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Mouse Monoclonal 6X His tag® antibody. Suitable for IP, Flow Cyt, ELISA, Dot, WB, ICC/IF, AP and reacts with Transfected cell lysate, Tag, Transfected cell line samples. Cited in 30 publications.
Alternative names=6 His epitope tag, Glutathione S Transferase, Glutathione S-transferase class-mu 26 kDa isozyme, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, Sj26 antigen, SjGST
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ELISA | Dot | WB | ICC/IF | AP | |
|---|---|---|---|---|---|---|---|
| Tag | Not recommended | Expected | Expected | Expected | Not recommended | Not recommended | Expected |
| Transfected cell line | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Transfected cell lysate | Tested | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Images
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes See Abreview. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag, Transfected cell line | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag, Transfected cell line | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Tag | Dilution info - | Notes - |
AP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal 6X His tag® antibody. Suitable for IP, Flow Cyt, ELISA, Dot, WB, ICC/IF, AP and reacts with Transfected cell lysate, Tag, Transfected cell line samples. Cited in 30 publications.
Alternative names=6 His epitope tag, Glutathione S Transferase, Glutathione S-transferase class-mu 26 kDa isozyme, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, Sj26 antigen, SjGST
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
4D11
- Purification technique
Affinity purification Protein G
- Epitope
This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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