Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

Mouse Monoclonal 67kDa Laminin Receptor antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RPSA.

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Images

Flow Cytometry - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (AB268200), expandable thumbnail

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Full Length Protein corresponding to Human RPSA. Database link P08865

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	Flow Cyt	WB	Protein Array	IHC-P
Human	Tested	Tested	Tested	Expected	Tested
Recombinant full length protein - Human	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1-2 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1-2 µg/mL	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1-2 µg/mL	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1-2 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information RPSA

Function

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9. (Microbial infection) Acts as a receptor for the Dengue virus. (Microbial infection) Acts as a receptor for the Sindbis virus. (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus. (Microbial infection) Acts as a receptor for the pathogenic prion protein. (Microbial infection) Acts as a receptor for bacteria.

Alternative names

LAMBR, LAMR1, RPSA, Small ribosomal subunit protein uS2, 37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 40S ribosomal protein SA, 67 kDa laminin receptor, Colon carcinoma laminin-binding protein, Laminin receptor 1, Laminin-binding protein precursor p40, Multidrug resistance-associated protein MGr1-Ag, NEM/1CHD4, 37LRP, LRP/LR, 67LR, LamR, LBP/p40

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Full Length Protein corresponding to Human RPSA. Database link P08865
Carrier free: Yes
Clone number: RPSA/2699
Purification technique: Affinity purification Protein A/G
Light chain type: kappa
Concentration
Purification notes: Purified from bioreactor concentrate.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

ab268200 is the carrier-free version of Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 67kDa Laminin Receptor also known as the laminin binding protein or protein laminin functions as a receptor for laminin an important component of the extracellular matrix. This receptor is expressed in many tissue types serving an important mechanical role by mediating the adhesion and migration of cells. The receptor anchors cells to laminin molecules thereby influencing cell differentiation and proliferation. The molecular weight of the laminin receptor is approximately 67 kilodaltons which signifies it as a critical player in various cellular processes.

Biological function summary

This receptor engages in cellular interactions that are essential for maintaining the integrity and architecture of tissues. It often associates with other proteins to form complexes which further strengthen its functional capabilities. By anchoring laminin to the cell surface it regulates cell signaling pathways that influence cell motility invasion and survival. These interactions suggest that the 67kDa Laminin Receptor's function goes beyond simple adhesion impacting cellular responsiveness to external matrix cues.

Pathways

The receptor influences the MAPK and PI3K/Akt signaling pathways which are significant in cell survival and proliferation. Through such pathways it interacts with numerous proteins including integrins and fibronectin receptors facilitating orchestrated cell signaling. This connectivity highlights the receptor's role in cellular communication networks contributing to dynamic processes like wound healing and angiogenesis.

Associated diseases and disorders

Aberrant expression of the 67kDa Laminin Receptor relates to conditions such as cancer and Alzheimer's disease. In cancer its overexpression links to increased tumor invasiveness likely due to enhanced cell motility mediated through integrin interactions. In Alzheimer's disease its relationship with amyloid precursor protein influences plaque formation exacerbating disease progression. Understanding these connections highlights potential therapeutic targets for disease intervention.

Product promise

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10 product images

Flow Cytometry - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Flow Cytometric analysis of paraformaldehyde-fixed Raji (Human Burkitt's lymphoma cell line) cells labelling 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at at 2 μg/ml followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Formalin-fixed, paraffin-embedded human basal cell carcinoma tissue stained for 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
Protein Array - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Protein Array containing more than 19,000 full-length human proteins using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079).
Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at 2 μg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
Western blot - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
All lanes: Western blot - Anti-67kDa Laminin Receptor antibody [RPSA/2699] (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079) at 2 µg/mL
Lane 1: THP-1 (Human monocytic leukemia cell line) cell lysate
Lane 2: U937 (Human histiocytic lymphoma cell line) cell lysate
Lane 3: SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
Lane 4: A431 (Human epidermoid carcinoma cell line) cell lysate
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Immunofluorescent staining of paraformaldehyde-fixed Raji (Human Burkitt's lymphoma cell line) cells stained for 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at 2 μg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Formalin-fixed, paraffin-embedded human cervix carcinoma tissue stained for 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for 67kDa Laminin Receptor using Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-67kDa Laminin Receptor antibody [RPSA/2699] ab268079)