Mouse Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Images
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--western-blot-img4764.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87520.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441)")
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--western-blot-img41784.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108914.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113846.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (AB273441)")
Publications
Filter by
- International endodontic journal 57:37-492023Sensory nerves, but not sympathetic nerves, promote reparative dentine formation after dentine injury via CGRP-mediated angiogenesis: An in vivo study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoning Zhan,Minchun Huang,Junyang Chen,Yanli Lu,Xiaojun Yang,Jin HouPubMed 37874659
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Tested | Not recommended | Tested |
| Rat | Not recommended | Tested | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein
Recommended products
Mouse Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- Nfl21
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.
- Biological function summary
The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.
- Pathways
Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.
- Associated diseases and disorders
Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--western-blot-img4764.jpg "Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)")
Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 37 seconds; Lane 2: 3 minutes.
All lanes: Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441) at 1/1000 dilution
Lane 1: SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 2: Human cerebrospinal fluid at 40 µL
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 62 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87520.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--western-blot-img41784.jpg "Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)")
Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)
Negative control: liver (PMID: 2120242).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 10 seconds; Lane 3-5: 37 seconds.
All lanes: Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441) at 1/1000 dilution
Lane 1: Mouse hippocampus tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat hippocampus tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution
Predicted band size: 62 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108914.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-antibody-nfl21-ab273441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113846.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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