AB251531

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 8-methyladenosine (m8A) antibody. Carrier free. Suitable for ELISA, IP and reacts with Modified Nucleic Acid samples.

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ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free (AB251531)

ELISA

Supplier Data

ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free (AB251531)

This data was developed using ab211498, the same antibody clone in a different buffer formulation.

Biotinylated m8A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.

Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m8A]*.mN.mN.mN.mN.mN 3'

Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)

m - 2'O methyl protection

* - phosphorothioate protection

ELISA was performed on 1.0 μg/ml of antigen using ab211498 at a concentration range of 0.005-4.000 μg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free (AB251531)

This data was developed using ab211498, the same antibody clone in a different buffer formulation.

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab211498 (0.2 μg) was coated into Dynabeads^® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.
Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m8A]*.mN.mN.mN.mN.mN 3'
Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (<a href='/en-us/products/primary-antibodies/8-methyladenosine-m8a-antibody-epr-20836-125-1-ab211498'>ab211498</a>)

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Unconjugated

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR-20836-125-1

Isotype

IgG

Carrier free

Yes

Applications

ELISA, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Has been developed to discriminate between the modified base 8-methyladenosine (m8A) and the unmodified counterpart Adenosine (A).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }

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Product details

ab251531 is the carrier-free version of ab211498.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

8-Methyladenosine (m8A) represents a modified nucleoside with a methyl group added to the adenosine molecule. It is known also as N-methyladenosine. The mass of m8A depends on its position within a nucleic acid sequence; generally the addition of a methyl group alters its properties without significantly affecting its overall mass. This modification typically occurs within rRNA tRNA and mRNA across many species including humans. The expression of m8A is predominantly associated with cellular RNA but detailed characterization of its distribution within particular tissues or cell types remains an active research area.

Biological function summary

8-methyladenosine modifies RNA and affects various cellular processes. Its presence can influence RNA stability translation efficiency and splicing patterns. Although not part of any known stable complex it cooperates with proteins involved in RNA metabolism such as methyltransferases which mediate its methylation. The dynamic modification of m8A implies a regulatory role in RNA-related processes impacting the cell's ability to respond to different physiological demands.

Pathways

M8A modification integrates into cellular regulatory systems including the RNA processing and decay pathways. It connects with proteins such as methyltransferases which are responsible for adding methyl groups to nucleosides. In RNA processing pathways m8A potentially affects the interactions between RNA and RNA-binding proteins influencing RNA localization and degradation. Its role interplays with the m6A methylation pathway sharing some common methyltransferases and demethylases that adjust methylation marks on RNA.

M8A alteration associates with cancer and neurodegenerative diseases. Abnormal m8A levels may disrupt normal RNA metabolism and contribute to tumorigenesis or cancer progression. Within neurodegenerative diseases m8A modifications in RNA could affect neuronal function and survival linking to proteins involved in RNA transport and degradation. Research efforts continue to uncover how this RNA modification might serve as a biomarker or therapeutic target in various pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 8-methyladenosine (m8A)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free

ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free (AB251531)

Immunoprecipitation - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] - BSA and Azide free (AB251531)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise