AB18184

Anti-6X His tag® antibody [HIS.H8]

Name: Anti-6X His tag® antibody [HIS.H8] (ab18184) | Abcam
Brand: Abcam Limited
SKU: AB18184
Price: 400 USD
Availability: InStock
Rating: 5 (32 reviews)

Lab Essentials
What is this?

(32 Reviews)

(485 Publications)

Anti-6X His tag® antibody [HIS.H8] (ab18184) is a mouse monoclonal antibody detecting 6X His tag® in Western Blot, IP, ICC/IF, ELISA, Dot Blot.

- Over 350 publications
- Trusted since 2005

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

13 Images

AbReview47067****

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

All lanes:

Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution

Lane 1:

6x His-tagged protein expressed in BL21 (pET15b) at 1 µg

Lane 2:

6x His-tagged protein expressed in BL21 (pET15b) at 0.1 µg

Secondary

All lanes:

IRDye® 800CW goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution

false

Exposure time: 5min

This image is courtesy of an Abreview submitted by Rachel Loh

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (AB18184)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (AB18184)

LEFT : untransfected control; RIGHT : anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).

AbReview33604****

Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.

Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa)
Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 μL RRL in 300 μL buffer.
Lanes 6-9 : Purified PDCD5 from E. coli with ~60-70 ng protein.

The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa).

Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.

All lanes:

Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)

false

This image is courtesy of an Abreview submitted by Mr Chris Tracy

ICC/IF

AbReview17028****

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (AB18184)

ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).

This image is courtesy of an anonymous abreview.

AbReview13007****

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Blocked with 5% milk for 30 minutes at 25°C.

Incubated with the primary antibody for 16 hours at 4°C in 1X PBS/Tween, 5% milk.

All lanes:

Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution

Lane 1:

ARPE-19 whole cell lysate - non-transfected at 20 µg

Lane 2:

ARPE-19 whole cell lysate - transfected with rRab27a-His at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution

true

Exposure time: 5min

This image is courtesy of an Abreview submitted by Vladimir Milenkovic

Supplier Data

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

ab286909 was shown to bind specifically to SARS-CoV-2 nsp13 protein in western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Blocking buffer : 5% milk in TBS-0.1% Tween^® 20 (TBS-T)

Lanes 1 - 4:

Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution

Lanes 5 - 8:

Western blot - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] (<a href='/en-us/products/primary-antibodies/sars-cov-2-nsp13-antibody-epr24838-18-ab286909'>ab286909</a>) at 1/1000 dilution

Lanes 1 and 5:

Recombinant SARS-CoV-2 nsp13 protein (His-tagged) at 0.5 µg

Lanes 2 and 6:

Recombinant SARS-CoV-2 nsp13 protein (His-tagged) at 0.2 µg

Lanes 3 and 7:

Recombinant SARS-CoV2 nsp1 protein (His tagged) at 0.5 µg

Lanes 4 and 8:

Recombinant SARS-CoV2 nsp1 protein (His tagged) at 0.2 µg

Secondary

Lanes 1 - 4:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lanes 5 - 8:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

false

Lab

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Lane 1 : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (ab273068) at 0.2 ug

Lane 2 : Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 ug

False colour image of Western blot : Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 Chicken IgY (Chimeric) (ab323001) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, ab323001 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-antibody-epr24852-116-chicken-igy-chimeric-ab323001'>ab323001</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a>) at 0.2 µg

Lane 2:

Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/10000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 135 kDa

false

Exposure time: 4min

Lab

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Lane 1 : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (ab273068) at 0.2 ug

Lane 2 : SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 ug

Performed under reducing conditions.

Observed band size : 120-160 kDa.

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Chicken IgY (Chimeric) (ab322999) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, ab322999 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T).

Exposure time : 2 min.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-antibody-8b12-c2-chicken-igy-chimeric-ab322999'>ab322999</a>) at 1/500 dilution

Lane 1:

Lane 2:

SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/10000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 120-160 kDa

false

Exposure time: 2min

Lab

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Lane 1 : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (ab273068) at 0.2 ug

Lane 2 : Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 ug

Performed under reducing conditions.

Observed band size : 135 kDa.

False colour image of Western blot : Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - Human IgG1 (Chimeric), ab323000 staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, ab323000 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T).

Exposure time : 30 Sec.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-antibody-epr24852-116-human-igg1-chimeric-ab323000'>ab323000</a>) at 1/500 dilution

Lane 1:

Lane 2:

Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 135 kDa

false

Exposure time: 30s

Lab

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot : Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] ab180943 staining at 1/1000 dilution, shown in green; Mouse anti-6x HisTag (ab18184) loading control staining at 1/20,000 dilution, shown in magenta.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution. For DDDDK-tag detection, HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) was used at 1/1000 dilution and developed using the ECL technique.

Exposure time for DDDDK tag : 0.1s.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] (<a href='/en-us/products/primary-antibodies/nse-antibody-epr12483-neuronal-marker-ab180943'>ab180943</a>) at 1/1000 dilution

Lane 1:

ENO1 Recombinant Protein (His Tag) at 0.2 µg

Lane 2:

ENO2 Recombinant Protein (His Tag) at 0.2 µg

Lane 3:

ENO3 Recombinant Protein (His Tag) at 0.2 µg

Lane 4:

ENO1 Recombinant Protein (DDDDK Tag) at 0.2 µg

Lane 5:

ENO2 Recombinant Protein (DDDDK Tag) at 0.2 µg

Lane 6:

ENO3 Recombinant Protein (DDDDK Tag) at 0.2 µg

Secondary

Lanes 1 - 6:

Goat anti-Mouse 680RD at 1/20000 dilution

Lanes 1 - 6:

Goat anti-Rabbit 800CW at 1/20000 dilution

Observed band size: 50 kDa

true

Lab

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Human IgG1 (Chimeric) (ab322272) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/200 dilution, shown in red. In Western blot, ab322272 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-8b12-c2-human-igg1-chimeric-ab322272'>ab322272</a>) at 1/500 dilution

Lane 1:

Lane 2:

SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

Lane 3:

SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Lanes 1 - 3:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 120-160 kDa

false

Exposure time: 5s

Lab

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot : Anti-ENO1 + ENO2 + ENO3 antibody ab53025 staining at 1/1000 dilution, shown in green; Mouse anti-6x HisTag (ab18184) loading control staining at 1/20,000 dilution, shown in magenta.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody (<a href='/en-us/products/primary-antibodies/nse-antibody-neuronal-marker-ab53025'>ab53025</a>) at 1/1000 dilution

Lane 1:

ENO1 Recombinant Protein (His Tag) at 0.2 µg

Lane 2:

ENO2 Recombinant Protein (His Tag) at 0.2 µg

Lane 3:

ENO3 Recombinant Protein (His Tag) at 0.2 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Lab

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

In Western blot, ab286912 was shown to bind specifically to NSP14. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

All lanes:

Western blot - Anti-SARS-CoV-2 nsp14 antibody [EPR24839-87] (<a href='/en-us/products/primary-antibodies/sars-cov-2-nsp14-antibody-epr24839-87-ab286912'>ab286912</a>) at 1/1000 dilution

Lane 1:

Recombinant SARS-CoV-2 NSP14 protein - Active (His-Tagged) at 0.5 µg

Lane 3:

Recombinant SARS-CoV-2 NSP14 (His-tagged) at 0.5 µg

Lane 5:

SARS-CoV-2 NSP1 protein (His-tagged) at 0.5 µg

Lane 7:

Recombinant SARS-COV-2 NSP2 protein (His-tagged) at 0.5 µg

Lane 9:

Recombinant SARS-CoV-2 NSP9 Protein (His-tagged) at 0.5 µg

Secondary

Lanes 1 - 9:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 9:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 64 kDa

false

Biotin

Biotin Anti-6X His tag® antibody [HIS.H8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIS.H8

Isotype

IgG2b

Carrier free

Applications

WB, ELISA, ICC/IF, Dot, IP

applications

Specificity

Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

ab18184 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" } } }

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Product details

Anti-6X His tag^® antibody [HIS.H8] (ab18184) is a mouse monoclonal antibody and is validated for use in Dot, ELISA, ICC/IF, IP and WB.

Anti-6X His tag^® antibody [HIS.H8] (ab18184) was first used in a scientific publication in 2004 and has been cited over 356 times in peer reviewed journals. It's performance in Western blot in human and mouse samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-6X His tag^® antibody [HIS.H8] (ab18184) has high sensitivity and specificity.

Anti-6X His tag^® antibody [HIS.H8] (ab18184) has 31 independent reviews from customers.

6X His tag^® antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-6X His tag^® antibody [HIS.H8] (ab18184) has been validated in Dot, ELISA, ICC/IF, IP and WB.

Anti-6X His tag^® antibody [HIS.H8] (ab18184) specifically detects 6X His tag^® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in 100 ug selling sizes.

Antibody clone HIS.H8 is also available pre-conjugated to a variety of labels for your convenience - Biotin (ab173828).

Top cited 6X His tag antibody in the market with >450 citations. The 6X His tag, also known as a polyhistidine tag, is widely used for the purification of His-tagged proteins. Utilizing Ni-NTA chromatography, this tag facilitates efficient protein purification, ensuring high yield and purity. It is also highly relevant for detecting 6X His tags in multiple proteomic applications such as western blotting (WB), Immunoprecipitation (IP) and immunocytochemistry/immunofluorescence (ICC/IF) applications.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS by Protein A affinity chromatography

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (485)

Recent publications for all applications. Explore the full list and refine your search

Veterinary world 18:2194-2205 PubMed41064820

2025

Multivalent display of VP28 on chimeric virus-like particles enhances binding to shrimp target tissues: A novel antiviral strategy against white spot syndrome virus.

Applications

Unspecified application

Species

Unspecified reactive species

Somkid Jaranathummakul,Pitchanee Jariyapong,Orawan Thongsum,Supawich Boonkua,Charoonroj Chotwiwatthanakun,Monsicha Somrit,Somluk Asuvapongpatana,Attaboon Wathammawut,Wattana Weerachatyanukul

BMC biology 23:283 PubMed41024082

2025

Design and development of a bispecific antibody targeting BAFF and IL-17 for systemic lupus erythematosus treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Xin,Jingming Zhou,Yumei Chen,Yankai Liu,Hongliang Liu,Chao Liang,Xifang Zhu,Yanhua Qi,Gaiping Zhang,Aiping Wang

Viruses 17: PubMed41012691

2025

Rapid Visual Detection of Senecavirus A Based on RPA-CRISPR/Cas12a System with Canonical or Suboptimal PAM.

Applications

Unspecified application

Species

Unspecified reactive species

Xinrui Zhao,Genghong Jiang,Qinyi Ruan,Yunjie Qu,Xiaoyu Yang,Yongyan Shi,Dedong Wang,Jianwei Zhou,Jue Liu,Lei Hou

Scientific reports 15:29716 PubMed40804095

2025

Methylation-induced suppression of BEX1 activates AKT/ERK/STAT3 signaling pathways regulating cell cycle and apoptosis in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Zhi Xiao,Shi Feng,Zhen He,Rui Mao,Kai Qian,Zhi-Ying Zhou,Huabao Cai,Mengyu Zhao,Cunzhi Wang,Tianhang Yu,Zi-Yu Zhao,Jie Li

Nature communications 16:7458 PubMed40796804

2025

Epigenetic control of topoisomerase 1 activity presents a cancer vulnerability.

Applications

Unspecified application

Species

Unspecified reactive species

Tae-Hee Lee,Colina X Qiao,Vladislav Kuzin,Yuepeng Shi,Marina Farkas,Zhiyan Zhao,Vijayalalitha Ramanarayanan,Tongyu Wu,Tianyi Guan,Xianzhen Zhou,David Corujo,Marcus Buschbeck,Laura Baranello,Philipp Oberdoerffer

Computational and structural biotechnology journal 27:3045-3065 PubMed40687994

2025

Inhibiting MARCH5/Mfn2 signaling as an alternative strategy to protect cardiomyocytes from hypoxia-induced mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Faten Habrat Zoabi,Mulate Zerihun,Roy Lizarovich,Chiara Dalla Torre,Liron Davis,Offir Ertracht,Michal Barsheshet,Shaul Atar,Deborah E Shalev,Marta De Zotti,Hanoch Senderowitz,Nir Qvit

Nucleic acids research 53: PubMed40671527

2025

Sequence, structure, and affinity of miR-34a binding sites determine repression efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Sweetapple,David M Kosek,Elnaz Banijamali,Walter Becker,Juliane Müller,Christina Karadiakos,Lorenzo Baronti,Ileana Guzzetti,Dimitri Schritt,Alan Chen,Emma R Andersson,Katja Petzold

Nucleic acids research 53: PubMed40650974

2025

Regulation of a phage defence island by RptR, a novel repressor that controls restriction-modification systems in diverse bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

YuGeng Zhang,Marion Schuller,Ivan Ahel,Tim R Blower,Rachel M Exley,Christoph M Tang

JCI insight 10: PubMed40627460

2025

Cullin-3 regulates the renal baroreceptor machinery that controls renin gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Golosova,Gaurav Kumar,Ko-Ting Lu,Patricia C Muskus Veitia,Ana Hantke Guixa,Kelsey K Wackman,Eva M Fekete,Daniel T Brozoski,Justin L Grobe,Maria Luisa S Sequeira-Lopez,R Ariel Gomez,Pablo Nakagawa,Curt D Sigmund

Communications chemistry 8:197 PubMed40615626

2025

Chemoproteomics identifies protein ligands for monoacylglycerol lipids.

Applications

Unspecified application

Species

Unspecified reactive species

Karthik Shanbhag,Amol B Mhetre,Ojal Saharan,Archit Devarajan,Anisha Rai,M S Madhusudhan,Harinath Chakrapani,Siddhesh S Kamat

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Anti-6X His tag® antibody [HIS.H8]

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

Related conjugates and formulations (1)

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Carrier free

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Publications (485)

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