Anti-68kDa Neurofilament/NF-L antibody [1D44]

• IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [1D44] (AB315189)

A Keyence merged image of an entire coronal section from a of rat given a contusion injury three days previously and stained for RPCA-NF-L-ct in red and ab315189 in green. ab315189 positive profiles are particularly obvious in the dorsal columns, corticospinal tracts and rubrospinal tracts, less abundant in the lateral and ventral funuculi and least abundant but not totally absent in the spinal cord gray matter. Full details of these findings are described in a pending peer-reviewed research report and in our recent BioRχiv article.

• WB

Supplier Data

Western blot - Anti-68kDa Neurofilament/NF-L antibody [1D44] (AB315189)

Western blot analysis of different tissue lysates using ab315189, dilution 1 : 1,000 in green : Lane [1] protein standard with standards of indicated molecular weight and homogenates of [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Strong band at about 68kDa corresponds to full length NF-L protein. Lower molecular weight bands detected in the samples are presumably proteolytic forms of NF-L.

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [1D44] (ab315189) at 1/1000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Rat spinal cord tissue lysate

Lane 4:

Mouse brain tissue lysate

Lane 5:

Mouse spinal cord tissue lysate

Lane 6:

Cow spinal cord tissue lysate

Lane 7:

Pig spinal cord tissue lysate

Observed band size: 68 kDa

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