Anti-6X His tag® antibody [AD1.1.10]

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human GDAP1L1.

In Western blot, Anti-His tag antibody - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-GDAP1 antibody [EPR29581-48] (<a href='/en-us/products/primary-antibodies/gdap1-antibody-epr29581-48-ab323844'>ab323844</a>) at 1/1000 dilution

Lane 1:

Purified 293T cells transfected with a human GDAP1 expression vector containing a His-tag at 20 µg

Lane 2:

Purified 293T cells transfected with a human GDAP1L1 expression vector containing a His-tag at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa

false

Exposure time: 180s

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with His and Myc tagged cas9 construct cells labelling 6X His tag with ab15149 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution(Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with His and Myc tagged mouse cas9 construct. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).

ab32072 Anti-c-Myc rabbit monoclonal antibody was used to counterstain Myc at 1/500 followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red).

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human SV2B or SV2A by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SV2C antibody [EPR30548-547] (<a href='/en-us/products/primary-antibodies/sv2c-antibody-epr30548-547-ab324233'>ab324233</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human SV2C expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human SV2B expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 4:

293T cells transfected with a human SV2A expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 82 kDa,36 kDa,77-82 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human THRA by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) & Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/200000 and 1 : 1000 dilutions respectively.

All lanes:

Western blot - Anti-Thyroid Hormone Receptor beta antibody [EPR28919-182] (<a href='/en-us/products/primary-antibodies/thyroid-hormone-receptor-beta-antibody-epr28919-182-ab324308'>ab324308</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human THRB expression vector containi a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human THRA expression vector containi a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 52 kDa,54 kDa,36 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human MTHFD1L by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-MTHFD1 antibody [EPR29810-508] (<a href='/en-us/products/primary-antibodies/mthfd1-antibody-epr29810-508-ab324379'>ab324379</a>) at 1/1000 dilution

Lane 1:

No-tagged human MTHFD1 fragment at 10 ng

Lane 2:

His-tagged human MTHFD1L fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,30 kDa

false

Exposure time: 15s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of JAK1 using ab324683 at 1 : 1000 (0.506 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human JAK1 recombinant fragment
Lane2 : His-tagged human JAK2 recombinant fragment
Lane3 : His-tagged human JAK3 recombinant fragment
Lane4 : His-tagged human TYK2 recombinant fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human JAK2, JAK3 or TYK2 by dot blot.

#In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-JAK1 antibody [EPR24649-58] (<a href='/en-us/products/primary-antibodies/jak1-antibody-epr24649-58-ab324683'>ab324683</a>) at 1/1000 dilution

Lane 1:

His-tagged human JAK1 recombinant fragment

Lane 2:

His-tagged human JAK2 recombinant fragment

Lane 3:

His-tagged human JAK3 recombinant fragment

Lane 4:

His-tagged human TYK2 recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of N-terminal LPHN2 using ab324484 at 1 : 1000 (0.495 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human LPHN2 recombinant fragment
Lane2 : His-tagged human LPHN1 recombinant fragment
Lane3 : His-tagged human LPHN3 recombinant fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human LPHN1 or LPHN3.

#In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (<a href='/en-us/products/primary-antibodies/n-terminal-lphn2-antibody-epr29557-9-ab324484'>ab324484</a>) at 1/1000 dilution

Lane 1:

His-tagged human LPHN2 recombinant fragment

Lane 2:

His-tagged human LPHN1 recombinant fragment

Lane 3:

His-tagged human LPHN3 recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of FURIN using ab324440 at 1 : 1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : No-tagged human PCSK3 recombinant protein
Lane2 : His-tagged human PCSK1 recombinant protein
Lane3 : His-tagged human PCSK4 recombinant protein
Lane4 : His-tagged human PCSK5 recombinant protein
Lane5 : His-tagged human PCSK6 recombinant protein

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PCSK1, PCSK4, PCSK5, and PCSK6.

In Dot blot, anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-FURIN antibody [RM2083] (<a href='/en-us/products/primary-antibodies/furin-antibody-rm2083-ab324440'>ab324440</a>) at 1/1000 dilution

Lane 1:

No-tagged human PCSK3 recombinant protein with NFDM/TBST

Lane 2:

His-tagged human PCSK1 recombinant protein with NFDM/TBST

Lane 3:

His-tagged human PCSK4 recombinant protein with NFDM/TBST

Lane 4:

His-tagged human PCSK5 recombinant protein with NFDM/TBST

Lane 5:

His-tagged human PCSK6 recombinant protein with NFDM/TBST

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• I-ELISA

Lab

Indirect ELISA - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Indirect ELISA analysis of ab15149 at 5000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution.

