Mouse Recombinant Monoclonal 6X His tag® antibody. C-terminal. Suitable for ELISA, WB and reacts with Tag samples.
Preservative: 0.02% Proclin 300
Constituents: 99% PBS
| ELISA | WB | |
|---|---|---|
| Tag | Expected | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
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6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Mouse Recombinant Monoclonal 6X His tag® antibody. C-terminal. Suitable for ELISA, WB and reacts with Tag samples.
Preservative: 0.02% Proclin 300
Constituents: 99% PBS
ab206500 reacts to recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins. It also has affinity for internal 6X His tag® but no affinity for 6X His tag® fused to amino terminus.
ab206500 is a recombinant chimeric antibody manufactured with variable regions (i.e. specificity) from the hybridoma 3D5, created for improved compatibility with existing reagents, assays and techniques.
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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