Anti-6X His tag® antibody is a rabbit polyclonal antibody that is used to detect 6X His tag® in Western blow, ChIP, ELISA, ICC/IF.
- Antibody specificity verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA)
- Cited in over 160 publications
- Trusted since 2002
Images
Publications
Filter by
- Nature communications 14:79582023Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMomei Zhou,Benjamin Vollmer,Emily Machala,Muyuan Chen,Kay Grünewald,Ann M Arvin,Wah Chiu,Stefan L OliverPubMed 38042814
- Nature communications 14:76542023Regulation of the physiology and virulence of Ralstonia solanacearum by the second messenger 2',3'-cyclic guanosine monophosphate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Li,Wenfang Yin,Junjie Desmond Lin,Yong Zhang,Quan Guo,Gerun Wang,Xiayu Chen,Binbin Cui,Mingfang Wang,Min Chen,Peng Li,Ya-Wen He,Wei Qian,Haibin Luo,Lian-Hui Zhang,Xue-Wei Liu,Shihao Song,Yinyue DengPubMed 37996405
- Philosophical transactions of the Royal Society of London. Series B, Biological sciences 378:202204512023Co-expression of PADI isoforms during progenitor differentiation enables functional diversity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKim Vikhe Patil,Mandy Meijer,Kylie Hin-Man Mak,Wei Yang,Ana Mendanha Falcão,Gonçalo Castelo-Branco,Maria GenanderPubMed 37778375
- Nature communications 14:39162023A PPP-type pseudophosphatase is required for the maintenance of basal complex integrity in Plasmodium falciparum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander A Morano,Rachel M Rudlaff,Jeffrey D DvorinPubMed 37400439
- Cell communication and signaling : CCS 21:1342023Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenchang Xu,Xinqi Liu,Wenjuan Han,Keling Wu,Minglei Zhao,Tingfang Mei,Bizhi Shang,Jinwen Wu,Jingyi Luo,Yuhua Lai,Boyu Yang,Yehong Zhuo,Lin Lu,Yizhi Liu,Xiao-Li Tian,Ling ZhaoPubMed 37316948
- International journal of molecular sciences 24:2023Revisiting Fur Regulon Leads to a Comprehensive Understanding of Iron and Fur Regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaofan Hou,Lin Liu,Xian Ju,Yunzhu Xiao,Bingyu Li,Conghui YouPubMed 37240425
- Cell 186:2425-2437.e212023RNA polymerase drives ribonucleotide excision DNA repair in E. coli.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhitai Hao,Manjunath Gowder,Sergey Proshkin,Binod K Bharati,Vitaly Epshtein,Vladimir Svetlov,Ilya Shamovsky,Evgeny NudlerPubMed 37196657
- Journal of integrative plant biology 65:1937-19492023The F-box protein SHORT PRIMARY ROOT modulates primary root meristem activity by targeting SEUSS-LIKE protein for degradation in rice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNini Ma,Nian Li,Zhongmao Yu,Chunli Chen,Dao-Xiu Zhou,Yu ZhaoPubMed 37013933
- Heliyon 9:e159452023Protective effects of cell permeable Tat-PIM2 protein on oxidative stress induced dopaminergic neuronal cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jea Shin,Won Sik Eum,Gi Soo Youn,Jung Hwan Park,Hyeon Ji Yeo,Eun Ji Yeo,Hyun Jung Kwon,Eun Jeong Sohn,Lee Re Lee,Na Yeon Kim,Su Yeon Kwon,Su Min Kim,Hyo Young Jung,Duk-Soo Kim,Sung-Woo Cho,Oh-Shin Kwon,Dae Won Kim,Soo Young ChoiPubMed 37223703
- Journal of cellular physiology 238:1507-15192023IFI16/Ifi202 released from breast cancer induces secretion of inflammatory cytokines from macrophages and promotes tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGa Young Lim,Sun Wook Cho,Na-Lee Ka,Kyung-Hun Lee,Seock-Ah Im,Seung-Su Kim,Sewon Hwang,Mi-Ock LeePubMed 37062941
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Consider this alternative
Reactivity data
Select an application| ChIP | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Tag | Tested | Expected | Tested | Tested |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 5 µg/mL | Notes ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/500 | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Recommended products
Anti-6X His tag® antibody is a rabbit polyclonal antibody that is used to detect 6X His tag® in Western blow, ChIP, ELISA, ICC/IF.
- Antibody specificity verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA)
- Cited in over 160 publications
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Immunogen
- Specificity
ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:25,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using HRP-conjugated Goat anti-Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-6X His tag® antibody (ab9108) is a rabbit polyclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, WB in human, mouse, rat samples.