Antigen : 6X His tag®.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse ZIC1, ZIC2, ZIC4 and ZIC5.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody AD1.1.10 staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ZIC3 antibody [EPR30592-522] (<a href='/en-us/products/primary-antibodies/zic3-antibody-epr30592-522-ab325172'>ab325172</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse ZIC3 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse ZIC1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse ZIC2 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse ZIC4 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse ZIC5 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed mouse Angiopoietin 2, Angiopoietin 3 and Angiopoietin 4 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Angiogenin antibody [RM2092] (<a href='/en-us/products/primary-antibodies/angiogenin-antibody-rm2092-ab325241'>ab325241</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Angiogenin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse Angiopoietin 2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse Angiopoietin 3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse Angiopoietin 4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 16 kDa,36 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

In Western blot, Anti-6X His tag® antibody [AD1.1.10] total protein control and secondary antibody used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab32560 does not react with other 14-3-3 protein families.

All lanes:

Western blot - Anti-14-3-3 alpha + beta antibody [Y62] (<a href='/en-us/products/primary-antibodies/14-3-3-alpha-beta-antibody-y62-ab32560'>ab32560</a>) at 1/1000 dilution

Lane 1:

Human 14-3-3 beta (YWHAB) Recombinant Protein with a his tag

Lane 2:

Human 14-3 3 gamma (YWHAG) Recombinant Protein with a his tag

Lane 3:

Human 14-3-3 epsilon (YWHAE) Recombinant Protein with a his tag

Lane 4:

Human 14-3-3 zeta (YWHAZ) Recombinant Protein with a his tag

Lane 5:

Human 14 3 3 eta (YWHAH) Recombinant Protein with a his tag

Lane 6:

Human 14-3-3 theta (YWHAQ) Recombinant Protein with a his tag

Lane 7:

Human 14-3-3 sigma (SFN) Recombinant Protein with a his tag

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa

false

Exposure time: 42s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody cross-reacts with overexpressed mouse PRSS2 and PRSS3 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Trypsin antibody [EPR29805-7] (<a href='/en-us/products/primary-antibodies/trypsin-antibody-epr29805-7-ab325233'>ab325233</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with mouse PRSS1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with mouse PRSS2 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T transfected with mouse PRSS3 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,28 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human CAPN1, CAPN3, CAPN8, CAPN9 or CAPN11 by western blot.

In Western blot, Anti-His tag antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Calpain 2 antibody [EPR29833-94] (<a href='/en-us/products/primary-antibodies/calpain-2-antibody-epr29833-94-ab325140'>ab325140</a>) at 1/1000 dilution

Lane 1:

His-tagged human CAPN2 fragment at 10 ng

Lane 2:

His-tagged human CAPN1 fragment at 10 ng

Lane 3:

His-tagged human CAPN3 fragment at 100 ng

Lane 4:

His-tagged human CAPN8 fragment at 10 ng

Lane 5:

His-tagged human CAPN9 fragment at 10 ng

Lane 6:

His-tagged human CAPN11 fragment at 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 34 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Beta-synuclein.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Alpha-synuclein (N-term) antibody [EPR29522-6] (<a href='/en-us/products/primary-antibodies/alpha-synuclein-n-term-antibody-epr29522-6-ab324977'>ab324977</a>) at 1/1000 dilution

Lane 1:

His-tagged human Alpha-synuclein recombinant protein at 10 ng

Lane 2:

His-tagged human Beta-synuclein recombinant protein at 10 ng

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 18 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at a 1/200000 dilution. Anti-6X His tag® antibody [AD1.1.10](ab15149) used for total protein control.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Pneumolysin antibody [PLY-4] (<a href='/en-us/products/primary-antibodies/pneumolysin-antibody-ply-4-ab71810'>ab71810</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 40 µg

Lane 2:

293T transfected with Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) expression vector containing a myc-His-tag® whole cell lysate at 40 µg

Lane 3:

293T transfected with Streptococcus pyogenes serotype M3 (strain SSI-1) expression vector containing a myc-His-tag® whole cell lysate at 40 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 53 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

In Western blot, Anti-Anti-6X His tag® antibody [AD1.1.10] loading control staining at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human light chain Factor XII.

All lanes:

Western blot - Anti-Factor XII antibody [EPR22292-127] (<a href='/en-us/products/primary-antibodies/factor-xii-antibody-epr22292-127-ab242123'>ab242123</a>) at 1/1000 dilution

Lane 1:

His-tagged heavy chain of human Factor XII recombinant protein at 10 ng

Lane 2:

Lif/His-tagged light chain of human Factor XII recombinant protein at 10 ng

Lane 3:

No-tagged human Factor XII recombinant protein without His tag at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 65 kDa,50-70 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag^® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ESM1 antibody [RM1338] (<a href='/en-us/products/primary-antibodies/esm1-antibody-rm1338-ab323845'>ab323845</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a human ESM1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse TAP2.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-His tag antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-TAP1 antibody [EPR30551-512] (<a href='/en-us/products/primary-antibodies/tap1-antibody-epr30551-512-ab324718'>ab324718</a>) at 1/1000 dilution

Lane 1:

Untagged mouse TAP1 fragment at 10 ng

Lane 2:

His-tagged mouse TAP2 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa,34 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PAX7.

In Western blot, Anti-His tag® antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-PAX3 antibody [EPR29741-157] (<a href='/en-us/products/primary-antibodies/pax3-antibody-epr29741-157-ab324432'>ab324432</a>) at 1/1000 dilution

Lane 1:

Untagged human PAX3 fragment at 10 ng

Lane 2:

His-tagged human PAX7 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 26 kDa,24 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human PYCR1 or PYCR3 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-PYCR2 antibody [EPR28825-54] (<a href='/en-us/products/primary-antibodies/pycr2-antibody-epr28825-54-ab324352'>ab324352</a>) at 1/1000 dilution

Lane 1:

His-tagged human PYCR1 recombinant protein at 10 ng

Lane 2:

His-tagged human PYCR2 recombinant protein at 10 ng

Lane 3:

His-tagged human PYCR3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa,33 kDa,29 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : QD20250827IMG37.

This antibody does not cross-react with recombinant human FCN2 or FCN1 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10]- (ab15149) staining at 1/1000 dilution.

Exposure time : Lane 1 : 3 seconds; Lanes 2-4 : 10 seconds.

All lanes:

Western blot - Anti-Ficolin-3 antibody [EPR30745-69] (<a href='/en-us/products/primary-antibodies/ficolin-3-antibody-epr30745-69-ab324673'>ab324673</a>) at 1/1000 dilution

Lane 1:

Human FCN3 recombinant protein (no-tag) at 10 ng

Lane 2:

His-tagged human FCN2 recombinant protein at 10 ng

Lane 3:

His-tagged human FCN1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa,26 kDa

false

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-His tag® antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Dengue NS3 antibody [EPR29361-53] (<a href='/en-us/products/primary-antibodies/dengue-ns3-antibody-epr29361-53-ab324565'>ab324565</a>) at 1/5000 dilution

Lane 1:

293T (human epithelial cell line from embryonic kidney) cells transfected with an empty vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Virus Dengue type 2 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a Virus Dengue type 4 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 4:

293T transfected with a Virus Dengue type 3 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 5:

293T transfected with a Virus Dengue type 1 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human ETV1 and ETV5.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 37002241).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Pea3 antibody [EPR30314-30] (<a href='/en-us/products/primary-antibodies/pea3-antibody-epr30314-30-ab323843'>ab323843</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ETV4 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human ETV1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human ETV5 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 54-61 kDa,36 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human Cytochrome P450 2C19 or Cytochrome P450 2C18 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Cytochrome P450 2E1 antibody [EPR28698-543] (<a href='/en-us/products/primary-antibodies/cytochrome-p450-2e1-antibody-epr28698-543-ab324160'>ab324160</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a human Cytochrome P450 2E1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with a human Cytochrome P450 2C19 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T transfected with a human Cytochrome P450 2C18 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 57 kDa,36 kDa,56 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-GPA33 antibody [EPR30348-518] (<a href='/en-us/products/primary-antibodies/gpa33-antibody-epr30348-518-ab324562'>ab324562</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a mouse GPA33 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 43 kDa,36 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 1/5000 dilution

Lane 1:

His-tagged mouse Cathepsin D recombinant protein (aa65-310; 27kDa)

Lane 2:

His-tagged mouse CDKN2A/p16INK4a recombinant protein (aa1-168;18kDa)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 27 kDa,18 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human RG9MTD1 and RG9MTD2

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (<a href='/en-us/products/primary-antibodies/rg9mtd3-antibody-epr28590-15-ab324235'>ab324235</a>) at 1/1000 dilution

Lane 1:

His-tagged human RG9MTD2 recombinant protein at 10 ng

Lane 2:

His-tagged human RG9MTD3 recombinant protein at 10 ng

Lane 3:

His-tagged human RG9MTD1 recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 44 kDa,30 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with firefly luciferase expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

HEK-293T transfected with human histone H3.1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 61 kDa,18 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Nipah Virus Glycoprotein F antibody [CG11] (<a href='/en-us/products/primary-antibodies/nipah-virus-glycoprotein-f-antibody-cg11-ab325231'>ab325231</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with Nipah virus Fusion glycoprotein F0 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with Hendra virus (isolate Horse/Autralia/Hendra/1994) Fusion glycoprotein F0 expression vector containing a myc-His-tag® whole cell lysate at 2 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1 : 1000 dilution.

