Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Carrier free. Suitable for I-ELISA, IP, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	I-ELISA	IP	WB	ICC/IF	IHC-Fr	IHC-P
Human	Tested	Expected	Expected	Expected	Expected	Expected
Mouse	Expected	Tested	Expected	Tested	Tested	Tested
Rat	Predicted	Predicted	Expected	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information NEFL

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

Recommended products

Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Carrier free. Suitable for I-ELISA, IP, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR22035-112
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab238420 is the carrier-free version of Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Associated diseases and disorders

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining in mouse cerebellum (PMID: 17598884) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling 68kDa Neurofilament/NF-L with purified Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/50 (9.4μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 antibody [HM-2]; Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling 68kDa Neurofilament / NF-L with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in rat cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
68kDa Neurofilament/NF-L was immunoprecipitated from 0.35 mg of mouse brain lysate with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 μg (Input).
Lane 2: Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
All lanes: Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343)
Predicted band size: 62 kDa
Observed band size: 68 kDa
Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling 68kDa Neurofilament/NF-L with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining in rat cerebellum is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in mouse cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling 68kDa Neurofilament/NF-L with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in human cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat liver tissue labeling 68kDa Neurofilament/NF-L with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. No staining in rat liver is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Negative control :Rat liver (PMID: 2120242).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse liver tissue labeling 68kDa Neurofilament/NF-L with Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. No staining observed in mouse liver. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Negative control: mouse liver (PMID: 2120242).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).
Indirect ELISA - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343).

ELISA using Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] ab223343 at 1000-0 ng/mL antibody concentrations and antigen human Neurofilament light polypeptide at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

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Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free

Images

Publications

Key facts

Immunogen

Reactivity data

I-ELISA

TestedTested

ExpectedExpected

PredictedPredicted

IP

TestedTested

ExpectedExpected

PredictedPredicted

WB

ExpectedExpected

ICC/IF

TestedTested

ExpectedExpected

PredictedPredicted

IHC-Fr

TestedTested

ExpectedExpected

PredictedPredicted

IHC-P

TestedTested

ExpectedExpected

PredictedPredicted

Associated Products

8 products for Alternative Product

3 products for Alternative Version

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

10 product images

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Indirect ELISA - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Downloads

Product protocols

Tested
Tested

Expected
Expected

Predicted
Predicted

Tested
Tested

Expected
Expected

Predicted
Predicted

Expected
Expected

Tested
Tested

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Expected

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Tested

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Tested

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