AB72997

Anti-68kDa Neurofilament/NF-L antibody

Name: Anti-68kDa Neurofilament/NF-L antibody (ab72997) | Abcam
Brand: Abcam Limited
SKU: AB72997
Price: 545 USD
Availability: InStock
Rating: 4 (11 reviews)

(11 Reviews)

(16 Publications)

Chicken Polyclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB and reacts with Rat samples. Cited in 16 publications. Immunogen corresponding to Tissue preparation containing NEFL protein.

View Alternative Names

Nf68, Nfl, Nefl, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

1 Images

Unknown

Western blot - Anti-68kDa Neurofilament/NF-L antibody (AB72997)

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody (ab72997) at 1/10000 dilution

All lanes:

Rat cerebral cortex lysate

Predicted band size: 62 kDa

Observed band size: 68 kDa

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Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Tissue preparation containing NEFL protein. The exact immunogen used to generate this antibody is proprietary information.

P02548

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Storage buffer

Preservative: 0.065% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information Nefl

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 10:54 PubMed38769343

2024

LncRNA TubAR complexes with TUBB4A and TUBA1A to promote microtubule assembly and maintain myelination.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Liang,Meng Gong,Zhikai Wang,Jie Wang,Weiwei Guo,Aoling Cai,Zhenye Yang,Xing Liu,Fuqiang Xu,Wei Xiong,Chuanhai Fu,Xiangting Wang

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 33:2116-2128 PubMed38436876

2024

Development of a novel animal model of lumbar vertebral endplate lesion by intervertebral disk injection of monosodium iodoacetate in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiaki Maruyama,Toshio Nakamae,Naosuke Kamei,Taiki Morisako,Kazuto Nakao,Fadlyansyah Farid,Hiroki Fukui,Nobuo Adachi

Journal of cell science 136: PubMed37642648

2023

Schwann cells are axo-protective after injury irrespective of myelination status in mouse Schwann cell-neuron cocultures.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Mutschler,Shaline V Fazal,Nathalie Schumacher,Andrea Loreto,Michael P Coleman,Peter Arthur-Farraj

Frontiers in cellular neuroscience 17:1158388 PubMed37091921

2023

SARM1 detection in myelinating glia: / is dispensable for PNS and CNS myelination in zebrafish and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shaline V Fazal,Clara Mutschler,Civia Z Chen,Mark Turmaine,Chiung-Ya Chen,Yi-Ping Hsueh,Andrea Ibañez-Grau,Andrea Loreto,Angeles Casillas-Bajo,Hugo Cabedo,Robin J M Franklin,Roger A Barker,Kelly R Monk,Benjamin J Steventon,Michael P Coleman,Jose A Gomez-Sanchez,Peter Arthur-Farraj

Virologica Sinica 37:591-600 PubMed35688418

2022

Phospho-proteomics identifies a critical role of ATF2 in pseudorabies virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Fang Jiang,Ren-Qi Wang,Chao-Yue Guo,Ke Zheng,Hai-Long Liu,Le Su,Sheng-Song Xie,Huan-Chun Chen,Zheng-Fei Liu

Aging 13:18051-18093 PubMed34319911

2021

Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system.

Applications

Unspecified application

Species

Unspecified reactive species

Sílvia Gras,Alba Blasco,Guillem Mòdol-Caballero,Olga Tarabal,Anna Casanovas,Lídia Piedrafita,Alejandro Barranco,Tapas Das,Ricardo Rueda,Suzette L Pereira,Xavier Navarro,Josep E Esquerda,Jordi Calderó

Biomolecules 11: PubMed33921657

2021

Central Alteration in Peripheral Neuropathy of Trembler-J Mice: Hippocampal pmp22 Expression and Behavioral Profile in Anxiety Tests.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Pablo Damián,Lucia Vázquez Alberdi,Lucía Canclini,Gonzalo Rosso,Silvia Olivera Bravo,Mariana Martínez,Natalia Uriarte,Paul Ruiz,Miguel Calero,María Vittoria Di Tomaso,Alejandra Kun

Journal of cachexia, sarcopenia and muscle 11:1628-1660 PubMed32691534

2020

Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Blasco,Sílvia Gras,Guillem Mòdol-Caballero,Olga Tarabal,Anna Casanovas,Lídia Piedrafita,Alejandro Barranco,Tapas Das,Suzette L Pereira,Xavier Navarro,Ricardo Rueda,Josep E Esquerda,Jordi Calderó

Journal of neuroscience research 98:869-887 PubMed31797408

2019

Ontogeny of inter-alpha inhibitor protein (IAIP) expression in human brain.

Applications

Unspecified application

Species

Unspecified reactive species

Boram Kim,Suzanne De La Monte,Virginia Hovanesian,Aparna Patra,Xiaodi Chen,Ray H Chen,Miles C Miller,Mehmet Halit Pinar,Yow-Pin Lim,Edward G Stopa,Barbara S Stonestreet

Journal of molecular and cellular cardiology 128:198-211 PubMed30742811

2019

Direct visualization of cardiac transcription factories reveals regulatory principles of nuclear architecture during pathological remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Elaheh Karbassi,Manuel Rosa-Garrido,Douglas J Chapski,Yong Wu,Shuxun Ren,Yibin Wang,Enrico Stefani,Thomas M Vondriska

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-68kDa Neurofilament/NF-L antibody

Western blot - Anti-68kDa Neurofilament/NF-L antibody (AB72997)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise