AB211498

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal 8-methyladenosine (m8A) antibody. Suitable for IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 2 publications.

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ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (AB211498)

ELISA

Supplier Data

ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (AB211498)

Biotinylated m8A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.

Modified oligonucleotide (5 μM) 5' Biotin-mN.mN.mN.mN.mN.[m8A]*.mN.mN.mN.mN.mN 3'

Unmodified oligonucleotide (5 μM) 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)

m - 2'O methyl protection

* - phosphorothioate protection

ELISA was performed on 1.0 μg/ml of antigen using ab211498 at a concentration range of 0.005-4.000 μg/ml followed by Goat Anti-Rabbit IgG (H+L) alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (AB211498)

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab211498 (0.2 μg) was coated into Dynabeads^® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m8A]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (ab211498)

false

Carrier free

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR-20836-125-1

Isotype

IgG

Carrier free

Applications

ELISA, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Has been developed to discriminate between the modified base 8-methyladenosine (m8A) and the unmodified counterpart Adenosine (A).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 0.2 μg.</p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-4 µg/mL", "ELISA-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

8-Methyladenosine (m8A) represents a modified nucleoside with a methyl group added to the adenosine molecule. It is known also as N-methyladenosine. The mass of m8A depends on its position within a nucleic acid sequence; generally the addition of a methyl group alters its properties without significantly affecting its overall mass. This modification typically occurs within rRNA tRNA and mRNA across many species including humans. The expression of m8A is predominantly associated with cellular RNA but detailed characterization of its distribution within particular tissues or cell types remains an active research area.

Biological function summary

8-methyladenosine modifies RNA and affects various cellular processes. Its presence can influence RNA stability translation efficiency and splicing patterns. Although not part of any known stable complex it cooperates with proteins involved in RNA metabolism such as methyltransferases which mediate its methylation. The dynamic modification of m8A implies a regulatory role in RNA-related processes impacting the cell's ability to respond to different physiological demands.

Pathways

M8A modification integrates into cellular regulatory systems including the RNA processing and decay pathways. It connects with proteins such as methyltransferases which are responsible for adding methyl groups to nucleosides. In RNA processing pathways m8A potentially affects the interactions between RNA and RNA-binding proteins influencing RNA localization and degradation. Its role interplays with the m6A methylation pathway sharing some common methyltransferases and demethylases that adjust methylation marks on RNA.

M8A alteration associates with cancer and neurodegenerative diseases. Abnormal m8A levels may disrupt normal RNA metabolism and contribute to tumorigenesis or cancer progression. Within neurodegenerative diseases m8A modifications in RNA could affect neuronal function and survival linking to proteins involved in RNA transport and degradation. Research efforts continue to uncover how this RNA modification might serve as a biomarker or therapeutic target in various pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 8-methyladenosine (m8A)

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:103 PubMed41035073

2025

Molecular hallmarks of excitatory and inhibitory neuronal resilience to Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Castanho,Pourya Naderi Yeganeh,Carles A Boix,Sarah L Morgan,Hansruedi Mathys,Dmitry Prokopenko,Bartholomew White,Larisa M Soto,Giulia Pegoraro,Saloni Shah,Athanasios Ploumakis,Nikolas Kalavros,David A Bennett,Christoph Lange,Doo Yeon Kim,Lars Bertram,Li-Huei Tsai,Manolis Kellis,Rudolph E Tanzi,Winston Hide

RNA biology 19:1153-1171 PubMed36382943

2022

Early recruitment of PARP-dependent mA RNA methylation at DNA lesions is subsequently accompanied by active DNA demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Soňa Legartová,Alena Svobodová Kovaříková,Jana Běhalová Suchánková,Hana Polášek-Sedláčková,Eva Bártová

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1]

ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (AB211498)

Immunoprecipitation - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (AB211498)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise