Anti-68kDa Neurofilament/NF-L antibody [1B11]

• IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [1B11] (AB315188)

Immunostaining of a coronal section of the spinal cord of a rat given a midline C4 contusion injury three days previously. Sections were stained with RPCA-NF-L-ct in red and ab315188 in green. ab315188 stains prominent aggregates of material concentrated in the lateral funiculi and the dorsal columns but seen in lesser amounts throughout the section. These are degenerating and degenerated axons damaged by the C4 lesion. The RPCA-NF-L-ct antibody binds the C-terminal “tail” region of NF-L which is absent or destroyed during degeneration, so the ab315188 positive profiles are largely negative for RPCA-NF-L-ct.

• WB

Supplier Data

Western blot - Anti-68kDa Neurofilament/NF-L antibody [1B11] (AB315188)

Western blot analysis of different tissue lysates using ab315188, dilution 1 : 20,000 in green : [1] protein standard, [2] rat brain, [3] mouse brain and [4] cow cerebellum. Strong band at about 68-70kDa corresponds to NF-L protein, with the cow protein appearing slightly larger in molecular size as expected. Low molecular weight bands detected in cow brain sample are likely post mortem proteolytic forms of NF-L.

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [1B11] (ab315188) at 1/20000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Mouse brain tissue lysate

Lane 4:

Cow cerebellum tissue lysate

Observed band size: 68-70 kDa

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