Mouse Monoclonal 6X His tag® antibody. Suitable for IP, Flow Cyt, ELISA, Dot, WB, ICC/IF, AP and reacts with Transfected cell lysate, Tag, Transfected cell line samples. Cited in 29 publications.
Images
![Immunoprecipitation - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--immunoprecipitation-img139881.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-6X His tag® antibody [4D11] (AB5000)")
![Western blot - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--western-blot-img87837.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-6X His tag® antibody [4D11] (AB5000)")
![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--immunocytochemistry-immunofluorescence-img10021.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (AB5000)")
![Western blot - Anti-6X His tag® antibody [4D11] (AB5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--western-blot-img103900.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-6X His tag® antibody [4D11] (AB5000)")
Publications
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- Journal of lipid research 62:1001152021A simple, rapid, and sensitive fluorescence-based method to assess triacylglycerol hydrolase activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSujith Rajan,Hazel C de Guzman,Thomas Palaia,Ira J Goldberg,M Mahmood HussainPubMed 34508728
- The Journal of clinical investigation 131:2021SUMOylation promotes extracellular vesicle-mediated transmission of lncRNA ELNAT1 and lymph node metastasis in bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChanghao Chen,Hanhao Zheng,Yuming Luo,Yao Kong,Mingjie An,Yuting Li,Wang He,Bowen Gao,Yue Zhao,Hao Huang,Jian Huang,Tianxin LinPubMed 33661764
- Frontiers in cell and developmental biology 8:5594862020S100A10 Accelerates Aerobic Glycolysis and Malignant Growth by Activating mTOR-Signaling Pathway in Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Li et. al.PubMed 33324631
- Autophagy 17:1714-17282020PKD2/polycystin-2 induces autophagy by forming a complex with BECN1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Peña-Oyarzun,Marcelo Rodriguez-Peña,Francesca Burgos-Bravo,Angelo Vergara,Catalina Kretschmar,Cristian Sotomayor-Flores,Cesar A Ramirez-Sarmiento,Humbert De Smedt,Montserrat Reyes,William Perez,Vicente A Torres,Eugenia Morselli,Francisco Altamirano,Christian A M Wilson,Joseph A Hill,Sergio Lavandero,Alfredo CriolloPubMed 32543276
- Oncoimmunology 9:17476882020CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuangwei Lv et. al.PubMed 32363119
- eLife 8:2019Aberrant calcium channel splicing drives defects in cortical differentiation in Timothy syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgia Panagiotakos et. al.PubMed 31868578
- Drug testing and analysis 11:1675-16972019Detection of black market follistatin 344.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Reichel,Günter Gmeiner,Mario ThevisPubMed 31758732
- Bio-protocol 9:e34082019Isolation and Imaging of His- and RFP-tagged Amyloid-like Proteins from by TEM and SIM.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmberley D Stephens,Meng Lu,Gabriele S Kaminski SchierlePubMed 33654909
- Heliyon 5:e015202019GlSir2.1 of is a NAD-dependent cytoplasmic deacetylase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdian A Herrera T et. al.PubMed 31025022
- Neoplasia (New York, N.Y.) 21:413-4272019Lysyl Oxidase-Like Protein 2 Promotes Tumor Lymphangiogenesis and Lymph Node Metastasis in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunying Wang et. al.PubMed 30925417
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ELISA | Dot | WB | ICC/IF | AP | |
|---|---|---|---|---|---|---|---|
| Tag | Not recommended | Expected | Expected | Expected | Not recommended | Not recommended | Expected |
| Transfected cell line | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Transfected cell lysate | Tested | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes See Abreview. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag, Transfected cell line | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag, Transfected cell line | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Tag | Dilution info - | Notes - |
AP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
3 products for Alternative Version
Target data
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Recommended products
Mouse Monoclonal 6X His tag® antibody. Suitable for IP, Flow Cyt, ELISA, Dot, WB, ICC/IF, AP and reacts with Transfected cell lysate, Tag, Transfected cell line samples. Cited in 29 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 4D11
- Purification technique
- Affinity purification
- Epitope
- This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
- Biological function summary
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
- Pathways
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
- Associated diseases and disorders
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--immunoprecipitation-img139881.jpg "Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000)")
This image is courtesy of a customer review submitted by Dr Vladimir MilenkovicImmunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000)
ab5000 Immunoprecipitate in human HEK293 whole cell lysate. 25μg of cell lysate incubated with primary antibody (undiluted) and matrix (Protein G) for 16 hours at 4°C. For western blotting an undiluted HRP-Goat anti-rabbit IgG polyclonal was used. Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab
Lane 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)
Lane 2. IP with anti His Ab
Lane 3. Non bound fraction
All lanes: Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000)
![Western blot - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--western-blot-img87837.jpg "Western blot - Anti-6X His tag® antibody [4D11] (ab5000)")
Western blot - Anti-6X His tag® antibody [4D11] (ab5000)
All lanes: Western blot - Anti-6X His tag® antibody [4D11] (ab5000) at 1/1000 dilution
All lanes: 6X His Tagged Protein at 1 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30s
![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--immunocytochemistry-immunofluorescence-img10021.jpg "Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (ab5000)")
This image is courtesy of a customer review submitted by Dr Vladimir MilenkovicImmunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (ab5000)
ab5000 staining Human HEK 293 cells transfected with CACNB3-His by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody (1/250 in 1% goat serum, 0.1% TX100, 1X PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody (1/500) was used as the secondary. DAPI was used for nuclei staining (blue).
![Western blot - Anti-6X His tag® antibody [4D11] (ab5000), expandable thumbnail](https://content.abcam.com/products/images/6x-his-tag-antibody-4d11-ab5000--western-blot-img103900.jpg "Western blot - Anti-6X His tag® antibody [4D11] (ab5000)")
This image is courtesy of a customer review submitted by Dr Vladimir MilenkovicWestern blot - Anti-6X His tag® antibody [4D11] (ab5000)
All lanes: Western blot - Anti-6X His tag® antibody [4D11] (ab5000) at 1/2000 dilution
Lane 1: Lysates prepared from HEK-293 cells
Lane 2: Lysates prepared from HEK-293 cells transfected with Beta3-His (CACNB3)
SecondaryAll lanes: HRP-conjugated goat anit-mouse IgG polyclonal at 1/5000 dilution
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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