Anti-68kDa Neurofilament/NF-L antibody [6H63]

• IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [6H63] (AB315187)

Immunostaining of a coronal section of the spinal cord of a rat given a midline C4 contusion injury three days previously. Sections were stained with RPCA-NF-L-ct (red) and ab315187 in green. ab315187 stains prominent aggregates of material concentrated in the lateral funiculi and the dorsal columns but seen in lesser amounts throughout the section. These are degenerating and degenerated axons damaged by the C4 lesion. The RPCA-NF-L-ct antibody binds the C-terminal “tail” region of NF-L which is absent or destroyed during degeneration, so the ab315187 positive profiles are largely negative for RPCA-NF-L-ct.

• WB

Supplier Data

Western blot - Anti-68kDa Neurofilament/NF-L antibody [6H63] (AB315187)

Western blotting of ab315187 on crude CNS homogenates. Lane 1 shows molecular weight standards of indicated size. Lane 2 shows western blot of homogenate of E20 rat spinal cord, lane 3 homogenate of adult rat spinal cord and lane 4 shows homogenate of cow spinal cord extract. The ab315187 antibody binds the denatured forms of NF-L with apparent molecular weight 68-70kDa as immobilized on western blotting membranes. Lower molecular weight bands under the major band are proteolytic fragments of NF-L.

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [6H63] (ab315187)

Lane 2:

Homogenate of E20 rat spinal cord

Lane 3:

Homogenate of adult rat spinal cord

Lane 4:

Homogenate of cow spinal cord extract

Observed band size: 68-70 kDa

false