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AB300741

Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free)

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Rabbit Recombinant Monoclonal 7-Methylguanosine antibody. Carrier free. Suitable for Dot, FRET and reacts with Modified Nucleic Acid samples.

View Alternative Names

m7G

5 Images
Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : HeLa total RNA Lane 2 : HeLa mRNA Exposure time : 137s /17s

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : HeLa total RNA Lane 2 : HeLa mRNA Exposure time : 137s /17s

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure times : Lane 1 : 6 seconds; Lanes 2-8 : 180 seconds

FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
  • FRET

Supplier Data

FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab300740 at a concentration range of 0.001-4.000 ug/ml.

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
  • Dot

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure time : lane 1, 6 sec.; lanes 2-8, 180 sec.

  • Unconjugated

    Anti-7-Methylguanosine antibody [EPR20846-92]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20846-92

Isotype

IgG

Carrier free

Yes

Applications

Dot, FRET

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FRET" : {"fullname" : "FRET", "shortname":"FRET"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "", "FRET-species-notes": "<p></p>" } } }
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Product details

ab300741 is the carrier-free version of ab300740.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 7-Methylguanosine also known as 7-methylguanosine cap or 7-methylguanosine triphosphate (7-methylguanosine GTP) plays an important role in RNA processing. It is a modified nucleotide with a mass of approximately 362.3 Da. This cap structure is found at the 5' end of eukaryotic messenger RNA (mRNA). The 7-methylguanosine cap is expressed in the nucleus where it is added co-transcriptionally to nascent mRNA transcripts. This modification enhances mRNA stability and regulates its export to the cytoplasm.
Biological function summary

This cap modification promotes ribosomal binding facilitating efficient translation initiation. The 7-methylguanosine cap is part of the cap-binding complex which includes other proteins like eIF4E. This complex protects RNA from degradation and aids in splicing 5' end processing and polyadenylation. The methylation of the guanine base forms a structure that recruits proteins essential for mRNA maturation and function. Without this modification mRNA would display reduced translational efficiency and be more susceptible to exonucleolytic decay.

Pathways

7-methylguanosine participates in the RNA processing and translation initiation pathways. It interacts with the eukaryotic translation initiation factor 4E (eIF4E) a critical player in the regulation of mRNA cap-dependent translation. The binding of eIF4E to the 7-methylguanosine cap is essential for assembly of the eIF4F complex. This complex drives the mRNA to the ribosome facilitating protein synthesis. Additionally this cap structure influences mRNA export and poly(A) tail formation connecting to the broader mRNA metabolic pathway and impacting gene expression regulation.

Defects in the 7-methylguanosine cap can lead to improper mRNA processing and contribute to diseases like cancer and some congenital disorders. Dysregulation of cap-binding proteins such as eIF4E links to oncogenesis as these proteins can become overexpressed in cancers driving abnormal cell proliferation. Additionally cancer therapies often target these pathways to inhibit the translation of oncogenic mRNAs. Furthermore mutations or malfunctions in methylation enzymes can result in congenital developmental disorders as critical proteins necessary for normal growth and cellular function become deficient due to impaired mRNA translation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 7-Methylguanosine
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com