AB275863

Anti-67kDa Laminin Receptor antibody [EPR23768-137] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 67kDa Laminin Receptor antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

LAMBR, LAMR1, RPSA, Small ribosomal subunit protein uS2, 37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 40S ribosomal protein SA, 67 kDa laminin receptor, Colon carcinoma laminin-binding protein, Laminin receptor 1, Laminin-binding protein precursor p40, Multidrug resistance-associated protein MGr1-Ag, NEM/1CHD4, 37LRP, LRP/LR, 67LR, LamR, LBP/p40

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Sandwich ELISA - Anti-67kDa Laminin Receptor antibody [EPR23768-137] - BSA and Azide free (Capture) (AB275863)

sELISA

Lab

Sandwich ELISA - Anti-67kDa Laminin Receptor antibody [EPR23768-137] - BSA and Azide free (Capture) (AB275863)

Sandwich ELISA of ab253550 with the capture antibody ab275863 dilution at 2 µg/mL and detector antibody ab275865 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23768-137

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab275863 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RPSA. The recommended pair for sandwich ELISA is:
Capture: ab275863, Human RPSA Capture Antibody (unconjugated)
Detector: ab275865, Human RPSA Detector Antibody (unconjugated)
The reference range value is 78.13 - 5000 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 67kDa Laminin Receptor also known as the laminin binding protein or protein laminin functions as a receptor for laminin an important component of the extracellular matrix. This receptor is expressed in many tissue types serving an important mechanical role by mediating the adhesion and migration of cells. The receptor anchors cells to laminin molecules thereby influencing cell differentiation and proliferation. The molecular weight of the laminin receptor is approximately 67 kilodaltons which signifies it as a critical player in various cellular processes.

Biological function summary

This receptor engages in cellular interactions that are essential for maintaining the integrity and architecture of tissues. It often associates with other proteins to form complexes which further strengthen its functional capabilities. By anchoring laminin to the cell surface it regulates cell signaling pathways that influence cell motility invasion and survival. These interactions suggest that the 67kDa Laminin Receptor's function goes beyond simple adhesion impacting cellular responsiveness to external matrix cues.

Pathways

The receptor influences the MAPK and PI3K/Akt signaling pathways which are significant in cell survival and proliferation. Through such pathways it interacts with numerous proteins including integrins and fibronectin receptors facilitating orchestrated cell signaling. This connectivity highlights the receptor's role in cellular communication networks contributing to dynamic processes like wound healing and angiogenesis.

Aberrant expression of the 67kDa Laminin Receptor relates to conditions such as cancer and Alzheimer's disease. In cancer its overexpression links to increased tumor invasiveness likely due to enhanced cell motility mediated through integrin interactions. In Alzheimer's disease its relationship with amyloid precursor protein influences plaque formation exacerbating disease progression. Understanding these connections highlights potential therapeutic targets for disease intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.. (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9.. (Microbial infection) Acts as a receptor for the Dengue virus.. (Microbial infection) Acts as a receptor for the Sindbis virus.. (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus.. (Microbial infection) Acts as a receptor for the pathogenic prion protein.. (Microbial infection) Acts as a receptor for bacteria.

See full target information RPSA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-67kDa Laminin Receptor antibody [EPR23768-137] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-67kDa Laminin Receptor antibody [EPR23768-137] - BSA and Azide free (Capture) (AB275863)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise