Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Human, Synthetic peptide - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425443.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425442.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425441.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814)")
![Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--western-blot-img425397.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814)")
![Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--western-blot-img425396.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (AB315814)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | I-ELISA | IP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Expected | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1000 ng/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein
Recommended products
Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Human, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28784-63
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 68kDa Neurofilament also known as NF-L (C-term) is a critical component of the cytoskeleton in neurons. It has a mass of approximately 68 kilodaltons and is expressed primarily in the central and peripheral nervous systems. As the smallest neurofilament protein NF-L plays a significant role in maintaining neuronal caliber by assembling into intermediate filaments. These networks of filaments provide structural support to neurons which is essential for proper nerve cell function and stability.
- Biological function summary
68kDa Neurofilament integrates into a complex with other neurofilament proteins such as NF-M and NF-H to form mature neurofilament structures. These filaments are necessary for axonal transport influencing the distribution of organelles and proteins along the axon. The proper function of these complexes is essential for nerve conduction and integrity impacting axonal geometry directly which is critical for sustaining efficient neuronal signaling and plasticity in the nervous system.
- Pathways
The 68kDa Neurofilament is central to the neurofilament signaling and axonal transport pathways. These pathways regulate axonal caliber and contribute to efficient neurotransmission. It interacts closely with other cytoskeletal proteins and motor proteins such as kinesin which supports the movement of cellular components within axons. This transport mechanism is important for normal neuronal function and adaptation to signals affecting the overall communication within neural networks.
- Associated diseases and disorders
Aberrations in the 68kDa Neurofilament are linked to neurodegenerative conditions like Amyotrophic Lateral Sclerosis (ALS) and Charcot-Marie-Tooth disease. In ALS misregulation of neurofilament proteins including NF-L leads to axonal transport disruptions and neuronal death. NF-L abnormalities also are associated with Charcot-Marie-Tooth disease where mutations in the gene encoding this protein disturb normal filament assembly and function contributing to peripheral neuropathy. Changes in neurofilament interactions with proteins like peripherin can exacerbate these disorders highlighting their role in disease progression and serving as potential targets for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425443.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling 68kDa Neurofilament/NF-L (C-term) with ab315814 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver (PMID: 2420946).
The section was incubated with ab315814 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425442.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease cerebrum tissue labeling 68kDa Neurofilament/NF-L (C-term) with ab315814 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on cerebrum of human Alzheimer's disease.
The section was incubated with ab315814 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425441.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling 68kDa Neurofilament/NF-L (C-term) with ab315814 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum (PMID: 34557955).
The section was incubated with ab315814 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--western-blot-img425397.jpg "Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Negative control: liver (PMID: 2420946).
The expression profile/ molecular weight observed isconsistent with what has been described in the literature (PMID: 33009465).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 1 second, lanes 2-4: 3 seconds.
All lanes: Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human spinal cord tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human alzheimers brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 75 kDa, 36 kDa
![Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--western-blot-img425396.jpg "Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Low expression: BxPC-3, ARPE-19.
The identity of the higher MW band at approximately 100 kDa is unknown.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814) at 1/1000 dilution
Lane 1: SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 50 µg with 5% NFDM/TBST
Lane 2: BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST
Lane 3: ARPE-19 (human eye epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 70 kDa, 124 kDa
Exposure time: 59s
![Indirect ELISA - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--indirect-elisa-img425532.jpg "Indirect ELISA - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Indirect ELISA - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Indirect ELISA analysis of ab315814 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Neurofilament light polypeptide 1,Human Neurofilament light polypeptide 2.
Antigen concentration: 1000 ng/ml
![Flow Cytometry (Intracellular) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--flow-cytometry-intracellular-img425517.jpg "Flow Cytometry (Intracellular) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Flow Cytometry (Intracellular) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized BxPC-3 (human pancreas adenocarcinoma epithelial cell, Left) / SK-N-BE(2) (human neuroblastoma neuroblast, Right) cells labelling 68kDa Neurofilament/NF-L (C-term) with ab315814 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: BxPC-3.
![Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunocytochemistry-immunofluorescence-img425499.jpg "Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-N-BE(2) (human neuroblastoma neuroblast) cells labelling 68kDa Neurofilament/NF-L (C-term) with ab315814 at 1/500 (2.046 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic staining in SK-N-BE(2) cell line.
Low expreesion: BxPC-3.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Indirect ELISA - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--indirect-elisa-img425532.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
![Flow Cytometry (Intracellular) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--flow-cytometry-intracellular-img425517.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
![Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814), expandable thumbnail](https://content.abcam.com/products/images/68kda-neurofilament-nf-l-c-term-antibody-epr28784-63-ab315814--immunocytochemistry-immunofluorescence-img425499.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63] (ab315814)")
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