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Mouse Monoclonal 6X His tag® antibody. Suitable for IP, Flow Cyt, ELISA, Dot, WB, ICC/IF, AP and reacts with Transfected cell lysate, Tag, Transfected cell line samples. Cited in 30 publications.


Images

Immunoprecipitation - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail
  • Western blot - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail
  • Western blot - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytELISADotWBICC/IFAP
Tag
Not recommended
Expected
Expected
Expected
Not recommended
Not recommended
Expected
Transfected cell line
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Transfected cell lysate
Tested
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate

Dilution info

-

Notes

See Abreview.

Not recommended
Not recommended

Species

Tag, Transfected cell line

Dilution info

-

Notes

-

Expected
Expected

Species

Tag

Dilution info

-

Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Transfected cell lysate, Transfected cell line

Dilution info

-

Notes

-

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Transfected cell line

Dilution info

-

Notes

-

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Transfected cell line

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell lysate

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Tag, Transfected cell line

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Tag

Dilution info

-

Notes

-

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Transfected cell line

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

4 products for Alternative Version

Target data

Alternative names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag

Recommended products

Mouse Monoclonal 6X His tag® antibody. Suitable for IP, Flow Cyt, ELISA, Dot, WB, ICC/IF, AP and reacts with Transfected cell lysate, Tag, Transfected cell line samples. Cited in 30 publications.

Alternative names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

4D11

Purification technique

Affinity purification Protein G

Epitope

This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

HIS-TAG® is a trademark of EMD Biosciences, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

Associated diseases and disorders

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Vladimir Milenkovic

    Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000)

    ab5000 Immunoprecipitate in human HEK293 whole cell lysate. 25μg of cell lysate incubated with primary antibody (undiluted) and matrix (Protein G) for 16 hours at 4°C. For western blotting an undiluted HRP-Goat anti-rabbit IgG polyclonal was used. Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab

    Lane 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)

    Lane 2. IP with anti His Ab

    Lane 3. Non bound fraction

    All lanes: Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000)

  • Western blot - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail

    Western blot - Anti-6X His tag® antibody [4D11] (ab5000)

    All lanes: Western blot - Anti-6X His tag® antibody [4D11] (ab5000) at 1/1000 dilution

    All lanes: 6X His Tagged Protein at 1 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30s

  • Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Vladimir Milenkovic

    Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (ab5000)

    ab5000 staining Human HEK 293 cells transfected with CACNB3-His by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody (1/250 in 1% goat serum, 0.1% TX100, 1X PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody (1/500) was used as the secondary. DAPI was used for nuclei staining (blue).

  • Western blot - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Vladimir Milenkovic

    Western blot - Anti-6X His tag® antibody [4D11] (ab5000)

    All lanes: Western blot - Anti-6X His tag® antibody [4D11] (ab5000) at 1/2000 dilution

    Lane 1: Lysates prepared from HEK-293 cells

    Lane 2: Lysates prepared from HEK-293 cells transfected with Beta3-His (CACNB3)

    Secondary

    All lanes: HRP-conjugated goat anit-mouse IgG polyclonal at 1/5000 dilution

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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