AB189508

Anti-5T4 antibody [EPR5530] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal 5T4 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

5T4, TPBG, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, M6P1, Wnt-activated inhibitory factor 1, 5T4 oncotrophoblast glycoprotein, WAIF1

8 Images

Lab

Immunoprecipitation - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using ab129058, the same antibody clone in a different buffer formulation.

ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>)

Predicted band size: 46 kDa

false

Lab

Immunoprecipitation - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using ab129058, the same antibody clone in a different buffer formulation.

ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>)

Predicted band size: 46 kDa

false

Lab

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using ab129058, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>) at 1/5000 dilution

Lane 1:

HT-1376 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

false

Lab

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using the same antibody clone in a different buffer formulation (ab129058).

Lanes 1 - 4 : Merged signal (red and green). Green - ab129058 observed at 85 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab129058 was shown to react with 5T4 in wild-type MCF7 cells in Western blot with loss of signal observed in TPBG knockout cell line ab269499 (knockout cell lysate ab269661). Wild-type MCF7 and TPBG knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in before incubation with ab129058 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TPBG knockout MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TPBG knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-tpbg-knockout-mcf7-cell-line-ab269499'>ab269499</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 85 kDa

false

Lab

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using ab129058, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>) at 1/2000 dilution

Lane 1:

HT-1376 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 106 kDa,113 kDa,119 kDa,42 kDa,46 kDa,72 kDa,85 kDa,92 kDa,94 kDa,98 kDa

Observed band size: 106 kDa,110 kDa,42 kDa,85 kDa

false

Unknown

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using ab129058, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>) at 1/1000 dilution

Lane 1:

HT 1376 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Lane 4:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 46 kDa

false

Lab

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using ab129058, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>) at 1/5000 dilution

All lanes:

Rat brain at 20 µg

Secondary

All lanes:

HRP goat-anti rabbit (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

false

Lab

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

This data was developed using ab129058, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-5T4 antibody [EPR5530] (<a href='/en-us/products/primary-antibodies/5t4-antibody-epr5530-ab129058'>ab129058</a>) at 1/2000 dilution

All lanes:

Rat brain at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 46 kDa

false

Unconjugated

Anti-5T4 antibody [EPR5530]
HRP

HRP Anti-5T4 antibody [EPR5530]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5530

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab189508 is the carrier-free version of ab129058.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5T4 protein also known as trophoblast glycoprotein or B3F1 is a cell surface antigen with a mass of approximately 72 kDa. It is encoded by the TPBG gene and expresses mainly in embryonic tissues and various cancers with limited expression in normal adult tissues. The 5T4 protein is characterized by a densely glycosylated structure contributing to its role in cellular processes like adhesion and migration.

Biological function summary

5T4 is involved in processes associated with embryonic development and cellular mobility. It contributes to the epithelial-to-mesenchymal transition (EMT) which is a critical process in both normal development and cancer metastasis. Although 5T4 is not part of a known specific complex its glycoprotein nature allows interaction with the extracellular matrix and other cell surface proteins influencing cell behavior and morphology.

Pathways

5T4 plays a part in signaling pathways involved in cell migration and invasion such as the Wnt/β-catenin pathway and TGF-β signaling. In the context of the Wnt/β-catenin pathway 5T4 interacts with proteins like β-catenin influencing cell proliferation and survival. Through its involvement in TGF-β signaling 5T4 can impact cell differentiation processes and regulatory mechanisms often linking to other proteins such as SMAD family members within these pathways.

5T4 is associated with cancer particularly in its role in promoting metastasis. Its expression is notably increased in several carcinomas including breast and colorectal cancers. The elevated levels of 5T4 in tumors correlate with poorer prognosis making it a potential target for cancer therapies. Additionally 5T4 has connections to proteins such as MMPs (matrix metalloproteinases) which facilitate tissue remodeling and metastatic spread highlighting its significance in cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

See full target information TPBG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 13:e71009 PubMed40994455

2025

Screening Based on Structural and Biological Verification of Stachyose as a PPARγ-Modulating Ligand for the Treatment of Non-Alcoholic Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Binbo Fang,Mengyuan Li,Feng Jiang,Weisong Dong,Weizhi Zhang,Lifan Lin,Yongheng Bai,Jianjian Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-5T4 antibody [EPR5530] - BSA and Azide free

Immunoprecipitation - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Immunoprecipitation - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Western blot - Anti-5T4 antibody [EPR5530] - BSA and Azide free (AB189508)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise