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Rabbit Recombinant Monoclonal 67kDa Laminin Receptor antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (AB248653), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (AB248653), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (AB248653), expandable thumbnail
  • OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (AB248653), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Predicted
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9. (Microbial infection) Acts as a receptor for the Dengue virus. (Microbial infection) Acts as a receptor for the Sindbis virus. (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus. (Microbial infection) Acts as a receptor for the pathogenic prion protein. (Microbial infection) Acts as a receptor for bacteria.

Alternative names

LAMBR, LAMR1, RPSA, Small ribosomal subunit protein uS2, 37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 40S ribosomal protein SA, 67 kDa laminin receptor, Colon carcinoma laminin-binding protein, Laminin receptor 1, Laminin-binding protein precursor p40, Multidrug resistance-associated protein MGr1-Ag, NEM/1CHD4, 37LRP, LRP/LR, 67LR, LamR, LBP/p40

Recommended products

Rabbit Recombinant Monoclonal 67kDa Laminin Receptor antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8468
Purification technique
Affinity purification Protein A
Dissociation constant
6.7 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248653 is the carrier-free version of Anti-67kDa Laminin Receptor antibody [EPR8468] ab133775.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The 67kDa Laminin Receptor also known as the laminin binding protein or protein laminin functions as a receptor for laminin an important component of the extracellular matrix. This receptor is expressed in many tissue types serving an important mechanical role by mediating the adhesion and migration of cells. The receptor anchors cells to laminin molecules thereby influencing cell differentiation and proliferation. The molecular weight of the laminin receptor is approximately 67 kilodaltons which signifies it as a critical player in various cellular processes.

Biological function summary

This receptor engages in cellular interactions that are essential for maintaining the integrity and architecture of tissues. It often associates with other proteins to form complexes which further strengthen its functional capabilities. By anchoring laminin to the cell surface it regulates cell signaling pathways that influence cell motility invasion and survival. These interactions suggest that the 67kDa Laminin Receptor's function goes beyond simple adhesion impacting cellular responsiveness to external matrix cues.

Pathways

The receptor influences the MAPK and PI3K/Akt signaling pathways which are significant in cell survival and proliferation. Through such pathways it interacts with numerous proteins including integrins and fibronectin receptors facilitating orchestrated cell signaling. This connectivity highlights the receptor's role in cellular communication networks contributing to dynamic processes like wound healing and angiogenesis.

Associated diseases and disorders

Aberrant expression of the 67kDa Laminin Receptor relates to conditions such as cancer and Alzheimer's disease. In cancer its overexpression links to increased tumor invasiveness likely due to enhanced cell motility mediated through integrin interactions. In Alzheimer's disease its relationship with amyloid precursor protein influences plaque formation exacerbating disease progression. Understanding these connections highlights potential therapeutic targets for disease intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653), expandable thumbnail

    Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)

    This data was developed using Anti-67kDa Laminin Receptor antibody [EPR8468] ab133775, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (Anti-67kDa Laminin Receptor antibody [EPR8468] ab133775) at 1/2000 dilution

    Lane 1: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) at 15 µg

    Lane 4: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 43 kDa

    Exposure time: 60s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)

    This data was developed using Anti-67kDa Laminin Receptor antibody [EPR8468] ab133775, the same antibody clone in a different buffer formulation.Anti-67kDa Laminin Receptor antibody [EPR8468] ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human brain tissue using immunohistochemical analysis. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)

    This data was developed using Anti-67kDa Laminin Receptor antibody [EPR8468] ab133775, the same antibody clone in a different buffer formulation.Anti-67kDa Laminin Receptor antibody [EPR8468] ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human kidney tissue using immunohistochemical analysis. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653), expandable thumbnail

    OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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