Anti-68kDa Neurofilament/NF-L antibody

• IHC-FrFl

Supplier Data

Immunohistochemistry - Free Floating - Anti-68kDa Neurofilament/NF-L antibody (AB9035)

Immunohistochemistry (Free Floating) analysis of mouse cerebellum staining 68kDa Neurofilament/NF-L with ab9035 (1/5000) in red. Costained with chicken pAb to MBP (1/5000) in green and DAPI in blue. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB9035)

ab9035 staining anti 68kDa Neurofilament/NF-L in mixed neuron/glia cultures from newborn rat brain by ICC/IF (Immunocytochemistry/immunofluorescence). Samples were incubated with primary antibody (1/500) (red) and co-stained with ab4573 for anti Peripherin (green).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB9035)

• ICC/IF

AbReview4974****

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB9035)

ab9035 at a 1/300 dilution staining rat hippocampal organotypic slice cultures by ICC/IF. The primary antibody was incubated with the cells for 120 hours (this time allows for the antibody to penetrate a layer of glial cells that grows while the slice is in culture). Bound antibody is detected using a Cy3 conjugated Indocarbocyanine.

This image is courtesy of an Abreview submitted by Dr Luis Craveiro

• WB

Supplier Data

Western blot - Anti-68kDa Neurofilament/NF-L antibody (AB9035)

Lane 1:

Protein ladder

Lanes 2 - 5:

Western blot - Anti-68kDa Neurofilament/NF-L antibody (ab9035) at 1/20000 dilution

Lane 2:

Rat brain

Lane 3:

Rat spinal cord

Lane 4:

Mouse brain

Lane 5:

Mouse spinal cord

Predicted band size: 62 kDa

Observed band size: 68 kDa

false