AB137839

Anti-6X His tag® antibody

Lab Essentials
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(18 Publications)

Rabbit Polyclonal 6X His tag® antibody. Suitable for IP, WB, ELISA and reacts with Tag samples. Cited in 18 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

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Immunoprecipitation - Anti-6X His tag® antibody (AB137839)

His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.

All lanes:

Immunoprecipitation - Anti-6X His tag® antibody (ab137839)

Predicted band size: 272 kDa

Observed band size: 125 kDa

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ELISA - Anti-6X His tag® antibody (AB137839)

ELISA

Supplier Data

ELISA - Anti-6X His tag® antibody (AB137839)

ab137839 detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by ab137839 at 2.5 µg/ml. Bound Abs were further detected by using a HRP-conjugated goat anti-rabbit IgG with HRP substrates.

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Western blot - Anti-6X His tag® antibody (AB137839)

12% SDS PAGE

All lanes:

Western blot - Anti-6X His tag® antibody (ab137839) at 1/1000 dilution

Lane 1:

Non-transformed E. Coli lysate at 10 µg

Lane 2:

Non-transformed E. Coli lysate, induced at 10 µg

Lane 3:

E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced at 10 µg

Lane 4:

E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced at 10 µg

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

IP, ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/1000 - 1/10000", "ELISA-species-notes": "" } } }

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Product details

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Nature 633:905-913 PubMed39198643

2024

Fibrin drives thromboinflammation and neuropathology in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Kyu Ryu,Zhaoqi Yan,Mauricio Montano,Elif G Sozmen,Karuna Dixit,Rahul K Suryawanshi,Yusuke Matsui,Ekram Helmy,Prashant Kaushal,Sara K Makanani,Thomas J Deerinck,Anke Meyer-Franke,Pamela E Rios Coronado,Troy N Trevino,Min-Gyoung Shin,Reshmi Tognatta,Yixin Liu,Renaud Schuck,Lucas Le,Hisao Miyajima,Andrew S Mendiola,Nikhita Arun,Brandon Guo,Taha Y Taha,Ayushi Agrawal,Eilidh MacDonald,Oliver Aries,Aaron Yan,Olivia Weaver,Mark A Petersen,Rosa Meza Acevedo,Maria Del Pilar S Alzamora,Reuben Thomas,Michela Traglia,Valentina L Kouznetsova,Igor F Tsigelny,Alexander R Pico,Kristy Red-Horse,Mark H Ellisman,Nevan J Krogan,Mehdi Bouhaddou,Melanie Ott,Warner C Greene,Katerina Akassoglou

Oncology reports 49: PubMed37083097

2023

Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijuan Du,Kai Li,Zijun Zhou,Yunlong Huang,Kelei Guo,Hui Zhang,Zhiguo Chen,Xulin Zhao,Li Han,Hua Bian

Journal of cell science 135: PubMed36000409

2022

Conserved Pib2 regions have distinct roles in TORC1 regulation at the vacuole.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla K Troutman,Natalia V Varlakhanova,Bryan A Tornabene,Rajesh Ramachandran,Marijn G J Ford

International journal of molecular sciences 23: PubMed35955841

2022

Regulatory T Cell Depletion Using a CRISPR Fc-Optimized CD25 Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Marit J van Elsas,Johan M S van der Schoot,Alexander Bartels,Kas Steuten,Duco van Dalen,Zacharias Wijfjes,Carl G Figdor,Thorbald van Hall,Sjoerd H van der Burg,Martijn Verdoes,Ferenc A Scheeren

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22318 PubMed35648586

2022

Laminin N-terminus α31 expression during development is lethal and causes widespread tissue-specific defects in a transgenic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Conor J Sugden,Valentina Iorio,Lee D Troughton,Ke Liu,Mychel R P T Morais,Rachel Lennon,George Bou-Gharios,Kevin J Hamill

Science advances 7:eabf7606 PubMed34890220

2021

Neuroserpin and transthyretin are extracellular chaperones that preferentially inhibit amyloid formation.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer West,Sandeep Satapathy,Daniel R Whiten,Megan Kelly,Nicholas J Geraghty,Emma-Jayne Proctor,Pietro Sormanni,Michele Vendruscolo,Joel N Buxbaum,Marie Ranson,Mark R Wilson

Cell death and differentiation 29:14-27 PubMed34262144

2021

CDK15 promotes colorectal cancer progression via phosphorylating PAK4 and regulating β-catenin/ MEK-ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chuntian Huang,Ruijuan Du,Xuechao Jia,Kangdong Liu,Yan Qiao,Qiong Wu,Ning Yao,Lu Yang,Liting Zhou,Xuejiao Liu,Pu Xiang,Mingxia Xin,Yan Wang,Xiaojie Chen,Dong Joon Kim,Zigang Dong,Xiang Li

Science advances 5:eaaw1822 PubMed31489367

2019

Functional diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Johan M S van der Schoot,Felix L Fennemann,Michael Valente,Yusuf Dolen,Iris M Hagemans,Anouk M D Becker,Camille M Le Gall,Duco van Dalen,Alper Cevirgel,Jaco A C van Bruggen,Melanie Engelfriet,Tomislav Caval,Arthur E H Bentlage,Marieke F Fransen,Maaike Nederend,Jeanette H W Leusen,Albert J R Heck,Gestur Vidarsson,Carl G Figdor,Martijn Verdoes,Ferenc A Scheeren

Molecular cell 74:729-741.e7 PubMed30982745

2019

Dual Role of Ribosome-Binding Domain of NAC as a Potent Suppressor of Protein Aggregation and Aging-Related Proteinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Koning Shen,Martin Gamerdinger,Rebecca Chan,Karina Gense,Esther M Martin,Nadine Sachs,Patrick D Knight,Renate Schlömer,Antonio N Calabrese,Katie L Stewart,Lukas Leiendecker,Ankit Baghel,Sheena E Radford,Judith Frydman,Elke Deuerling

Journal of cell science 131: PubMed30097557

2018

Ivy1 is a negative regulator of Gtr-dependent TORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia V Varlakhanova,Bryan A Tornabene,Marijn G J Ford

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-6X His tag® antibody

Immunoprecipitation - Anti-6X His tag® antibody (AB137839)

ELISA - Anti-6X His tag® antibody (AB137839)

Western blot - Anti-6X His tag® antibody (AB137839)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise