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Mouse Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB, IHC-FoFr and reacts with Rat, Cow samples.

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Images

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [6H63] (AB315187), expandable thumbnail
  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [6H63] (AB315187), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7 - 8
Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.97% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FoFr
Rat
Tested
Tested
Cow
Tested
Expected

Tested
Tested

Species
Rat, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

Recommended products

Mouse Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB, IHC-FoFr and reacts with Rat, Cow samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
6H63
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Associated diseases and disorders

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [6H63] (ab315187), expandable thumbnail

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [6H63] (ab315187)

    Immunostaining of a coronal section of the spinal cord of a rat given a midline C4 contusion injury three days previously. Sections were stained with RPCA-NF-L-ct (red) and ab315187 in green. ab315187 stains prominent aggregates of material concentrated in the lateral funiculi and the dorsal columns but seen in lesser amounts throughout the section. These are degenerating and degenerated axons damaged by the C4 lesion. The RPCA-NF-L-ct antibody binds the C-terminal “tail” region of NF-L which is absent or destroyed during degeneration, so the ab315187 positive profiles are largely negative for RPCA-NF-L-ct.

  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [6H63] (ab315187), expandable thumbnail

    Western blot - Anti-68kDa Neurofilament/NF-L antibody [6H63] (ab315187)

    Western blotting of ab315187 on crude CNS homogenates. Lane 1 shows molecular weight standards of indicated size. Lane 2 shows western blot of homogenate of E20 rat spinal cord, lane 3 homogenate of adult rat spinal cord and lane 4 shows homogenate of cow spinal cord extract. The ab315187 antibody binds the denatured forms of NF-L with apparent molecular weight 68-70kDa as immobilized on western blotting membranes. Lower molecular weight bands under the major band are proteolytic fragments of NF-L.

    All lanes: Western blot - Anti-68kDa Neurofilament/NF-L antibody [6H63] (ab315187)

    Lane 2: Homogenate of E20 rat spinal cord

    Lane 3: Homogenate of adult rat spinal cord

    Lane 4: Homogenate of cow spinal cord extract

    Observed band size: 68-70 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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