AB9107

Anti-6X His tag® antibody

Name: Anti-6X His tag® antibody (ab9107) | Abcam
Brand: Abcam Limited
SKU: AB9107

Be the first to review this product! Submit a review

(2 Publications)

Chicken Polyclonal 6X His tag® antibody. Suitable for ELISA, WB and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Applications

WB, ELISA

applications

Specificity

Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.

style

left-column

Alternative Products

Primary Antibodies

AB5000

Anti-6X His tag® antibody [4D11]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (AB5000)

Primary Antibodies

AB202004

HRP Anti-6X His tag® antibody [4D11]

Western blot - HRP Anti-6X His tag® antibody [4D11] (AB202004)

Primary Antibodies

AB245203

Anti-6X His tag® antibody [JPAR-2] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-6X His tag® antibody [JPAR-2] - BSA and Azide free (AB245203)

Primary Antibodies

AB213204

Anti-6X His tag® antibody [EPR20547] - ChIP Grade

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Primary Antibodies

AB245114

Anti-6X His tag® antibody [JPAR-2]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Flow Cytometry - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Primary Antibodies

AB232492

Anti-6X His tag® antibody [EPR20547] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (AB232492)

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody was an effective at 1:1000 (1 μg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding.</p>" } } }

style

left-column

Product details

HIS-TAG® is a trademark of EMD Biosciences, Inc.

style

left-column

Properties and storage information

Form

Liquid

Purity

IgY fraction

Purification notes

Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information 6X His tag®

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40867552

2025

ENO1 from Disrupts Host Glycolysis and Inflammation by Binding ACTB.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Rui Li,Xiao-Jiao Yu,Jia-Yin Liang,Jin-Liang Sheng,Hui Zhang,Chuang-Fu Chen,Zhong-Chen Ma,Yong Wang

Bio-protocol 10:e3783 PubMed33659438

2020

Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides.

Applications

Unspecified application

Species

Unspecified reactive species

Kalina T Haas,Methieu Rivière,Raymond Wightman,Alexis Peaucelle

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com