheng Zhang,Felipe Nör,Vivian P Wagner,Pablo A Vargas,Max S Wicha,Elaine M Hurt,Robert E Hollingsworth,David A Tice,Jacques E Nör

Cell 166:1295-1307.e21 PubMed27565350

2016

Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.

Applications

Unspecified application

Species

Unspecified reactive species

Ken H Loh,Philipp S Stawski,Austin S Draycott,Namrata D Udeshi,Emily K Lehrman,Daniel K Wilton,Tanya Svinkina,Thomas J Deerinck,Mark H Ellisman,Beth Stevens,Steven A Carr,Alice Y Ting

Molecular cancer therapeutics 12:38-47 PubMed23223830

2012

Long-term tumor regression induced by an antibody-drug conjugate that targets 5T4, an oncofetal antigen expressed on tumor-initiating cells.

Applications

IHC-P

Species

Unspecified reactive species

Puja Sapra,Marc Damelin,John Dijoseph,Kimberly Marquette,Kenneth G Geles,Jonathon Golas,Maureen Dougher,Bitha Narayanan,Andreas Giannakou,Kiran Khandke,Russell Dushin,Elana Ernstoff,Judy Lucas,Mauricio Leal,George Hu,Christopher J O'Donnell,Lioudmila Tchistiakova,Robert T Abraham,Hans-Peter Gerber
View all publications
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