Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (AB273479)

This data was developed using ab273441 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (AB273479)

This data was developed using ab273441 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (AB273479)

This data was developed using ab273441 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (AB273479)

This data was developed using ab273441 the same antibody clone in a different buffer formulation.

This blot was developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 37 seconds; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (<a href='/en-us/products/primary-antibodies/68kda-neurofilament-nf-l-antibody-nfl21-ab273441'>ab273441</a>) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 2:

Human cerebrospinal fluid at 40 µL

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 62 kDa

false

• WB

Supplier Data

Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (AB273479)

This data was developed using ab273441 the same antibody clone in a different buffer formulation.

Negative control : liver (PMID : 2120242).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1-2 : 10 seconds; Lane 3-5 : 37 seconds.

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (<a href='/en-us/products/primary-antibodies/68kda-neurofilament-nf-l-antibody-nfl21-ab273441'>ab273441</a>) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat hippocampus tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 62 kDa

false