Anti-6X His tag® antibody (ab9108) has been cited over 165 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-6X His tag® antibody (ab9108) has high sensitivity and specificity.
Anti-6X His tag® antibody (ab9108) has 25 independent reviews from customers.
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-6X His tag® antibody (ab9108) has been validated in ChIP, ELISA, ICC/IF, WB 25 165
Anti-6X His tag® antibody (ab9108) specifically detects 6X His tag® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in 100 µg selling sizes.
This antibody recognizes both C-terminal and N-terminal tags.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Or search our wide range of secondary antibodies for use with your experiment.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
- Biological function summary
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
- Pathways
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
- Associated diseases and disorders
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
This image is courtesy of Vladimir MilenkovicImmunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody (ab9108)
ab9108 staining CACNB3 with His tag expressed in HEK293 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% goat serum, 0.1% TX100 and PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/750).
ChIP - Anti-6X His tag® antibody (ab9108)
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
This image is courtesy of an anonymous customer reviewWestern blot - Anti-6X His tag® antibody (ab9108)
All lanes: Western blot - Anti-6X His tag® antibody (ab9108) at 1/4000 dilution
Lane 1: HeLa whole cell lysate - transfected with empty vector at 10 µg
Lane 2: HeLa whole cell lysate - transfected with His-tagged gene at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 35 kDa
Exposure time: 30s
Western blot - Anti-6X His tag® antibody (ab9108)
Use of ab9108 in western blotting at 1:1000 against Epitope Tag Positive Control lysate (ab4524).
Lane 1: 2 µl lysate
Lane 2: 1 µl lysate
Lane 3: 0.5 µl lysate
Lane 4: 0.25 µl lysate Use of ab9108 in western blotting at 1:1000 against E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (E. coli Positive Control (Escherichia coli ) Whole Cell Lysate ab5395).
Lane 1: 2 µl lysate
Lane 2: 1 µl lysate
Lane 3: 0.5 µl lysate
Lane 4: 0.25 µl lysateAll lanes: Western blot - Anti-6X His tag® antibody (ab9108)
Western blot - Anti-6X His tag® antibody (ab9108)
Lanes 1 - 5: Green – Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free ab282749 at 1 in 500 dilution, observed from 120 – 180 kDa
Lanes 6 - 8: Red – Rabbit polyclonal to 6X His tag® (ab9108) at 1 in 1000 dilution, observed from 120 – 160 kDa
Lanes 9 & 10: Black – Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence) at 1 μg/mL, observed at 180 kDaAnti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free ab282749 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.
Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T).
Lanes 1 - 5: Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free ab282749) at 1/500 dilution
Lanes 6 - 8: Western blot - Anti-6X His tag® antibody (ab9108) at 1/1000 dilution
Lanes 9 - 10: Goat polyclonal to DDDDK tag at 1 µg/mL
Lanes 1 and 6: Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.5 µg
Lanes 2 and 7: SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg
Lanes 3 and 8: SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg
Lanes 4 and 9: SARS-CoV-2 3xFlag Spike Protein Transfected Expi whole cell lysate at 20 µg
Lanes 5 and 10: SARS-CoV 3xFlag Spike Protein Transfected Expi whole cell lysate at 20 µg
SecondaryLanes 1 - 5: Western blot - Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216778) at 1/20000 dilution
Lanes 6 - 8: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution
Lanes 9 - 10: Western blot - Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed ab216775) at 1/20000 dilution
Observed band size: 120-180 kDa, 120-160 kDa, 180 kDa
Western blot - Anti-6X His tag® antibody (ab9108)
Lane 1 and 4: Molecular weight Standards.
Primary and secondary antibody incubation at RT for 1 hour.All lanes: Western blot - Anti-6X His tag® antibody (ab9108) at 1 µg/mL
Lane 1: Purified 12-Tag at 2 µg
Lane 2: Purified 12-Tag at 0.2 µg
Lane 3: The cell lysates of CHO-12 Tags at 1 µg
Lane 4: The cell lysates of CHO at 2 µg
SecondaryAll lanes: Goat anti-rabbit, HRP conjugated at 0.2 µg/mL
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody (ab9108)
Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 in CHO cells transfected with 12 tags constructs (CHO-12 tags) labelling 6xHIS with ab9108 at 1/1000 dilution (1µg/mL), followed by Donkey Anti-rabbit IgG (H&L), Alexa Fluor® 594 antibody at 1/500 dilution (4 µg/mL) (Red). Nucleus was counterstained with DAPI (Blue). Bottom panel images showed Parallel staining in parental CHO cell line.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com