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AB129058

Anti-5T4 antibody [EPR5530]

4

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal 5T4 antibody. Suitable for IP, WB and reacts with Human, Rat samples. Cited in 7 publications.

View Alternative Names

5T4, TPBG, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, M6P1, Wnt-activated inhibitory factor 1, 5T4 oncotrophoblast glycoprotein, WAIF1

8 Images
Immunoprecipitation - Anti-5T4 antibody [EPR5530] (AB129058)
  • IP

Lab

Immunoprecipitation - Anti-5T4 antibody [EPR5530] (AB129058)

ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)

Predicted band size: 46 kDa

false

Immunoprecipitation - Anti-5T4 antibody [EPR5530] (AB129058)
  • IP

Lab

Immunoprecipitation - Anti-5T4 antibody [EPR5530] (AB129058)

ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)

Predicted band size: 46 kDa

false

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution

Lane 1:

HT-1376 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)

Lanes 1 - 4 : Merged signal (red and green). Green - ab129058 observed at 85 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab129058 was shown to react with 5T4 in wild-type MCF7 cells in Western blot with loss of signal observed in TPBG knockout cell line ab269499 (knockout cell lysate ab269661). Wild-type MCF7 and TPBG knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in before incubation with ab129058 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (ab129058) at 1/1000 dilution

Lane 1:

Wild-type MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TPBG knockout MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TPBG knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-tpbg-knockout-mcf7-cell-line-ab269499'>ab269499</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 85 kDa

false

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution

Lane 1:

HT-1376 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 106 kDa,113 kDa,119 kDa,42 kDa,46 kDa,72 kDa,85 kDa,92 kDa,94 kDa,98 kDa

Observed band size: 106 kDa,110 kDa,42 kDa,85 kDa

false

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)
  • WB

Unknown

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (ab129058) at 1/1000 dilution

Lane 1:

HT 1376 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Lane 4:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution

All lanes:

Rat brain at 20 µg

Secondary

All lanes:

HRP goat-anti rabbit (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)
  • WB

Lab

Western blot - Anti-5T4 antibody [EPR5530] (AB129058)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution

All lanes:

Rat brain at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

false

  • Carrier free

    Anti-5T4 antibody [EPR5530] - BSA and Azide free

  • HRP

    HRP Anti-5T4 antibody [EPR5530]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5530

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5T4 protein also known as trophoblast glycoprotein or B3F1 is a cell surface antigen with a mass of approximately 72 kDa. It is encoded by the TPBG gene and expresses mainly in embryonic tissues and various cancers with limited expression in normal adult tissues. The 5T4 protein is characterized by a densely glycosylated structure contributing to its role in cellular processes like adhesion and migration.
Biological function summary

5T4 is involved in processes associated with embryonic development and cellular mobility. It contributes to the epithelial-to-mesenchymal transition (EMT) which is a critical process in both normal development and cancer metastasis. Although 5T4 is not part of a known specific complex its glycoprotein nature allows interaction with the extracellular matrix and other cell surface proteins influencing cell behavior and morphology.

Pathways

5T4 plays a part in signaling pathways involved in cell migration and invasion such as the Wnt/β-catenin pathway and TGF-β signaling. In the context of the Wnt/β-catenin pathway 5T4 interacts with proteins like β-catenin influencing cell proliferation and survival. Through its involvement in TGF-β signaling 5T4 can impact cell differentiation processes and regulatory mechanisms often linking to other proteins such as SMAD family members within these pathways.

5T4 is associated with cancer particularly in its role in promoting metastasis. Its expression is notably increased in several carcinomas including breast and colorectal cancers. The elevated levels of 5T4 in tumors correlate with poorer prognosis making it a potential target for cancer therapies. Additionally 5T4 has connections to proteins such as MMPs (matrix metalloproteinases) which facilitate tissue remodeling and metastatic spread highlighting its significance in cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
See full target information TPBG
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 10: PubMed36323434

2022

Intravenous administration of viral vectors expressing prostate cancer antigens enhances the magnitude and functionality of CD8+ T cell responses.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Vardeu,Charlotte Davis,Ian McDonald,Guilherme Stahlberg,Barsha Thapa,Kinga Piotrowska,Margaret A Marshall,Thomas Evans,Vicky Wheeler,Sarah Sebastian,Katie Anderson

Investigative ophthalmology & visual science 63:13 PubMed35551574

2022

Identification of TPBG-Expressing Amacrine Cells in DAT-tdTomato Mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Wanjing Huang,Qiang Xu,Feng Liu,Jing Su,Dongchang Xiao,Lei Tang,Zhao-Zhe Hao,Ruifeng Liu,Kangjian Xiang,Yalan Bi,Zhichao Miao,Xialin Liu,Yizhi Liu,Sheng Liu

Journal of hematology & oncology 13:24 PubMed32216806

2020

HNF4A-AS1/hnRNPU/CTCF axis as a therapeutic target for aerobic glycolysis and neuroblastoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Huajie Song,Dan Li,Xiaojing Wang,Erhu Fang,Feng Yang,Anpei Hu,Jianqun Wang,Yanhua Guo,Yang Liu,Hongjun Li,Yajun Chen,Kai Huang,Liduan Zheng,Qiangsong Tong

The Journal of comparative neurology 528:1660-1671 PubMed31891182

2020

Expression and distribution of trophoblast glycoprotein in the mouse retina.

Applications

Unspecified application

Species

Unspecified reactive species

Colin M Wakeham,Gaoying Ren,Catherine W Morgans

Journal of proteomics 206:103423 PubMed31255707

2019

Identification of PKCα-dependent phosphoproteins in mouse retina.

Applications

Unspecified application

Species

Unspecified reactive species

Colin M Wakeham,Phillip A Wilmarth,Jennifer M Cunliffe,John E Klimek,Gaoying Ren,Larry L David,Catherine W Morgans

Experimental cell research 364:175-183 PubMed29408206

2018

The 5T4 oncofetal glycoprotein does not act as a general organizer of the CXCL12 system in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Puchert,Christian Koch,Jürgen Engele

Clinical cancer research : an official journal of the American Association for Cancer Research 22:383-94 PubMed26319086

2015

Enhanced Antitumor Activity of an Anti-5T4 Antibody-Drug Conjugate in Combination with PI3K/mTOR inhibitors or Taxanes.

Applications

WB

Species

Human

Boris Shor,Jennifer Kahler,Maureen Dougher,Jane Xu,Michelle Mack,Ed Rosfjord,Fang Wang,Eugene Melamud,Puja Sapra
View all publications
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Product promise

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