AB49936

Anti-6X His tag® antibody [HIS-1]

Lab Essentials
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Mouse Monoclonal 6X His tag® antibody. Suitable for ELISA, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Tag samples. Cited in 5 publications.

HRP

HRP Anti-polyHistidine antibody [HIS-1]
Alkaline Phosphatase

Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIS-1

Isotype

IgG2a

Carrier free

Applications

ELISA, ICC/IF, Dot, WB, Flow Cyt (Intra)

applications

Specificity

ab49939 preferentially reacts to recombinant proteins containing the 6X His tag® fused to amino terminus of fusion proteins.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified from Tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information 6X His tag®

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biofilm 8:100215 PubMed39148892

2024

quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18).

Applications

Unspecified application

Species

Unspecified reactive species

Yael Schlichter Kadosh,Subramani Muthuraman,Khairun Nisaa,Anat Ben-Zvi,Danit Lisa Karsagi Byron,Marilou Shagan,Alexander Brandis,Tevie Mehlman,Jacob Gopas,Rajendran Saravana Kumar,Ariel Kushmaro

iScience 27:108798 PubMed38261928

2024

A novel macrolide-Del-1 axis to regenerate bone in old age.

Applications

Unspecified application

Species

Unspecified reactive species

Kridtapat Sirisereephap,Hikaru Tamura,Jong-Hyung Lim,Meircurius Dwi Condro Surboyo,Toshihito Isono,Takumi Hiyoshi,Andrea L Rosenkranz,Yurie Sato-Yamada,Hisanori Domon,Akari Ikeda,Tomoyasu Hirose,Toshiaki Sunazuka,Nagako Yoshiba,Hiroyuki Okada,Yutaka Terao,Takeyasu Maeda,Koichi Tabeta,Triantafyllos Chavakis,George Hajishengallis,Tomoki Maekawa

Journal of integrative plant biology 64:1706-1723 PubMed35848532

2022

SICKLE represses photomorphogenic development of Arabidopsis seedlings via HY5- and PIF4-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Haojie Li,Hongmei Lian,Pengyu Song,Yulong Wang,Jie Duan,Zhaoqing Song,Yan Cao,Dongqing Xu,Jigang Li,Huiyong Zhang

Cell reports 25:3750-3758.e4 PubMed30590046

2018

A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth R Allen,Stefanie A Krumm,Jayna Raghwani,Steinar Halldorsson,Angela Elliott,Victoria A Graham,Elina Koudriakova,Karl Harlos,Daniel Wright,George M Warimwe,Benjamin Brennan,Juha T Huiskonen,Stuart D Dowall,Richard M Elliott,Oliver G Pybus,Dennis R Burton,Roger Hewson,Katie J Doores,Thomas A Bowden

Journal of bacteriology 198:2419-30 PubMed27381918

2016

The ChrSA and HrrSA Two-Component Systems Are Required for Transcriptional Regulation of the hemA Promoter in Corynebacterium diphtheriae.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan M Burgos,Michael P Schmitt

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-6X His tag® antibody [HIS-1]

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise