AB134460

Anti-68kDa Neurofilament/NF-L antibody

Name: Anti-68kDa Neurofilament/NF-L antibody (ab134460) | Abcam
Brand: Abcam Limited
SKU: AB134460
Price: 545 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(2 Publications)

Chicken Polyclonal 68kDa Neurofilament/NF-L antibody. Suitable for IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NEFL conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/Immunofluorescence analysis of Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde. NFL immunoreactivity is red and yellow, Blue - DAPI nuclear stain.

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunohistochemical analysis of adult mouse brain labelling 68kDa Neurofilament/NF-L in axons in the white matter of the cerebellum with ab134460 at 1/1000 dilution (brown staining).

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Mouse

Applications

ICC/IF, IHC-FoFr

applications

Immunogen

Synthetic Peptide within Human NEFL conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07196

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/1000 - 1/2000", "IHCFoFr-species-notes": "<p>Use 2% paraformaldehyde-fixed tissues.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "<p>Use 2% paraformaldehyde-fixed cells.</p>" } } }

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Product details

Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging biology 1: PubMed38500536

2024

Early-Onset Hearing Loss in Mouse Models of Alzheimer's Disease and Increased DNA Damage in the Cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Hyeon Park,Burcin Duan Sahbaz,Komal Pekhale,Xixia Chu,Mustafa N Okur,Mhamed Grati,Kevin Isgrig,Wade Chien,Elena Chrysostomou,Lauren Sullivan,Deborah L Croteau,Uri Manor,Vilhelm A Bohr

Nature 605:152-159 PubMed35477759

2022

Neuroimmune cardiovascular interfaces control atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarajo K Mohanta,Li Peng,Yuanfang Li,Shu Lu,Ting Sun,Lorenzo Carnevale,Marialuisa Perrotta,Zhe Ma,Benjamin Förstera,Karen Stanic,Chuankai Zhang,Xi Zhang,Piotr Szczepaniak,Mariaelvy Bianchini,Borhan R Saeed,Raimondo Carnevale,Desheng Hu,Ryszard Nosalski,Fabio Pallante,Michael Beer,Donato Santovito,Ali Ertürk,Thomas C Mettenleiter,Barbara G Klupp,Remco T A Megens,Sabine Steffens,Jaroslav Pelisek,Hans-Henning Eckstein,Robert Kleemann,Livia Habenicht,Ziad Mallat,Jean-Baptiste Michel,Jürgen Bernhagen,Martin Dichgans,Giuseppe D'Agostino,Tomasz J Guzik,Peder S Olofsson,Changjun Yin,Christian Weber,Giuseppe Lembo,Daniela Carnevale,Andreas J R Habenicht

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-68kDa Neurofilament/NF-L antibody

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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