AB206504

Anti-6X His tag® antibody [3D5] - C-terminal

Name: Anti-6X His tag® antibody [3D5] - C-terminal (ab206504) | Abcam
Brand: Abcam Limited
SKU: AB206504
Price: 555 USD
Availability: InStock
Rating: 4 (1 reviews)

Recombinant
What is this?

(1 Review)

(3 Publications)

Rat Recombinant Monoclonal 6X His tag® antibody. C-terminal. Suitable for ELISA, WB and reacts with Tag samples. Cited in 3 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Supplier Data

Western blot - Anti-6X His tag® antibody [3D5] - C-terminal (AB206504)

This data was generated using the chimeric rabbit version of 3D5 (ab206498). A primary incubation of 1h was used and protein was detected by chemiluminescence.

All lanes:

Western blot - Anti-6X His tag® antibody [3D5] - C-terminal (<a href='/en-us/products/primary-antibodies/6x-his-tag-antibody-3d5-c-terminal-ab206498'>ab206498</a>) at 0.01 µg/mL

Lane 1:

His tag expressing plasmid transfected HEK293 cells lysate at 3 g

Lane 2:

Non-transfected HEK293 cells lysate at 3 g

Secondary

All lanes:

Anti-rabbit secondary

true

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

3D5

Isotype

IgG2b

Light chain type

kappa

Carrier free

Applications

ELISA, WB

applications

Specificity

ab206504 reacts to recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins. It also has affinity for internal 6X His tag® but no affinity for 6X His tag® fused to amino terminus.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

ab206504 is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information 6X His tag®

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:5766-5783.e25 PubMed38134874

2023

LRRC37B is a human modifier of voltage-gated sodium channels and axon excitability in cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Baptiste Libé-Philippot,Amélie Lejeune,Keimpe Wierda,Nikolaos Louros,Emir Erkol,Ine Vlaeminck,Sofie Beckers,Vaiva Gaspariunaite,Angéline Bilheu,Katerina Konstantoulea,Hajnalka Nyitrai,Matthias De Vleeschouwer,Kristel M Vennekens,Niels Vidal,Thomas W Bird,Daniela C Soto,Tom Jaspers,Maarten Dewilde,Megan Y Dennis,Frederic Rousseau,Davide Comoletti,Joost Schymkowitz,Tom Theys,Joris de Wit,Pierre Vanderhaeghen

Cell death & disease 10:864 PubMed31727880

2019

Autoantibodies to synapsin I sequestrate synapsin I and alter synaptic function.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Rocchi,Silvio Sacchetti,Antonio De Fusco,Silvia Giovedi,Barbara Parisi,Fabrizia Cesca,Markus Höltje,Klemens Ruprecht,Gudrun Ahnert-Hilger,Fabio Benfenati

BioTechniques 22:140-9 PubMed8994661

1997

Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions.

Applications

Unspecified application

Species

Unspecified reactive species

P Lindner,K Bauer,A Krebber,L Nieba,E Kremmer,C Krebber,A Honegger,B Klinger,R Mocikat,A Plückthun

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-6X His tag® antibody [3D5] - C-terminal

Western blot - Anti-6X His tag® antibody [3D5] - C-terminal (AB206504)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise