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AB300740

Anti-7-Methylguanosine antibody [EPR20846-92]

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal 7-Methylguanosine antibody. Suitable for Dot, FRET and reacts with Modified Nucleic Acid samples. Cited in 5 publications.
5 Images
Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : HeLa total RNA Lane 2 : HeLa mRNA Exposure time : 137s /17s

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : HeLa total RNA Lane 2 : HeLa mRNA Exposure time : 137s /17s

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure times : Lane 1 : 6 seconds; Lanes 2-8 : 180 seconds

FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
  • FRET

Supplier Data

FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab300740 at a concentration range of 0.001-4.000 ug/ml.

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure time : lane 1, 6 sec.; lanes 2-8, 180 sec.

  • Carrier free

    Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20846-92

Isotype

IgG

Carrier free

No

Applications

FRET, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB208196

Anti-1-methyladenosine (m1A) antibody [EPR-19836-208]

RabMAb|Recombinant

Immunoprecipitation - Anti-1-methyladenosine (m1A) antibody [EPR-19836-208] (AB208196)

  • 0
  • 0
Primary Antibodies

AB214727

Anti-5-methylcytosine (5-mC) antibody [RM231]

Recombinant

Immunocytochemistry - Anti-5-methylcytosine (5-mC) antibody [RM231] (AB214727)

  • 0
  • 0
Primary Antibodies

AB208577

Anti-N6-methyladenosine (m6A) antibody [17-3-4-1]

Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (AB208577)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FRET" : {"fullname" : "FRET", "shortname":"FRET"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/500", "Dot-species-notes": "<p></p>", "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "", "FRET-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 7-Methylguanosine also known as 7-methylguanosine cap or 7-methylguanosine triphosphate (7-methylguanosine GTP) plays an important role in RNA processing. It is a modified nucleotide with a mass of approximately 362.3 Da. This cap structure is found at the 5' end of eukaryotic messenger RNA (mRNA). The 7-methylguanosine cap is expressed in the nucleus where it is added co-transcriptionally to nascent mRNA transcripts. This modification enhances mRNA stability and regulates its export to the cytoplasm.
Biological function summary

This cap modification promotes ribosomal binding facilitating efficient translation initiation. The 7-methylguanosine cap is part of the cap-binding complex which includes other proteins like eIF4E. This complex protects RNA from degradation and aids in splicing 5' end processing and polyadenylation. The methylation of the guanine base forms a structure that recruits proteins essential for mRNA maturation and function. Without this modification mRNA would display reduced translational efficiency and be more susceptible to exonucleolytic decay.

Pathways

7-methylguanosine participates in the RNA processing and translation initiation pathways. It interacts with the eukaryotic translation initiation factor 4E (eIF4E) a critical player in the regulation of mRNA cap-dependent translation. The binding of eIF4E to the 7-methylguanosine cap is essential for assembly of the eIF4F complex. This complex drives the mRNA to the ribosome facilitating protein synthesis. Additionally this cap structure influences mRNA export and poly(A) tail formation connecting to the broader mRNA metabolic pathway and impacting gene expression regulation.

Defects in the 7-methylguanosine cap can lead to improper mRNA processing and contribute to diseases like cancer and some congenital disorders. Dysregulation of cap-binding proteins such as eIF4E links to oncogenesis as these proteins can become overexpressed in cancers driving abnormal cell proliferation. Additionally cancer therapies often target these pathways to inhibit the translation of oncogenic mRNAs. Furthermore mutations or malfunctions in methylation enzymes can result in congenital developmental disorders as critical proteins necessary for normal growth and cellular function become deficient due to impaired mRNA translation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 7-Methylguanosine
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 80:100658 PubMed40245789

2025

Kynurenine promotes the immune escape of colorectal cancer cells via NAT10-mediated acC acetylation of PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Zaibiao Wang,Manman Yin,Ruhang Zhou,Ming Li,Jie Peng,Zhengguang Wang

Cell death discovery 11:27 PubMed39870616

2025

METTL1 coordinates cutaneous squamous cell carcinoma progression via the m7G modification of the ATF4 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Xinru Zhang,Tong Chen,Fanrong Zhang,Huanhuan Shi,Xiang Li,Zhijuan Wang,Dong Wang,Chao Hou

Nature communications 15:10224 PubMed39587108

2024

Promotion of TLR7-MyD88-dependent inflammation and autoimmunity in mice through stem-loop changes in Lnc-Atg16l1.

Applications

RIP

Species

Mouse

Zongheng Yang,Shuchen Ji,Lun Liu,Shuo Liu,Bingjing Wang,Yuanwu Ma,Xuetao Cao

Cancer medicine 13:e7420 PubMed38967523

2024

METTL1/FOXM1 promotes lung adenocarcinoma progression and gefitinib resistance by inhibiting PTPN13 expression.

Applications

Dot

Species

Human

Wei Peng,Jia Fu,Lijun Zhou,Huaxin Duan

Heliyon 10:e25599 PubMed38370261

2024

RNA-related DNA damage and repair: The role of N7-methylguanosine in the cell nucleus exposed to UV light.

Applications

Dot, Dot

Species

Human, Mouse

Lenka Stixová,Vlastimil Tichý,Eva Bártová
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com