Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for I-ELISA, IP, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
I-ELISA | IP | WB | ICC/IF | IHC-Fr | IHC-P | |
---|---|---|---|---|---|---|
Human | Tested | Expected | Tested | Expected | Expected | Tested |
Mouse | Expected | Tested | Tested | Tested | Tested | Tested |
Rat | Expected | Expected | Tested | Expected | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein
Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for I-ELISA, IP, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR22035-112
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.
The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.
Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.
Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining in mouse cerebellum (PMID: 17598884) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling Neurofilament/NF-L with purified ab223343 at 1/50 (9.4μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 antibody [HM-2]; Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Blocking and dilution buffer: 5% NFDM/TBST.
Negative control: Mouse liver (PMID: 2120242).
All lanes: Western blot - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] (ab223343) at 1/1000 dilution
Lane 1: Human brain lysate at 20 µg
Lane 2: Mouse brain lysate at 20 µg
Lane 3: Rat brain lysate at 20 µg
Lane 4: Mouse liver lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa
Exposure time: 3min
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in rat cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
68kDa Neurofilament/NF-L was immunoprecipitated from 0.35 mg of mouse brain lysate with ab223343 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223343 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 μg (Input).
Lane 2: ab223343 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab223343 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] (ab223343)
Predicted band size: 62 kDa
Observed band size: 68 kDa
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining in rat cerebellum is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in mouse cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in human cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat liver tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. No staining in rat liver is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Negative control :Rat liver (PMID: 2120242).
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse liver tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. No staining observed in mouse liver. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Negative control: mouse liver (PMID: 2120242).
ELISA using ab223343 at 1000-0 ng/mL antibody concentrations and antigen human Neurofilament light polypeptide at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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