All lanes:

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (<a href='/en-us/products/primary-antibodies/lassa-fever-virus-gp1-antibody-ha6-ab325160'>ab325160</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with Lassa virus (strain GA391) Glycoprotein G1(GP1) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with Lassa virus (strain GA391) Glycoprotein G2(GP2) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T transfected with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) Glycoprotein G1(GP1) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 5:

293T transfected with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) Glycoprotein G2(GP2) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 25 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (<a href='/en-us/products/primary-antibodies/rift-valley-fever-virus-nucleoprotein-antibody-aa11-ab325011'>ab325011</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with Rift valley fever virus (RVFV) Nucleoprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 1 µg

Lane 2:

293T cells transfected with Rift valley fever virus (RVFV) Envelopment polyprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with Rift valley fever virus (RVFV) RNA-directed RNA polymerase L (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with Rift valley fever virus (RVFV) Non-structural protein S (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 5 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 28-243 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human UBQLN2 or UBQLN3 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

In Western blot, Anti-LIF antibody [EPR21713-25] - (ab244795) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ubiquilin/UBQLN1 antibody [EPR29585-56] (<a href='/en-us/products/primary-antibodies/ubiquilin-ubqln1-antibody-epr29585-56-ab325320'>ab325320</a>) at 1/1000 dilution

Lane 1:

His-tagged human UBQLN1 recombinant protein at 10 ng

Lane 2:

His-tagged human UBQLN2 recombinant protein at 10 ng

Lane 3:

LIF-tagged human UBQLN3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CST4 and CST1.

In Western blot, Anti-His tag® antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (<a href='/en-us/products/primary-antibodies/cystatin-sa-cst2-antibody-epr25934-14-ab325126'>ab325126</a>) at 1/1000 dilution

Lane 1:

Untagged human Cystatin SA/CST2 recombinant protein at 10 ng

Lane 2:

His-tagged human CST4 recombinant protein at 10 ng

Lane 3:

His-tagged human CST1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 14 kDa,15-16 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human VPS4B by western blot.

In Western blot, Anti-His tag antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-VPS4a antibody [EPR30587-510] (<a href='/en-us/products/primary-antibodies/vps4a-antibody-epr30587-510-ab325430'>ab325430</a>) at 1/1000 dilution

Lane 1:

His-tagged human VPS4A recombinant protein at 10 ng

Lane 2:

His-tagged human VPS4B recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,34 kDa

false

Exposure time: 37s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

This antibody does not cross-react with human KIF13B.

#In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-KIF13A antibody [EPR29130-81] (<a href='/en-us/products/primary-antibodies/kif13a-antibody-epr29130-81-ab323873'>ab323873</a>) at 1/1000 dilution

Lane 1:

Purified 293T cells transfected with a human KIF13A expression vector containing a His-tag, whole cell lysate

Lane 2:

Purified 293T cells transfected with a human KIF13B expression vector containing a His-tag, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of Nrf2 using ab325240 at 1 : 1000 (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T cells transfected with a mouse Nrf2 expression vector containing a myc-His-tag®, whole cell lysate
Lane2 : 293T cells transfected with a mouse NF2L1 expression vector containing a myc-His-tag®, whole cell lysate
Lane3 : 293T cells transfected with a mouse NF2L3 expression vector containing a myc-His-tag®, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse NF2L1 and NF2L3

#In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse Nrf2 expression vector containing a myc-His-tag®, whole cell lysate

Lane 2:

293T cells transfected with a mouse NF2L1 expression vector containing a myc-His-tag®, whole cell lysate

Lane 3:

293T cells transfected with a mouse NF2L3 expression vector containing a myc-His-tag®, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human EMP2.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-His tag® antibody - (ab15149) (1 : 1000)(20-50KDa);Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-EMP-1 antibody [EPR30304-517] (ab325387) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human EMP1 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human EMP2 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 20-50 kDa,36 kDa

false

Exposure time: 1s